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February in preprints – the CellBio edition

A group of preLighters, with expertise in different areas of cell biology, have worked together to create this preprint reading lists for researchers with an interest in cell biology. This month, categories include: 1) biochemistry and cell metabolism 2) cell organelles and organisation 3) cell signalling, migration and mechanosensing

List by Barbora Knotkova, Matthew Davies, Sristilekha Nath, Girish Kale, Prasanna Padmanaban, Vibha SINGH, Felipe Del Valle Batalla

Preprints:

Biochemistry & Cell Metabolism

Solubilization of Membrane Proteins using designed protein WRAPS

Ljubica Mihaljević, David E. Kim, Helen E. Eisenach, Pooja D. Bandawane, Andrew J. Borst, Alexis Courbet, Everton Bettin, Qiushi Liu, Connor Weidle, Sagardip Majumder, Xinting Li, Mila Lamb, Analisa Nicole Azcárraga Murray, Rashmi Ravichandran, Elizabeth C. Williams, Shuyuan Hu, Lynda Stuart, Linda Grillová, Nicholas R. Thomson, Pengxiang Chang, Melissa J. Caimano, Kelly L. Hawley, Neil P. King, David Baker

https://www.biorxiv.org/content/10.1101/2025.02.04.636539v1

A new method to express and purify recombinant membrane proteins outside of a detergent- or membrane environment by fusing target membrane protein to a custom-designed protein coat that shields the hydrophobic transmembrane region.

Picked by: Barbora Knotkova

From Mihaljević et al. (2025). This image is made available under a CC-BY-NC-ND 4.0 International license.

VDAC isoforms play distinct roles in modulating metabolism and maintaining mitochondrial function

Megha Rajendran, William M. Rosencrans, Wendy Fitzgerald, Diana Huynh, Bethel G. Beyene, Baiyi Quan, Julie Hwang, Nina Bautista, Tsui-Fen Chou, Sergey M. Bezrukov, Tatiana K. Rostovtseva

https://www.biorxiv.org/content/10.1101/2025.02.20.639106v1.full

Individual knock-outs of all three isoforms of the mitochondrial outer membrane channel VDAC in HeLa cells show that each isoform has a different impact on the mitochondrial proteome and on cellular metabolism. Surprisingly, only VDAC3 promotes mitochondrial respiration.

Picked by: Barbora Knotkova

Cell organelles and organisation

Desmoplakin tail domain position in the desmosomal plaque is isoform dependent

Collin M. Ainslie, Krishna Patel, Yen T. B. Tran, Navaneetha Krishnan Bharathan, Volker Spindler, Alexa L. Mattheyses

https://www.biorxiv.org/content/10.1101/2025.02.03.636241v1

This preprint looks at the influence of different desmoplakin isoforms on desmosome organisation. These isoforms have different lengths, due to differences in lengths of their Rod domains. Rod domain length has been suspected to influence protein function, e.g. of the spectraplakin family, and this preprint provides some answers in this regard.

Picked by: Matthew Davies

From Ainslie et al. (2025). This image is made available under a CC-BY 4.0 International license.

On-demand delivery of fibulin-1 enables repeated basement membrane stretching

Adam W.J. Soh, Michael R. Arnwine, Claire A. Gianakas, Zachary D. Clark, Qiuyi Chi, Erin J. Cram, Brenton D. Hoffman, David R. Sherwood

https://www.biorxiv.org/content/10.1101/2025.02.02.636120v1

This preprint emphasizes the crucial role of fibulin-1, a basement membrane (BM) component, which, through its interaction with collagen type IV, supports the spermatheca, a structure that undergoes repeated cycles of BM stretching and expansion during ovulation. The targeted delivery of fibulin-1 specifically during ovulation ensures the integrity and shape of the spermatheca.

Picked by: Sristilekha Nath

Dynamic regulation of Sec24C by phosphorylation and O-GlcNAcylation during cell cycle progression

George R. Georgiou, Tetsuya Hirata, Erik Soderblom, Rose Homoelle, Jakob Maiwald, Michael Boyce

https://www.biorxiv.org/content/10.1101/2025.02.12.638000v1.full

The authors identify changes in post translational modifications of the COPII coat protein Sec24C upon mitotic entry. These modifications relocalise Sec24C away from ER exit sites and therefore likely play a role in halting secretion and aiding equal distribution of the endomembrane system between daughter cells during division.

Picked by: Barbora Knotkova

From Georgiou et al. (2025). This image is made available under a CC-BY-NC-ND 4.0 International license.

Polarity reversal of stable microtubules during neuronal development

Malina K. Iwanski, Albert K. Serweta, H. Noor Verwei, Bronte C. Donders, Lukas C. Kapitein

https://www.biorxiv.org/content/10.1101/2025.02.07.636940v1

The authors of this preprint investigate the establishment of characteristic microtuble polarity of dendrites. They find that early in development, microtubules can exchanged between dendrites and loop back on themselves to correct their orientation.

