Fly techniques
Preprints describing or showcasing new techniques for Drosophila genetics, molecular biology etc...
List by Gabriel Aughey
Preprints:
Gene knock-ins in Drosophila using homology-independent insertion of universal donor plasmids
https://www.biorxiv.org/content/10.1101/639484v1
Describes tools for creating knock-ins without the requirement for cloning homolgy arms.
A large-scale resource for tissue-specific CRISPR mutagenesis in Drosophila
https://www.biorxiv.org/content/10.1101/636076v1
The authors describe large scale libraries for in vivo CRISPR mutagenesis, as well as a selection of uORF cas9 lines for tuning Cas9 expression, thus mitigating toxicity.
Proximity labeling reveals novel interactomes in live Drosophila tissue
https://www.biorxiv.org/content/10.1101/542795v1
Demonstration of proximity labelling approach for identifying protein-protein interactions in vivo.
Enhanced Golic+: Gene targeting with 100% recovery in Drosophila male germ cells
https://www.biorxiv.org/content/10.1101/681874v1.full
Improved methods for HDR in drosophila with impressive 100% recovery rate of desired insertion from male germ cells.
Posted on: 21 May 2019 , updated on: 1 July 2019
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