Structure of an infectious mammalian prion
Preprint posted on February 15, 2021 https://www.biorxiv.org/content/10.1101/2021.02.14.431014v1
Neurodegenerative diseases are highly debilitating conditions. Collectively, they cause a gradual degeneration of the central or peripheral nervous system, with symptoms ranging from memory loss, mood swings and behavioural changes to a loss of movement (depending upon the locations of the affected nerves; 1). What triggers neurodegeneration is still widely investigated however a group of such disorders (for example Creutzfeldt-Jakob Disease; CJD) have been linked to lethal prion pathogens. Prions are very unusual pathogens as, unlike viruses, bacteria and parasites, they do not contain DNA or RNA. Instead, prions are infectious, misfolded assemblies of a normal cellular prion protein (PrPC), with the resulting protein known as PrPSC. Prions can self-propagate, meaning PrP monomers assemble as fibrils, which can fragment then initiate the formation of other fibrils (2). They transmit in various ways, for example via blood transfusion and infected tissue/other fluids, consumption of infected tissues or from the environment from contaminated soil (3). The pathology of the infection arises when the prion form of the protein forms aggregated (often in fibrillar forms called amyloids) which accumulate and fatally damage tissue; a similar pathology can be observed in diseases such as Parkinson’s and Alzheimer’s disease (4). As development of a prion disease is centred on the way in which the PrPC folds in the host organism, understanding how prions fold and multiply is key to understanding disease formation, transmission and manifestation. Here, the authors perform Cryo-EM to produce the first high-resolution structure of an infectious mammalian prion. This prion strain (named 263K) was purified from the brains of terminally ill hamsters.
PrPC is a cell surface protein attached to the surface via a glycosylphosphatidylinositol (GPI) anchor. The N-terminal section is disordered and flexible whereas the C-terminal is made up of 3 alpha-helical domains, and a small beta-sheet. PrPC is both protease sensitive (i.e can be digested) and detergent soluble which is entirely contrary to the prion form (PrPSC) of the protein which is highly resistant to both. PrPSC assembles as beta-sheet rich fibres, that can appear like twisted ribbons or intertwined filaments. This arrangement is starkly different to the arrangement of the original non-prion form of the protein which is largely alpha helical with a very low beta-sheet content (5). In this study, the authors use cryogenic electron microscopy (Cryo-EM) to produce a structure of the prion form of the protein. Cryo-EM is a sophisticated, high resolution microscopy technique in which samples can be imaged in their native hydrated state. Samples are ‘flash frozen’ in a cryogenic (extremely low temperature) solution, for example, liquid ethane. This super rapid freezing means water molecules within the sample will be vitrified rather than crystallized, instead leaving a solid sample in its native hydrated state which can be imaged at high resolution on a transmission electron microscope (which uses a high-energy particle beam to capture the pattern of electron scatter generated when the beam passes through the sample; 6).
A selection of figures from Kraus et al. Fig. 1A. Negative stained TEM images of 263K prion fibrils (scale bar = 50 nm). Fig 1B. 2D cryo-EM images of 263K prion fibrils. Orange arrows = regular 4.9 Å spacing, white arrows = globules. Fig. 1E. A project of the fibril density map. Fig. 2A. An extended model of the fibril shown as a ribbon diagram. Fig. 2D. The stacking of beta-sheets within a region of the fibril. Different motifs are labelled and depicted by dashed lines. Yellow spheres = disulfide bond. Purple stars = position of the enlarged region on the fibril. Figures reproduced under a CC0 license.
- Using Cryo-EM, the authors produce a high-resolution structure (3.1 Å) of a mammalian infective prion. They solve the structure of an infectious prion isolated from hamster brains infected with the prion strain 263K, which was derived initially from sheep with the lethal neurodegenerative condition called Scrapie.
- The structure of 263K prion fibrils reveals their arrangement as left-hand twisted (>99%) fibrils between 13-20 nm in width at the widest point of the fibre cross-section and ~ 3.5 nm at the narrowest, in keeping with various prior studies on prion structure. From the start of one twist to another was ~ 100 nm. In ~ 45% of fibrils, globules were identified on one side of the fibril, sometimes spanning the entire length. The authors are unsure as to whether these structures are artifices occurring during sample preparation.
