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3D Gastruloids

A curated list of preprints related to Gastruloids (in vitro models of early development obtained by 3D aggregation of embryonic cells). Preprint missing? Don't hesitate to let us know.

List by Paul Gerald L. Sanchez and Stefano Vianello

Preprints:

In vitro teratogenicity testing using a 3D, embryo-like gastruloid system

Veronika Mantziou, Peter Baillie-Benson, Manuela Jaklin, Stefan Kustermann, Alfonso Martinez Arias, Naomi Moris

https://www.biorxiv.org/content/10.1101/2021.03.30.437698v1

Gastruloids as in vitro models of embryonic blood development with spatial and temporal resolution

Giuliana Rossi, Sonja Giger, Tania Hübscher, Matthias P. Lutolf

https://doi.org/10.1101/2021.03.21.436320

Dynamics of anteroposterior axis establishment in a mammalian embryo-like system

Kerim Anlaş, Nicola Gritti, David Oriola, Krisztina Arató, Fumio Nakaki, Jia Le Lim, James Sharpe, Vikas Trivedi

https://www.biorxiv.org/content/10.1101/2021.02.24.432766v1

The authors detail the initial symmetry breaking of Gastruloids, and explore systematically the cellular and biochemical parameters influencing this event. Furthermore, they profile early Gastruloids at single cell resolution and – by comparison with embryonic datasets – highlight important differences between modes of development executed by cells in Gastruloids compared to the embryo.

Bioengineered embryoids mimic post-implantation development in vitro

Mehmet Girgin, Nicolas Broguiere, Sylke Hoehnel, Nathalie Brandenberg, Bastien Mercier, Alfonso Martinez-Arias, Matthias P Lutolf

https://www.biorxiv.org/content/10.1101/2021.01.10.426096v1

Integrating central and peripheral neurons in elongating multi-lineage-organized gastruloids

Zachary T Olmsted, Janet L Paluh

https://www.biorxiv.org/content/10.1101/2020.12.29.424774v1

Human Gastruloids develop  central- and peripheral nervous system cell types, and are used as an in vitro platform to test neuromodulators

A FGF2-mediated incoherent feedforward loop induces Erk inhibition and promotes naïve pluripotency

Borzo Gharibi, Emanuel Gonçalves, Buhe Nashun, Alex Montoya, Katherine Mankalow, Stephanie Strohbuecker, Rahuman S M Sheriff, Alessandro Cicarrelli, Joana Carvalho, Emma Nye, Holger Kramer, Ian Rosewell, Petra Hajkova, Pedro Beltrao, Silvia D. M. Santos

https://www.biorxiv.org/content/10.1101/2020.11.11.378869v1

The study briefly employs mouse Gastruloids as an experimental platform to test the lineage potential of different stem cell populations.

Gastruloids generated without exogenous Wnt activation develop anterior neural tissues

Mehmet U. Girgin, Matthias P. Lutolf

https://www.biorxiv.org/content/10.1101/2020.10.10.334326v1

Description of the development of anterior neural identities in mouse Gastruloids by withdrawing Wnt stimulation

Embryological manipulation to probe early evo-devo in the fish Astyanax mexicanus

Jorge Torres-Paz, Sylvie Rétaux

https://www.biorxiv.org/content/10.1101/2020.10.06.328500v1

Unique use of fish Gastruloids as a tool to probe eco-evo-devo questions in Mexican cavefish development.

An highlight of this preprint, with author comments, is available here

In vitro endoderm emergence and self-organisation in the absence of extraembryonic tissues and embryonic architecture

Stefano Vianello, Matthias P. Lutolf

https://doi.org/10.1101/2020.06.07.138883

Description of the early emergence of endoderm progenitor markers within mouse Gastruloids,  their later self-organisation and morphogenesis as epithelial tubes, and the Anterior-Posterior patterning of such endodermal domain. 

Cell state transitions and collective cell movement generate an endoderm-like region in gastruloids

Ali Hashmi, Sham Tlili, Pierre Perrin, Alfonso Martinez Arias, Pierre-Francois Lenne

https://www.biorxiv.org/content/10.1101/2020.05.21.105551v1

First detailed characterisation of the cellular movements associated with mouse Gastruloid morphogenesis. Focus is given to endodermal cells, and the epithelial clusters they appear to be associated with.

Axial Elongation of Caudalized Human Pluripotent Stem Cell Organoids Mimics Neural Tube Development

Ashley R G Libby, David A Joy, Nicholas H Elder, Emily A Bulger, Martina Z Krakora, Eliza A Gaylord, Federico Mendoza-Camacho, Todd C McDevitt

https://www.biorxiv.org/content/10.1101/2020.03.05.979732v1

Description of the development of human Gastruloids, and of structures analogous to a neural tube within them.

