ATM-dependent formation of a novel chromatin compartment regulates the Response to DNA Double Strand Breaks and the biogenesis of translocations
Preprint posted on 8 November 2021 https://www.biorxiv.org/content/10.1101/2021.11.07.467654v1
The physical arrangement of chromatin within the nuclear space plays an important role in mediating many DNA-associated processes. For example, gene expression regulation, DNA duplication and DNA repair (1). Broadly, chromosomes are segregated into two ‘compartments’ known as ‘A’ (a gene dense and transcriptionally active) and ‘B’ (repressed chromatin). These regions are sub-divided into topologically associated domains (TADs), reflecting the level of contact between DNA regions. DNA regions within a TAD interact more frequently with each other than with DNA regions in other TADs. Within TADs the chromatin can physically ‘loop’ providing an additional structural layer (1).
We understand less about how chromatin organisation in the nucleus contributes to the cellular response to DNA injuries, which can drive instability in the genome.
Double strand breaks (DSBs) arise when both strands of the DNA helix break. If un-repaired, they can result in rearrangements, translocation events or the loss of DNA sequences thereby compromising the genome. At the heart of DSB repair are the kinases (proteins which transfer information to other proteins via phosphorylation), Ataxia-telangiectasia Mutated (ATM), and DNA protein kinase (DNA-PK). Both kinases are essential for orchestrating repair and preparing the chromatin environment by phosphorylating a variant histone H2AX (in mammals) to form yH2AX and subsequently recruiting other repair factors (2). Decades of work have characterised in detail DSB repair, though we are yet to understand why DSBs ‘cluster’ within the nucleus, forming sites of DNA repair, as this organisation could potentially promote more instability. For example, during the process of repair, DNA ends could be incorrectly joined if they are close together (3).
Here, the authors investigate how the chromatin structure alters in the presence of DSBs. Their work now supports the presence of a new G1-phase and DSB specific chromatin compartment (the ‘D’ compartment). They suggest that this compartment, which is associated with DSB clusters, could activate, and concentrate some DNA damage associated factors thereby generating an optimal repair response/environment to tackle DSBs. However, they show that such compartmentalisation may also account for some genome instability events seen in cancerous cell lines.
Here, the authors use a human cell line they developed called DIvA cells. DIvA (DSB Inducible via AsiSI) cells can be induced to produce ~ 100 DSBs using an endonuclease called AsiSI. Adding OHT (hydroxytamoxifen) induces the movement of the AsiSI endonuclease from cytoplasm to nucleus, where it then cuts the DNA (4). To assess changes in chromatin structure after a DSB, the authors performed chromatin conformation capture experiments including Hi-C and 4C-seq. Briefly, Hi-C preserves interactions between different regions of DNA via cross-links in an unbiased manner. Once the cross-linked DNA is digested, biotinylated nucleotides are incorporated at DNA ends. The fragments are then ligated together and after, the crosslinks are removed. This results in the formation of a hybrid DNA fragment containing DNA regions that are distant linearly in the genome but that were close physically in the nuclear space at the beginning of the experimental procedure. Using streptavidin (which binds biotin), these hybrid DNA fragments are isolated then analysed to measure the interaction frequency between these distant genomic loci (5). 4C-seq follows a similar concept but allows the detection of all potential sequences which are interacting with a specific DNA region of interest (6).
A) Damaged TADs are supported by ATM activity
Performing Hi-C in DIvA cells in the presence and absence of ATM kinase activity, revealed that if ATM activity was present, TADs containing DSBs were less in contact physically with other (non-DSB containing) TADs. In some cases, the DSB itself became isolated within the TAD. Whereas, inhibiting ATM activity in the presence of DSBs using an ATM-specific kinase inhibitor (KU-55933; 7), the authors found less interactions occurring within TADs and more limited spreading of yH2AX signal. They also noted that blocking ATM activity prevents DNA loop extrusion, which the authors previously demonstrated as a driver of DSB repair foci. Loop extrusion is a process by which chromatin ‘loops’ grows via the action of cohesin (8). In contrast, by inhibiting DNA-PK, which impair severely DSB repair, using a kinase inhibitor (NU-7441), they found increased intra-TAD interactions, accumulation of yH2AX and increased loop extrusion.
Thus, ATM activity appears to promote isolation of the DSB damaged region and can induce alterations to chromatin structure.