- The structure of the infectious prion 263K appears to be very different to that of synthetic recombinant human PrP amyloid fibrils, which have much smaller ordered cores and are therefore likely to be non-infectious.
- The authors resolved different motifs within the originally disordered N-terminal region, which were unable to be resolved in non-infectious PrPC fibrils from previous studies. These motifs included a beta-arch, and a Greek key motif (which is a serpentine fold containing beta sheets and loops). The C-terminal region had another beta-arch which, in this case, was disulfide-linked and heavily glycosylated. The authors suggest the combination of these features may contribute to the infectivity of prion 263K as one or more were absent in the non-prion PrP fibrils.
- They show that the location of the GPI anchors (at one side of the fibril) might help them to bind to membranes. This means the fibrils could be wrapped up in host membranes, which might contribute to pathognomonic features of prion pathology. The exposed (non-wrapped up) ends could be the site associated with refolding of the normal protein into the prion isoform.
To date, two major structural models have been proposed for infectious prion organisation, 1) arranged as a 4-rung beta solenoid like structure or 2) a Parallel in register intermolecular beta sheet (PIRIBS) where monomers are aligned and stacked.
- The authors showed that the 263K prion has a PIRIBS-based structure; further studies will be needed to determine if other prion strains have PIRIBS architectures as well, or in some cases, beta solenoids.
What I liked about this preprint:
I have always been fascinated by prion pathologies. It is interesting to think that misfolding of a protein can cause such devastating diseases. Given this, understanding what prions look like once they become pathogenic and how they become pathogenic by altering their shape is very important to understand how they arise and replicate. Cryo-EM is a great technique for this purpose. The author’s data shows they have been able to isolate a prion structure, build a high-resolution model, and reveal some peculiarities about the organisation of their prion contributing to our knowledge on prion structure.
Questions for the authors:
Q1: Prion fibrils can be very hard to isolate due to resistance of these proteins to proteases and detergents. What challenges did you face during the extraction and isolation of the 263K prion?
Q2: PrPSC is a highly glycosylated structure. It has been argued that such glycosylation could only be accommodated with beta-solenoid structures, but having now solved the structure of 263K at high-resolution, you show that 263K has a PIRIBS-based structure. Other mammalian prions, for example from mice, have been predicted (though not directly shown) to have a beta-solenoid-based architecture. Why do you think there is a difference between your prion structure and the beta-solenoid model hypothesised for murine prions? Do you think prion structure varies in this manner due to the host species? Or could it be previous studies have performed cryo-EM on anchorless molecules i.e does having the anchor drastically alter the structural configuration?
Q3: What is the role of the Greek key motif in this prion (is this known?) and how might this play a role in altering the conformation of PrPC to PrPSC?
- Gitler, A.D., Dhillon, P., and Shorter, J. Neurodegenerative disease: models, mechanisms, and a new hope. Dis Model Mech. (2017).
- Collinge, J., and Clarke, A. A general model of prion strains and their pathogenicity. Science. (2007).
- Lodhi, S.K., Mirza, M., and Khawaja, A.M. Prions Disease: A review of novel transmission methods and efforts at discovering preventative and therapeutic modalities. Infectious Dis. In Clin Pract. (2018).
- Kulenkampff, K., Perez, A.M.W., Sormanni, P., Habchi, J., and Vendruscolo, M. Quantifying misfolded protein oligomers as drug targets and biomarkers in Alzheimer and Parkinson diseases. Rev. Chem. (2021).
- Mastriani, J.A. Chapter 21- Prion Diseases. Rosenberg’s Molecular and Genetic Basis of Neurological and Psychiatric Disease (Firth Edition). (2015).
- Murata, K., and Wolf, M. Cryo-electron microscopy for structural analysis of dynamic biological macromolecules. (2018).
Posted on: 4th March 2021Read preprint
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