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EMBL Seeing is Believing – Imaging the Molecular Processes of Life

Preprints discussed at the 2019 edition of Seeing is Believing, at EMBL Heidelberg from the 9th-12th October 2019

List by Dey Lab

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Differential spatiotemporal targeting of Toxoplasma and Salmonella by GBP1 assembles caspase signalling platforms

Daniel Fisch, Barbara Clough, Marie-Charlotte Domart, Hironori Bando, Tereza Masonou, Lucy M Collinson, Masahiro Yamamoto, Avinash R Shenoy, Eva-Maria Frickel

https://www.biorxiv.org/content/10.1101/792804v1

Direct Arp2/3-vinculin binding is essential for cell spreading, but only on compliant substrates and in 3D

Tadamoto Isogai, Kevin M. Dean, Philippe Roudot, Qiuyan Shao, Justin D. Cillay, Erik. S. Welf, Meghan K. Driscoll, Shaina P. Royer, Nikhil Mittal, Bo-Jui Chang, Sangyoon J. Han, Reto Fiolka, Gaudenz Danuser

https://www.biorxiv.org/content/10.1101/756718v1

Formation of talin-vinculin pre-complexes dictates maturation of nascent adhesions by accelerated force transmission and vinculin recruitment

Sangyoon J. Han, Kevin M. Dean, Austin J. Whitewood, Alexia Bachir, Edgar Guttierrez, Alex Groisman, Alan R. Horwitz, Benjamin T. Goult, Gaudenz Danuser

https://www.biorxiv.org/content/10.1101/735183v1

Dense neuronal reconstruction through X-ray holographic nano-tomography

Alexandra Pacureanu, Jasper Maniates-Selvin, Aaron T. Kuan, Logan A. Thomas, Chiao-Lin Chen, Peter Cloetens, Wei-Chung Allen Lee

https://www.biorxiv.org/content/10.1101/653188v1

Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies

Mauricio Toro-Nahuelpan, Ievgeniia Zagoriy, Fabrice Senger, Laurent Blanchoin, Manuel Théry, Julia Mahamid

https://www.biorxiv.org/content/10.1101/676189v1

Correlative three-dimensional super-resolution and block face electron microscopy of whole vitreously frozen cells

David P. Hoffman, Gleb Shtengel, C. Shan Xu, Kirby R. Campbell, Melanie Freeman, Lei Wang, Daniel E. Milkie, H. Amalia Pasolli, Nirmala Iyer, John A. Bogovic, Daniel R. Stabley, Abbas Shirinifard, Song Pang, David Peale, Kathy Schaefer, Wim Pomp, Chi-Lun Chang, Jennifer Lippincott-Schwartz, Tom Kirchhausen, David J. Solecki, Eric Betzig, Harald Hess

https://www.biorxiv.org/content/10.1101/773986v1

Instantaneous isotropic volumetric imaging of fast biological processes

Nils Wagner, Nils Norlin, Jakob Gierten, Gustavo de Medeiros, Bálint Balázs, Joachim Wittbrodt, Lars Hufnagel, Robert Prevedel

https://www.biorxiv.org/content/10.1101/459370v1

Light-sheet engineering using the Field Synthesis theorem

Bo-Jui Chang, Reto Fiolka

https://www.biorxiv.org/content/10.1101/700633v2

Sub-Nanometer Precision using Bayesian Grouping of Localizations

Mohamadreza Fazel, Michael J. Wester, Bernd Rieger, Ralf Jungmann, Keith A. Lidke

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Computational Correction of Spatially-Variant Optical Aberrations in 3D Single Molecule Localization Microscopy

Ting Yan, Charles J. Richardson, Mingxing Zhang, Andreas Gahlmann

https://www.biorxiv.org/content/10.1101/504712v1

Profiling cellular diversity in sponges informs animal cell type and nervous system evolution

Jacob M. Musser, Klaske J. Schippers, Michael Nickel, Giulia Mizzon, Andrea B. Kohn, Constantin Pape, Jörg U. Hammel, Florian Wolf, Cong Liang, Ana Hernández-Plaza, Kaia Achim, Nicole L. Schieber, Warren R. Francis, Sergio Vargas R., Svenja Kling, Maike Renkert, Roberto Feuda, Imre Gaspar, Pawel Burkhardt, Peer Bork, Martin Beck, Anna Kreshuk, Gert Wörheide, Jaime Huerta-Cepas, Yannick Schwab, Leonid L. Moroz, Detlev Arendt

https://www.biorxiv.org/content/10.1101/758276v1

A unified role for membrane-cortex detachment during cell protrusion initiation

Erik S. Welf, Christopher E. Miles, Jaewon Huh, Meghan K. Driscoll, Tadamoto Isogai, Jungsik Noh, Andrew D. Weems, Joseph Chi, Theresa Pohlkamp, Kevin Dean, Reto Fiolka, Alex Mogilner, Gaudenz Danuser

https://www.biorxiv.org/content/10.1101/696211v2

An ancestral role of pericentrin in centriole formation through SAS-6 recruitment

