Preprints describing or showcasing new techniques for Drosophila genetics, molecular biology etc...List by Gabriel Aughey
Gene knock-ins in Drosophila using homology-independent insertion of universal donor plasmids
Describes tools for creating knock-ins without the requirement for cloning homolgy arms.
A large-scale resource for tissue-specific CRISPR mutagenesis in Drosophila
The authors describe large scale libraries for in vivo CRISPR mutagenesis, as well as a selection of uORF cas9 lines for tuning Cas9 expression, thus mitigating toxicity.
Proximity labeling reveals novel interactomes in live Drosophila tissue
Demonstration of proximity labelling approach for identifying protein-protein interactions in vivo.
Enhanced Golic+: Gene targeting with 100% recovery in Drosophila male germ cells
Improved methods for HDR in drosophila with impressive 100% recovery rate of desired insertion from male germ cells.
Posted on: 21 May 2019 , updated on: 1 July 2019