Figure shows a selection of figures from Arnould et al. Figure 1A shows Hi-C data from DIvA cells in the presence or absence of DSBs for part of chromosome 1 at three resolutions: 250 kb (left), 100 kb (middle) and 25 kb (right). The top tracks show the locations of yH2A signal indicating where a DSB has occurred. Red square (a damaged TAD with enhanced cis interactions), Blue square (decreased interaction between damaged TAD and nearby TAD. Figure 1E shows Hi-C contact matrices in the presence or absence of DSBs in control cells (left) and cells in which either DNA-PK (middle) or ATM (right) have been inhibited. Figure 2B shows Hi-C data in the presence or absence of DSBs on part of chromosome 17. The tracks on top and left show ChIP-seq defined locations of yH2AX and 53BP1. Black arrow = clustering of one DSB with several others on chromosome 17. Figure 2E shows Hi-C data as above (for a region on chromosome 1) but with the additional inhibition of either DNA-PK or ATM. Top tracks show yH2AX signal as defined by ChIP-seq. Boxes below show Hi-C between two yH2AX domains for each condition. Figure 4A shows qPCR data to assess translocation frequency for two different translocations after DSBs were induced in synchronised cells (as detailed on the graph). Figure 4H shows sites in which there are overlaps between sites of translocation events in cancer cells and genes associated with the D compartment. Expected overlap (grey, computationally defined), observed overlap (green). Figures adapted from Arnould et al., and made available under a CC-BY 4.0 International license.
B) DSB clustering generally happens during G1-phase of the cell cycle
The author’s data suggests that TADs containing a DSB can form close contacts (i.e cluster) with other DSB containing TADs. To find out what chromatin features were associated with these multiple TAD sites, they found yH2AX signal, other DSB associated chromatin marks (i.e loss of H1 histone, 53BP1 accumulation) and RNA polymerase II (RNAPII), all of which were determined via chromatin immunoprecipitation. Thus, DSB-associated/DNA repair factors are found at DSB clusters and these clustered DSBs came from sites of active transcription. By inhibiting ATM as before, the authors show that the clustering behaviour requires active ATM (but not DNA-PK whose inhibition enhanced the process). Lastly, the authors found that in synchronised cells (using thymidine which arrests cells at the G1/S transition), this clustering pattern appeared more prominently in G1 stage cells.
C) The ‘D’ compartment – a DSB clustering site
As mentioned, chromatin can be compartmentalised into ‘A’ (active) and ‘B’ (repressed) compartments. Though DSBs did not appear to affect this organisation nor did inhibiting ATM or DNA-PK, they saw that clustering was more common when a DSB happened within the ‘A’ compartment. Upon further investigation of their data, the authors uncovered an additional ‘A’ associated sub-compartment, referred to as the ‘D’ (DSB-induced) compartment. They found this sub-compartment to be rich in yH2AX signal however some TADs associated with this sub-compartment did not appear to be yH2AX containing but were instead enriched for other chromatin marks associated with active transcription.
D) The D compartment – a hub of DSB repair?
Some undamaged TADs are associated with the ‘D’ compartment but why is unclear. To understand this behaviour, the authors examine for both DNA motifs and performed RNA-seq analysis in the presence and absence of DSB induction to look for gene expression changes associated with the ‘D’ compartment. They found that some tumour suppressor genes and DNA damage response factors were up-regulated, and associated with the ‘D’ compartment. When they disrupted the cell’s ability to cluster DSBs (via RNAi depletion of a factor called SUN2), they found reduced up-regulation of these genes via qPCR. Specifically, they examined for change in PLK3 (polo-like kinase3), RNF19B (ring finger protein 19B) and GADD45A (growth arrest and DNA damage inducible alpha), all of which are associated with DNA repair/tumour suppression responses. Thus, recruitment of these genes to the ‘D’ compartment could aid activation.
E) DSB clustering can result in translocations
A key negative consequence of DSB clustering is the potential for chromosome translocations (where chromosome/break ends are joined incorrectly). To ask about the frequency of translocations after DSBs, the authors used qPCR and Amplicon-seq assays (amplification and sequencing of DSB ends). This allowed to test for intra-chromosomal (i.e happening on the same chromosome) and inter-chromosomal translocations (i.e between different chromosomes). In keeping with ‘D’ compartment predominance in G1, the authors found that more translocations were happening during this cell cycle stage. If the authors enhance ‘D’ compartment formation (by blocking DNA-PK) they find increased translocations, whereas disrupting DSB clustering reduced translocations, suggesting that DSB clustering can lead to both intra and inter-chromosomal translocations. Interestingly, by limiting loop extrusion actors, they found less intra-chromosomal translocations, suggesting loop extrusion also plays a role in these events.
As genome instability, including translocations, are common hallmarks of cancer cells, the authors also investigated if some translocations occurring in cancer cells could be associated with the clustering of DSBs and DNA damage response genes in the ‘D’ compartment. Taking a computational approach, they demonstrated a correlation between genes associated with the ‘D’ compartment following a DSB with points where translocations were observed in cancerous genomes indicating that some translocation events could be perpetuated by events occurring within the ‘D’ compartment.