Picked by: Matthew Davies

Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties

Suyash Naik, Yann-Edwin Keta, Kornelija Pranjic-Ferscha, Édouard Hannezo, Silke Henkes, Carl-Philipp Heisenberg

https://doi.org/10.1101/2025.02.14.638262

The authors identify a keratin-mediated mechanism regulating tissue spreading in zebrafish embryos. During epiboly, actomyosin-driven stress build-up triggers keratin network accumulation, increasing viscosity to maintain tissue integrity and balance forces within the spreading tissue.

Picked by: Sristilekha Nath

From Naik et al. (2025). This image is made available under a CC-BY-ND 4.0 International license.

Pex30-dependent membrane contacts sites maintain ER lipid homeostasis

Joana Veríssimo Ferreira, Yara Ahmed, Tiaan Heunis, Aamna Jain, Errin Johnson, Markus Raeschle, Robert Ernst, Stefano Vanni, Pedro Carvalho

https://www.biorxiv.org/content/10.1101/2025.02.12.637848v1.full

This preprint characterises previously enigmatic domains of the yeast ER-resident protein Pex30 found at ER membrane contact sites with various other organelles. Using a combination of in vivoin vitro and in silico experiments, the authors show that Pex30 is required for phosphatidic acid homeostasis and identify phosphorylation sites that mediate membrane contact site formation based on the metabolic state of the cells.

Picked by: Barbora Knotkova

The neuron-intrinsic membrane skeleton is required for motor neuron integrity throughout lifespan

Carrie Ann Davison, Daniela Garcia, Chengye Feng, Hongyan Hao, Erik M. Jorgensen, Marc Hammarlund

https://www.biorxiv.org/content/10.1101/2025.02.23.639536v1

The authors of this preprint study the requirement of the membrane periodic skeleton in C. elegans motor neurons throughout their life. They find that loss of its integral component, Beta-Spectrin, at all stages of life causes axon breakage and degeneration, revealing a new means to screen for factors required for axon degeneration and maintenance.

Picked by: Matthew Davies

Genetically encoded reporters of actin filament organization in living cells and tissues

Carla Silva Martins, François Iv, Shashi Kumar Suman, Thomas C. Panagiotou, Clara Sidor, María Ruso-López, Camille N. Plancke, Shizue Omi, Rebecca Pagès, Maxime Gomes, Alexander Llewellyn, Sourish Reddy Bandi, Laurie Ramond, Federica Arbizzani, Caio Vaz Rimoli, Frank Schnorrer, François Robin, Andrew Wilde, Loïc LeGoff, Jean-Denis Pedelacq, Antoine Jégou, Stéphanie Cabantous, Sergio A. Rincon, Cristel Chandre, Sophie Brasselet, Manos Mavrakis

https://www.biorxiv.org/content/10.1101/2024.04.26.591305v2

Existing actin probes only poorly represent the organisation of actin filaments. In this preprint, the authors generate new probes that allow distinction of actin filaments via fluorescence polarisation microscopy. This marks a significant advance in actin visualisation.

Picked by: Matthew Davies

From Martins et al. (2025). This image is made available under a CC-BY 4.0 International license.

Cell signalling, migration and mechanosensing

ERM proteins support perinuclear actin rim formation

Andrea Fracchia, Yuval Hadad, Dagmawit Babele, Gabi Gerlitz

https://www.biorxiv.org/content/10.1101/2025.02.22.639617v1

This preprint investigates how ERM proteins link actin filaments to the nuclear envelope, which is important for cell migration, nuclear positioning, and transcriptional control. The study finds that ERM proteins localize to the nuclear envelope and support the formation of the perinuclear actin rim in melanoma cells.

Picked by: Felipe Del Valle Batalla

Axonal Mechanotransduction Drives Cytoskeletal Responses to Physiological Mechanical Forces

Grace L Swaim, Oliver V. Glomb, Yi Xie, Chloe Emerson, Zhuoyuan Li, Daniel Beaudet, Adam G. Hendricks, Shaul Yogev

https://www.biorxiv.org/content/10.1101/2025.02.11.637689v1

This preprint highlights the role of the mechanosensitive protein Talin, the small GTPase RhoA, and actomyosin activity in the axon to maintain its integrity in the face of tension due to physical movements (muscle contractions). Also, in vivo live imaging of microtubules at single polymer resolution!

Picked by: Girish Kale

Stiffening cells with light

Eva Gonzalez, Jana El Husseiny, Finn Bastian Molzahn, Tiffany Campion, Hadrien Jalaber, Stéphanie Dogniaux, Pierre-Henri Puech, Oliver Nüsse, Laure Gibot, Julien Husson

https://www.biorxiv.org/content/10.1101/2025.01.30.635790v1.abstract?%3Fcollection=

This study reveals a direct relationship between fluorescence excitation and cell mechanical properties, highlighting that the stiffness of T cells can be rapidly increased by exposing to excitation light. Also propose “photostiffening” as a new method for rapidly quantifying phototoxicity.

