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Zebrafish immunology

A compilation of cutting-edge research that uses the zebrafish as a model system to elucidate novel immunological mechanisms in health and disease.

List by Shikha Nayar

Preprints:

Dynamic control of proinflammatory cytokines Il-1β and Tnf-α by macrophages is necessary for functional spinal cord regeneration in zebrafish

Themistoklis M. Tsarouchas, Daniel Wehner, Leonardo Cavone, Tahimina Munir, Marcus Keatinge, Marvin Lambertus, Anna Underhill, Thomas Barrett, Elias Kassapis, Nikolay Ogryzko, Yi Feng, Tjakko J. van Ham, Thomas Becker, Catherina G. Becker,

https://www.biorxiv.org/content/10.1101/332197v2

This study importantly addresses and dissects the dynamics of cytokine production through injury and development, to show that appropriate and timely induction of pro-inflammatory cytokines can in fact play a key role in repair.

RNAseq profiling of leukocyte populations in zebrafish larvae reveals a cxcl11 chemokine gene as a marker of macrophage polarization during mycobacterial infection.

Julien Rougeot, Vincenzo Torraca, Ania Zakrzewska, Zakia Kanwal, Hans Jansen, Herman P Spaink, Annemarie H Meijer

https://www.biorxiv.org/content/10.1101/554808v1

This study focusses on understanding the differences in key leukocyte populations in the zebrafish under mycobacterial infection. Importantly, the authors extrapolate transcriptomic analyses to human macrophage subsets, validating the use of the zebrafish as a model organism to study such populations at a granular level.

Hif-1α stabilisation polarises macrophages via cyclooxygenase/prostaglandin E2 in vivo

Amy Lewis, Philip Michael Elks

https://www.biorxiv.org/content/10.1101/536862v2

This paper importantly links microenvironmental cues to immunological subsets in the zebrafish to underscore these interactions in inflammation, cancer, and infection. Specifically, authors dissect the effect of HIF1a on macrophage polarization via PGE2.

The CXCL12/CXCR4 signalling axis retains neutrophils at inflammatory sites in zebrafish

Hannah M. Isles, Kimberly Herman, Anne L. Robertson, Catherine A. Loynes, Lynne R. Prince, Philip M. Elks, Stephen A. Renshaw

https://www.biorxiv.org/content/10.1101/626978v1

This study interrogates how the CXCL12/CXCR4 axis recruits and maintains neutrophil populations at wound sites under infections. These findings can help pinpoint novel targets for therapeutic purposes in these chronic inflammatory conditions.

Live imaging the Foreign Body Response reveals how dampening inflammation reduces fibrosis

David B. Gurevich, Kathryn E. French, John D. Collin, Stephen J. Cross, Paul Martin

https://www.biorxiv.org/content/10.1101/498444v2

This study uses the powerful means of live imaging in the zebrafish to visualize inflammation and fibrosis in a foreign body response model. The careful visualization allows for interrogation of responses that might be impaired in similar processes in humans.

Thrombocyte inhibition restores protective immunity to mycobacterial infection in zebrafish

Elinor Hortle, Khelsey E. Johnson, Matt D. Johansen, Tuong Nguyen, Jordan A. Shavit, Warwick J. Britton, David M. Tobin, Stefan H. Oehlers

https://www.biorxiv.org/content/10.1101/338111v4

This study interrogates interactions between thrombocytes and macrophages in Tb infection. Importantly, authors investigate why these interactions are important both for maintaining homeostasis, and also under cases of infection.

Lipid Droplet metabolism dependent microbial defense in pre-immune zebrafish embryos

Asmita Dutta, Sampali Banerjee, Deepak Kumar Sinha

https://www.biorxiv.org/content/10.1101/478859v1

This study takes advantage of placing zebrafish embryos in a microbe-free environment to interrogate how embryos release pro-microbial compounds into the environment. Specifically, they investigate how these secretions help stimulate/balance lipid secretion to maintain homeostasis.

Single-cell transcriptional analysis reveals naïve helper ILC-like cells in zebrafish

Pedro P. Hernández, Paulina M. Strzelecka, Emmanouil I. Athanasiadis, Ana F. Robalo, Catherine M. Collins, Pierre Boudinot, Jean-Pierre Levraud, Ana Cvejic

https://www.biorxiv.org/content/10.1101/342477v1

This study utilizes single-cell transcriptomics to identify novel ILC-like populations in the zebrafish. Importantly, this will deepen our understanding in how this fairly newly identified cellular population functions following activation and infection.

Non-apoptotic pioneer neutrophils initiate an endogenous swarming response in a zebrafish tissue injury model

Hannah M. Isles, Clare F. Muir, Noémie Hamilton, Anastasia Kadochnikova, Catherine A. Loynes, Visakan Kadirkamanathan, Philip M. Elks, Stephen A. Renshaw

https://www.biorxiv.org/content/10.1101/521450v2

This study aims to understand dynamics of neutrophil swarming at inflammatory sites. Importantly, authors were able to identify pioneer neutrophil subsets that prime further migration at a wound site, giving us insight into endogenous neutrophil migration patterns.

Human macrophages survive and adopt activated genotypes in living zebrafish

Colin D Paul, Alexus Devine, Kevin Bishop, Qing Xu, William J Wulftange, Hannah Burr, Kathryn M Daly, Chaunte Lewis, Daniel S Green, Jack R Staunton, Swati Choksi, Zheng-Gang Liu, Raman Sood, Kandice Tanner

https://www.biorxiv.org/content/10.1101/181685v3

This study successfully uses human monocyte/macrophage engraftment in the zebrafish to better understand these immune dynamics in vivo. Authors were able to identify key transcripts and protein that represent an activated population of macrophages relevant to those seen in cancer progression.

High cholesterol diet modulates macrophage polarization and liver inflammation during early hepatocellular carcinoma progression in zebrafish

Sofia de Oliveira, Ruth A. Houseright, Alyssa L. Graves, Netta Golenberg, Benjamin G. Korte, Veronika Miskolci, Anna Huttenlocher

https://www.biorxiv.org/content/10.1101/299016v1

In this study, authors take advantage of transparent optics of zebrafish to visualize liver inflammation in real-time in a NAFLD/NASH-HCC model. They specifically interrogate the effect of a high fat diet on macrophage polarization in this model, and find that a high fat diet exacerbates disease.

Categories: developmental biology , genetics , genomics , immunology

 

Posted on: 20th May 2019

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