ASCB/EMBO Annual Meeting 2018
Partially redundant actin genes in Chlamydomonas control flagellum-directed traffic and transition zone organization.
Highly divergent, unconvential actin Nap1 plays a key role in multiple steps of Chlamydomonas flagellar biosynthesis.
Evolution and Engineering of Allosteric Regulation in Protein Kinases
How does new allosteric regulation evolve? Mutational scanning of kinase sectors reveals hotspots that can be used to rewire signaling networks.
Nano-scale size holes in ER sheets provide an alternative to tubules for highly-curved membranes
STED super-resolution imaging reveals sub-diffraction holes in ER sheets, regulated by Climp63 and serving as reservoirs for curvature-sensing proteins.
Expression of TorsinA in a heterologous yeast system reveals interactions with conserved lumenal domains of LINC and nuclear pore complexes
Despite yeast not having a Torsin homolog, heterologously-expressed atypical AAA-ATPase Torsin A reveals conserved interactions with SUN-domain proteins at SPBs and the Pom152 component of the NPC.
Ultra-fast cellular contractions in the epithelium of T. adhaerens and the “active cohesion” hypothesis
Trichoplax is only composed of two epithelial layers, yet is capable of ultra-fast contractions mediated by non-muscle acto-myosin networks in an actively-softening tissue.
Stem cell differentiation trajectories in Hydra resolved at single-cell resolution
Transcriptome sequencing produces the first comprehensive map of the developmental trajectory for each lineage in the adult Hydra polyp.
Deep convolutional and recurrent neural networks for cell motility discrimination and prediction
Using CNNs to classify and predict muscle stem cell motility behaviors.
An amphipathic helix enables septins to sense micron-scale membrane curvature
Although septins polymerize on membranes cooperatively, a single amphipathic helix is sufficient to detect curvature.
Reconstitution of the equilibrium state of dynamic actin networks
Investigating dynamic actin networks in silico and in vitro reveals a key role for ADF/Cofilin
Activation of SEDS-PBP cell wall synthases by an essential regulator of bacterial division
The Caulobacter protein FzlA links FtsZ to cell wall peptidoglycan synthesis during cell division, serving both as activator and spatial cue.
Dynamic multifactor hubs interact transiently with sites of active transcription in Drosophila embryos
Lattice light sheet imaging of Zelda and Bicoid reveal the formation of unexpectedly short-lived transcription factor hubs at sites of active transcription.
Dynamic and Selective Low-Complexity Domain Interactions Revealed by Live-Cell Single-Molecule Imaging
Many transcription factors contain low-complexity domains (LCDs). These LCD-LCD interactions might involve liquid-liquid phase separation and play a role in regulating interactions between transcription factors and PolII.
Cryo-ET reveals nucleosome reorganisation in condensed mitotic chromosomes in vivo
Nucleosomes in mitotic fission yeast chromosomes are organised into larger clusters than in interphase, but the overall clustering density remains the same.
Mammalian kinetochores count attached microtubules in a sensitive and switch-like manner to control cell cycle progression
Detaching individual microtubules from the spindle, and independently tuning Hec1-microtubule binding activity, shows that the spindle assembly checkpoint responds not to force but to the number of microtubule-kinetochore contacts.
Reversible solidification of fission yeast cytoplasm after prolonged nutrient starvation
A cytoplasmic mesh immobilizes organelles and other components of the fission yeast cytoplasm after multiple days of nutrient starvation.
A synthetic biology platform for the reconstitution and mechanistic dissection of LINC complex assembly
Artificial nuclear membranes can support SUN-KASH interactions, revealing SUN transmembrane topology and providing a synthetic platform for understanding LINC biology.
Contractile actomyosin network on nuclear envelope remnants positions chromosomes for mitosis
LINC-dependent actomyosin contraction prevents chromosomes from scattering after nuclear envelope breakdown.
Regulation of Cilia Abundance in Multiciliated Cells
Cell surface area directly regulates the abundance of cilia in ex-vivo multiciliated airway culture.
Superresolution architecture of pluripotency guarding adhesions
Physical restriction of adhesion size in hPSC colonies can force exit from pluripotency; these “cornerstone” focal adhesions have an unusual 3D architecture, revealed by super-resolution imaging.
Intravascular arrest of circulating tumor cells is a two-step process exploiting their adhesion repertoire
Using intravital imaging in zebrafish the multistep process of adhesion and subsequent extravasation of circulating tumor cells can be determined in detail.
A novel interplay between GEFs orchestrates Cdc42 activity during cell polarity and cytokinesis
The crosstalk between GEFs permits the spatial and temporal regulation of Cdc42 activity
Also in the cell biology category:
EMBL Seeing is Believing – Imaging the Molecular Processes of Life
Preprints discussed at the 2019 edition of Seeing is Believing, at EMBL Heidelberg from the 9th-12th October 2019
|List by||Gautam Dey|
Preprints on autophagy and lysosomal degradation and its role in neurodegeneration and disease. Includes molecular mechanisms, upstream signalling and regulation as well as studies on pharmaceutical interventions to upregulate the process.
|List by||Sandra Malmgren Hill|
Lung Disease and Regeneration
This preprint list compiles highlights from the field of lung biology.
|List by||Rob Hynds|
A curated list of preprints related to cellular metabolism at Biorxiv by Pablo Ranea Robles from the Prelights community. Special interest on lipid metabolism, peroxisomes and mitochondria.
|List by||Pablo Ranea Robles|
BSCB/BSDB Annual Meeting 2019
Preprints presented at the BSCB/BSDB Annual Meeting 2019
|List by||Gautam Dey|
This list of preprints is focused on work expanding our knowledge on mitochondria in any organism, tissue or cell type, from the normal biology to the pathology.
|List by||Sandra Franco Iborra|
Biophysical Society Annual Meeting 2019
Few of the preprints that were discussed in the recent BPS annual meeting at Baltimore, USA
|List by||Joseph Jose Thottacherry|
Also in the cell biology category:
Mitochondrial RNA granules are fluid condensates, positioned by membrane dynamics
|Selected by||Romain F. Laine|
Resolving kinesin stepping: one head at a time
|Selected by||Ben Craske, Gaetan Dias Mirandela, Thibault Legal and Toni McHugh|
Mammalian Y RNAs are modified at discrete guanosine residues with N-glycans
|Selected by||Connor Rosen|