Picked by:

From Gonzalez et al. (2025). This image is made available under a CC-BY 4.0 International license.

Ca2+ increases cardiac muscle viscoelasticity independent of active force development

Anthony J. Baker, On Yeung Li, Filip Ježek, Paul C. Simpson, Naomi C. Chesler, Daniel A. Beard

https://www.biorxiv.org/content/10.1101/2025.02.26.640328v1

This preprint looks at a secondary role of Ca2+ in increasing the viscoelasticity of cardiac muscles.

Picked by: Girish Kale

Dynamic and Biphasic Regulation of Cell Migration by Ras

Yiyan Lin, Eleana Parajón, Qinling Yuan, Siyu Ye, Guanghui Qin, Yu Deng, Jane Borleis, Ariel Koyfman, Pablo A. Iglesias, Konstantinos Konstantopoulos, Douglas N. Robinson, Peter N. Devreotes

https://www.biorxiv.org/content/10.1101/2025.02.13.638204v1.full

This preprint identifies how Ras GTPase activity biphasically regulates cell migration, with excessive and insufficient activity impairing movement. This finding highlights the need for nuanced therapeutic targeting in cancer treatment.

Picked by: Vibha Singh

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Roberto Amadio

Biochemistry

Enzymatic access to the rare ΔUA (1→4) Glc 3, 6, N-sulfated heparin disaccharide, implications for heparin quality control

T. K. Kandola, C. J. Mycroft-West, M. L. Andrade De Lima, et al.

Selected by 03 January 2025

Adriani Felix et al.

Biochemistry

Factor XI localization in human deep venous thrombus and function of activated factor XI on venous thrombus formation and hemostasis in rabbit

Nobuyuki Oguri, Toshihiro Gi, Eriko Nakamura, et al.

Selected by 03 January 2025

Adriani Felix et al.

Biochemistry

Also in the biophysics category:

Target cell tension regulates macrophage trogocytosis

Caitlin E. Cornell, Aymeric Chorlay, Deepak Krishnamurthy, et al.

Selected by 04 January 2025

Felipe Del Valle Batalla

Biophysics

Restoring mechanophenotype reverts malignant properties of ECM-enriched vocal fold cancer

Jasmin Kaivola, Karolina Punovuori, Megan R. Chastney, et al.

Selected by 19 December 2024

Teodora Piskova

Cancer Biology

Motor Clustering Enhances Kinesin-driven Vesicle Transport

Rui Jiang, Qingzhou Feng, Daguan Nong, et al.

Selected by 16 November 2024

Sharvari Pitke

Biophysics

Also in the cancer biology category:

Target cell tension regulates macrophage trogocytosis

Caitlin E. Cornell, Aymeric Chorlay, Deepak Krishnamurthy, et al.

Selected by 04 January 2025

Felipe Del Valle Batalla

Biophysics

Restoring mechanophenotype reverts malignant properties of ECM-enriched vocal fold cancer

Jasmin Kaivola, Karolina Punovuori, Megan R. Chastney, et al.

Selected by 19 December 2024

Teodora Piskova

Cancer Biology

Integrin conformation-dependent neutrophil slowing obstructs the capillaries of the pre-metastatic lung in a model of breast cancer

Frédéric Fercoq, Gemma S. Cairns, Marco De Donatis, et al.

Selected by 07 October 2024

Simon Cleary

Cancer Biology

Also in the cell biology category:

Deletion of PIEZO1 in adult cardiomyocytes accelerates cardiac aging and causes premature death

Ze-Yan Yu, Yang Guo, Scott Kesteven, et al.

Selected by 17 March 2025

Theodora Stougiannou

Cell Biology

Megakaryocytes assemble a three-dimensional cage of extracellular matrix that controls their maturation and anchoring to the vascular niche

Claire Masson, Cyril Scandola, Jean-Yves Rinckel, et al.

Selected by 21 February 2025

Simon Cleary

Physiology

Fis1 is required for the development of the dendritic mitochondrial network in pyramidal cortical neurons

Klaudia Strucinska, Parker Kneis, Travis Pennington, et al.

Selected by 11 February 2025

Felipe Del Valle Batalla

Cell Biology

Also in the molecular biology category:

Conservation and divergence of regulatory architecture in nitrate-responsive plant gene circuits

C Bian, GS Demirer, MT Oz, et al.

Selected by 14 March 2025

Jeny Jose

Plant Biology

Levetiracetam prevents Aβ42 production through SV2a-dependent modulation of App processing in Alzheimer’s disease models

Nalini R. Rao, Olivia DeGulis, Toshihiro Nomura, et al.

Selected by 11 January 2025

Jawdat Sandakly

Neuroscience

Chromosomal instability in human trophoblast stem cells and placentas

Danyang Wang, Andrew Cearlock, Katherine Lane, et al.

Selected by 31 December 2024

Carly Guiltinan

Cell Biology