Daisuke Ito, Sihem Zitouni, Swadhin Chandra Jana, Paulo Duarte, Jaroslaw Surkont, Zita Carvalho-Santos, José B. Pereira-Leal, Miguel Godinho Ferreira, Mónica Bettencourt-Dias

https://www.biorxiv.org/content/10.1101/313494v1

A role for liquid-liquid phase separation in ESCRT-mediated nuclear envelope reformation

Alexander von Appen, Dollie LaJoie, Isabel E. Johnson, Mike Trnka, Sarah M. Pick, Alma L. Burlingame, Katharine S. Ullman, Adam Frost

https://www.biorxiv.org/content/10.1101/577460v1

Modular actin nano-architecture enables podosome protrusion and mechanosensing

Koen van den Dries, Leila Nahidiazar, Johan A. Slotman, Marjolein B.M. Meddens, Elvis Pandzic, Ben Joosten, Marleen Ansems, Joost Schouwstra, Anke Meijer, Raymond Steen, Mietske Wijers, Jack Fransen, Adriaan B. Houtsmuller, Paul W. Wiseman, Kees Jalink, Alessandra cambi

https://www.biorxiv.org/content/10.1101/583492v1

ER membranes exhibit phase behavior at sites of organelle contact

Christopher King, Prabuddha Sengupta, Arnold Seo, Jennifer Lippincott-Schwartz

https://www.biorxiv.org/content/10.1101/707505v1

3D super-resolution deep-tissue imaging in living mice

Mary Grace M. Velasco, Mengyang Zhang, Jacopo Antonello, Peng Yuan, Edward S. Allgeyer, Dennis May, Ons M’Saad, Phylicia Kidd, Andrew E. S. Barentine, Valentina Greco, Jaime Grutzendler, Martin J. Booth, Joerg Bewersdorf

https://www.biorxiv.org/content/10.1101/790212v1

Nanoscale subcellular architecture revealed by multicolor 3D salvaged fluorescence imaging

Yongdeng Zhang, Lena K. Schroeder, Mark D. Lessard, Phylicia Kidd, Jeeyun Chung, Yuanbin Song, Lorena Benedetti, Yiming Li, Jonas Ries, Pietro De Camilli, James E. Rothman, David Baddeley, Joerg Bewersdorf

https://www.biorxiv.org/content/10.1101/613174v1

A general strategy to develop cell permeable and fluorogenic probes for multi-colour nanoscopy

Lu Wang, Mai Tran, Elisa D’Este, Julia Roberti, Birgit Koch, Lin Xue, Kai Johnsson

https://www.biorxiv.org/content/10.1101/690867v1

Chemogenetic Control of Nanobodies

Helen Farrants, Miroslaw Tarnawski, Thorsten G. Müller, Shotaro Otsuka, Julien Hiblot, Birgit Koch, Moritz Kueblbeck, Hans-Georg Kräusslich, Jan Ellenberg, Kai Johnsson

https://www.biorxiv.org/content/10.1101/683557v1

GenEPi: Piezo1-based fluorescent reporter for visualizing mechanical stimuli with high spatiotemporal resolution

Sine Yaganoglu, Nordine Helassa, Benjamin M. Gaub, Maaike Welling, Jian Shi, Daniel J. Müller, Katalin Török, Periklis Pantazis

https://www.biorxiv.org/content/10.1101/702423v1

Biodegradable harmonophores for targeted high-resolution in vivo tumor imaging

Ali Yasin Sonay, Sine Yaganoglu, Martina Konantz, Claire Teulon, Sandro Sieber, Shuai Jiang, Shahed Behzadi, Daniel Crespy, Katharina Landfester, Sylvie Roke, Claudia Langerke, Periklis Pantazis

https://www.biorxiv.org/content/10.1101/694760v1

Direct comparison of clathrin-mediated endocytosis in budding and fission yeast reveals conserved and evolvable features

Yidi Sun, Johnannes Schoeneberg, Shirley Chen, Tommy Jiang, Charlotte Kaplan, Ke Xu, Thomas D. Pollard, David G. Drubin

https://www.biorxiv.org/content/10.1101/733543v1

Cristae undergo continuous cycles of fusion and fission in a MICOS-dependent manner

Arun Kumar Kondadi, Ruchika Anand, Sebastian Hänsch, Jennifer Urbach, Thomas Zobel, Dane M. Wolf, Mayuko Segawa, Marc Liesa, Orian S. Shirihai, Stefanie Weidtkamp-Peters, Andreas S. Reichert

https://www.biorxiv.org/content/10.1101/654541v1

Multi-color single molecule imaging uncovers extensive heterogeneity in mRNA decoding

Sanne Boersma, Deepak Khuperkar, Bram M.P. Verhagen, Stijn Sonneveld, Jonathan B. Grimm, Luke D. Lavis, Marvin E. Tanenbaum

https://www.biorxiv.org/content/10.1101/477661v1

Categories: biochemistry , bioengineering , biophysics , cell biology , developmental biology , microbiology , systems biology

 

Posted on: 11th October 2019 , updated on: 8th November 2019

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