What I liked about this preprint:
I really like this study as I think it demonstrates well the power of mining next generation sequence data sets to retrieve novel and exciting biology. Here the authors combine some of their prior observations and across the field to address a key gap in our understanding of DSB behaviour. Though more work is needed to really understand the fine aspects of the clustering process, the results here now open a new avenue of investigation.
- Szabo, F. Bantignies and G. Cavalli. Principles of genome folding into topologically associating domains. Science Advances. 2019.
- Scully, A. Panday, R. Elango and N.A. Willis. DNA double-strand break repair-pathway choice in somatic mammalian cells. Nat. Rev. Molecular Cell Biology. 2019.
- Marnef and G. Legube. Organizing DNA repair in the nucleus: DSBs hit the road. Current Opinion in Cell Biology. 2017.
- Kong and Y. Zhang. Deciphering Hi-C: from 3D genome to function. Cell Biology and Toxicology. 2019.
- H.L. Krijgerm G. Geeven, V. Bianchi, C.P.E. Hilvering and W. de Laat. 4C-seq from beginning to end: A detailed protocol for sample preparation and data analysis. Methods. 2020.
- Hickson, Y. Zhao, C.J. Richardson, S.J. Green, N.M. Martin, A.I. Orr et al. Identification and characterisation of a novel and specific inhibitor of the ataxia-telangiectasia mutated kinase ATM. Cancer Research. 2004.
- Arnould, V.Rocher, A-L. Finoux, T. Clouaire, K. Li, F. Zhou, P. Caron, P.lE. Mangeot, E.P. Ricci, R. Mourad, J.E. Haber, D. Noordermeer and G. Legube. Loop extrusion as a mechanism for formation of DNA damage repair foci. Nature. 2021.
- Aymard, M.Aguirrebengoa, E.Guillou, B.M.Javierre, B.Bugler, C.Arnould, V.Rocher, J.S.Iacovoni, A.Biernacka, M.Skrzypczak, K.Ginalski, M.Rowicka, P.Fraser and G.Legube. Genome-wide mapping of long-range contacts unveils clustering of DNA double-strand breaks at damaged active genes. Nature Structural & Molecular Biology. 2017
- Roukos, T.C.Voss, C.K.Schmidt, S.Lee, D.Wangsa and T.Misteli. Spatial dynamics of chromosome translocations in living cells. Science. 2013.
- Neumaier, J.Swenson, C.Pham, A.Polyzos, A.T.Lo, P.Yang, J.Dyball, A.Asaithamby, D.J.Chen, M.J.Bissell, S.Thalhammer and S.V.Costes. Evidence for formation of DNA repair centers and dose-response nonlinearity in human cells. PNAS. 2012
- R.Schrank, T.Aparicio, Y.Li, W.Chang, B.T.Chait, G.G.Gundersen, M.E.Gottesman and J.Gautier. Nuclear ARP2/3 drives DNA break clustering for homology-directed repair. Nature. 2018.
Posted on: 17 February 2022Read preprint
Also in the molecular biology category:
ARHGEF18 participates in Endothelial Cell Mechano-sensitivity in Response to Flow
|Selected by||Aarren Mannion|
Targeting a broad spectrum of KRAS-mutant cancers by hyperactivation-induced cell death
|Selected by||Yvonne Xinyi Lim|
Disrupting cellular memory to overcome drug resistance
|Selected by||Nate Mullin|
preListsmolecular biology category:in the
EMBL Synthetic Morphogenesis: From Gene Circuits to Tissue Architecture (2021)
A list of preprints mentioned at the #EESmorphoG virtual meeting in 2021.
|List by||Alex Eve|
A collection of preprints presented during the virtual meeting of the Federation of European Neuroscience Societies (FENS) in 2020
|List by||Ana Dorrego-Rivas|
ECFG15 – Fungal biology
Preprints presented at 15th European Conference on Fungal Genetics 17-20 February 2020 Rome
|List by||Hiral Shah|
ASCB EMBO Annual Meeting 2019
A collection of preprints presented at the 2019 ASCB EMBO Meeting in Washington, DC (December 7-11)
|List by||Madhuja Samaddar et al.|
Lung Disease and Regeneration
This preprint list compiles highlights from the field of lung biology.
|List by||Rob Hynds|
This list of preprints is focused on work expanding our knowledge on mitochondria in any organism, tissue or cell type, from the normal biology to the pathology.
|List by||Sandra Franco Iborra|