Mouse embryonic stem cells self-organize into trunk-like structures with neural tube and somites

Jesse V Veenvliet, Adriano Bolondi, Helene Kretzmer, Leah Haut, Manuela Scholze-Wittler, Dennis Schifferl, Frederic Koch, Milena Pustet, Simon Heimann, Rene Buschow, Lars Wittler, Bernd Timmermann, Alexander Meissner, Bernhard G Herrmann

https://www.biorxiv.org/content/10.1101/2020.03.04.974949v1

Somite condensation and other morphological transformations can be induced by exposing mouse Gastruloids to extracellular matrix components. Gastruloids develop into so-called “Trunk-like Structures”

An highlight of this preprint is available here

Early neurulation recapitulated in assemblies of embryonic and extraembryonic cells

Noémie M. L. P. Bérenger-Currias, Maria Mircea, Esmée Adegeest, Patrick R. van den Berg, Marleen Feliksik, Mazène Hochane, Timon Idema, Sander J. Tans, Stefan Semrau

https://www.biorxiv.org/content/10.1101/2020.02.13.947655v1

First use of extraembryonic cells (extraembryonic endoderm) to induce the development of anterior and neural cell types from mouse Gastruloids

An highlight of this preprint, with author comments, is available here

Embryonic organoids recapitulate early heart organogenesis

Giuliana Rossi, Andrea Boni, Romain Guiet, Mehmet Girgin, Robert G. Kelly, Matthias P. Lutolf

https://www.biorxiv.org/content/10.1101/802181v3

Description of the development and organisation of cardiac fates in mouse Gastruloids

Self-organised symmetry breaking in zebrafish reveals feedback from morphogenesis to pattern formation

Vikas Trivedi, Timothy Fulton, Andrea Attardi, Kerim Anlas, Chaitanya Dingare, Alfonso Martinez-Arias, Benjamin Steventon

https://www.biorxiv.org/content/10.1101/769257v2

Generation of zebrafish Gastruloids (“pescoids”), and description of their development in vitro

An highlight of this preprint, with author comments, is available here

Emergence and patterning dynamics of mouse definitive endoderm

Maayan Pour, Abhishek Sampath Kumar, Maria Walther, Lars Wittler, Alexander Meissner, Iftach Nachman

https://www.biorxiv.org/content/10.1101/728642v1

First detailed description of the timing and pattern of emergence of endoderm cells in mouse Gastruloids

A mesoderm-independent role for Nodal signaling in convergence & extension gastrulation movements

Margot L.K. Williams, Lilianna Solnica-Krezel

https://www.biorxiv.org/content/10.1101/671164v2

Zebrafish Gastruloids used as an experimental tool to test the intrinsic and extrinsic requirements for convergent extension movements in the fish embryo

Gastruloid development competence discriminates different states of pluripotency between naïve and primed

Federica Cermola, Cristina D’Aniello, Rosarita Tatè, Dario De Cesare, Alfonso Martinez-Arias, Gabriella Minchiotti, Eduardo Jorge Patriarca

https://www.biorxiv.org/content/10.1101/664920v1

Mouse Gastruloids deployed as an experimental platform to test and discriminate between different stem cell potency states

Prediction and control of symmetry breaking in embryoid bodies by environment and signal integration

Naor Sagy, Shaked Slovin, Maya Allalouf, Maayan Pour, Gaya Savyon, Jonathan Boxman, Iftach Nachman

https://www.biorxiv.org/content/10.1101/506543v1

First evidence that the symmetry-breaking event in mouse Gastruloids may integrate not only biochemical/genetic inputs, but also the local mechanical environment

The chick caudo-lateral epiblast acts as a permissive niche for generating neuromesodermal progenitor behaviours

Peter Baillie-Johnson, Octavian Voiculescu, Penny Hayward, Benjamin Steventon

https://www.biorxiv.org/content/10.1101/243980v1.full

Mouse Gastruloids are used as an experimental source of cells (NMPs and others) to use in transplantation experiments

Gastruloids develop the three body axes in the absence of extraembryonic tissues and spatially localised signalling

D.A. Turner, L. Alonso-Crisostomo, M. Girgin, P. Baillie-Johnson, C. R. Glodowski, P. C. Hayward, J. Collignon, C. Gustavsen, P. Serup, B. Steventon, M. Lutolf, A. Martinez Arias

https://www.biorxiv.org/content/10.1101/104539v1

Evidence for the existence of all three major developmental axes (Anterior-Posterior, Dorso-Ventral, Left-Right) in mouse Gastruloids

Interactions between Nodal and Wnt signalling Drive Robust Symmetry-Breaking and Axial Organisation in Gastruloids (Embryonic Organoids)

D.A. Turner, C.R. Glodowski, L. Alonso-Crisostomo, P. Baillie-Johnson, P.C. Hayward, J. Collignon, C. Gustavsen, P. Serup, C. Schröter, A. Martinez Arias

https://www.biorxiv.org/content/10.1101/051722v2

Description and quantification of the activity of key signalling pathways as mouse Gastruloids develop. First attempts at matching Gastruloid development with mouse embryonic stages

Generation of Aggregates of Mouse ES Cells that Show Symmetry Breaking, Polarisation and Emergent Collective Behaviour in vitro

Peter Baillie-Johnson, Susanne C. van den Brink, Tina Balayo, David A. Turner, Alfonso Martinez Arias

https://www.biorxiv.org/content/10.1101/005215v2

A description of the original protocol for the generation of Gastruloids from mouse embryonic stem cells

Categories: bioengineering , cell biology , developmental biology

Tags: gastruloids

Posted on: 8th March 2020 , updated on: 1st April 2021

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