Close

Galleria mellonella as an Insect Model for P. destructans, the Cause of White-Nose Syndrome in Bats

Chapman N Beekman, Lauren Meckler, Eleanor Kim, Richard J Bennett

Posted on: 21 February 2018 , updated on: 22 February 2018

Preprint posted on 8 January 2018

Article now published in PLOS ONE at http://dx.doi.org/10.1371/journal.pone.0201915

White nose syndrome is decimating bat populations and now Chapman Beekman and colleagues are suggesting infecting greater wax moths (Galleria mellonella) with the fungus that causes the lethal infection to learn more about it.

Selected by Heath MacMillan
The experiments conducted by Chapman Beekman and Colleagues to test a new model of fungal infection. Graphical abstract graciously provided by the authors.

 

Context and major contributions:

White nose syndrome is responsible for killing millions of bats over the last decade, and continues to spread across Canada and the United States. Devising methods of control for the fungus responsible for the infection (Pseudogymnoascus destructans), however, requires a laboratory model for infection and the fungus grows slowly on bats in captivity. So why not try it on a fast growing and common insect model of infection? Chapman Beekman and colleagues first exposed wax moths (Galleria mellonella) to P. destructans, and monitored the animals for signs of infection. Indeed, moths exposed to spores were killed by the fungus in the days and weeks following infection, and germinated spores of killed moths at least three times faster than non-germinated spores. By contrast, heat killed spores had no effect on the moths, meaning infection and death from P. destructans requires live spores.

One of the main goals in studying P. destructans is to identify potent chemical inhibitors that may control or eliminate fungal infection in bats. The authors made an important contribution to this endeavor by identifying two fungicidal chemicals that facilitated moth survival in the presence of the fungus.

 

Outstanding questions:

  • Since white nose syndrome in mammals results from external, rather than internal infection (the moths were injected), are the mechanisms of infection similar enough for an insect to serve as a suitable model?
  • The physiological effects of destructans on bats (i.e. the mechanisms leading to death) are still under active investigation. Are these mechanisms likely to be shared across such distantly related animals?

 

Related Research:

Binder, U., Maurer, E., Lass-Florl C. (2016) Galleria mellonella: An invertebrate model to study pathogenicity in correctly defined fungal species. Fungal Biol 120:288-295.

McGuire, L.P., Mayberry, H. W., Willis, C. K. R. (2017) White-nose syndrome increases torpid metabolic rate and evaporative water loss in hibernating bats. Am J Physiol Regul Integr Comp Physiol 313: R680–R686.

Kryukov, V. Y., Yaroslavtseva, O. N., Whitten, M. M. A., Tyurin, M. V., Ficken, K. J., Greig, C., Melo, N. R., Glupov, V. V., Dubovskiy, I. M., Butt, T. M. (2017), Fungal infection dynamics in response to temperature in the lepidopteran insect Galleria mellonella. Insect Science DOI 10.1111/1744-7917.12426

 

 

 

Read preprint (No Ratings Yet)

Have your say

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

Sign up to customise the site to your preferences and to receive alerts

Register here

Also in the biochemistry category:

14-3-3 binding regulates Tau assembly and microtubule association

Janine Hochmair, Maxime C. M. van den Oetelaar, Lisa Diez, et al.

Selected by 03 May 2024

Barbora Knotkova et al.

Biochemistry

Structural basis of respiratory complexes adaptation to cold temperatures

Young-Cheul Shin, Pedro Latorre-Muro, Amina Djurabekova, et al.

Selected by 10 April 2024

Pamela Ornelas

Biophysics

Lens Placode Modulates Extracellular Matrix Formation During Early Eye Development

Cecília G. De Magalhães, Ales Cvekl, Ruy G. Jaeger, et al.

Selected by 28 February 2024

Bhaval Parmar

Developmental Biology

Also in the epidemiology category:

Multimodal interactions in Stomoxys navigation reveals synergy between olfaction and vision

Merid N Getahun, Steve Baleba, John Ngiela, et al.

Selected by 06 February 2024

Maitri Manjunath

Animal Behavior and Cognition

Mapping current and future thermal limits to suitability for malaria transmission by the invasive mosquito Anopheles stephensi

Sadie J. Ryan, Catherine A. Lippi, Oswaldo C. Villena, et al.

Selected by 02 January 2023

Sophia Friesen

Epidemiology

Isolation disrupts social interactions and destabilizes brain development in bumblebees

Z Yan Wang, Grace C. McKenzie-Smith, Weijie Liu, et al.

Selected by 13 January 2022

Niveda Udaykumar

Animal Behavior and Cognition

Also in the immunology category:

Fetal brain response to maternal inflammation requires microglia

Bridget Elaine LaMonica Ostrem, Nuria Dominguez Iturza, Jeffrey Stogsdill, et al.

Selected by 24 April 2024

Manuel Lessi

Neuroscience

Alteration of long and short-term hematopoietic stem cell ratio causes myeloid-biased hematopoiesis

Katsuyuki Nishi, Taro Sakamaki, Akiomi Nagasaka, et al.

Selected by 24 April 2024

Manuel Vicente, Anaisa Ferreira

Immunology

RIPK3 coordinates RHIM domain-dependent inflammatory transcription in neurons

Sigal B. Kofman, Lan H. Chu, Joshua M. Ames, et al.

Selected by 08 April 2024

Zoie Magri

Immunology

Also in the physiology category:

Blue appendages and temperature acclimation increase survival during acute heat stress in the upside-down jellyfish, Cassiopea xamachana

Megan E. Maloney, Katherine M. Buckley, Marie E. Strader

Selected by 30 April 2024

Maitri Manjunath

Animal Behavior and Cognition

How the liver contributes to stomach warming in the endothermic white shark Carcharodon carcharias

David C. Bernvi, Geremy Cliff

Selected by 22 April 2024

Sarah Young-Veenstra

Physiology

Unlocking the secrets of kangaroo locomotor energetics: Postural adaptations underpin increased tendon stress in hopping kangaroos

Lauren H. Thornton, Taylor J.M. Dick, John R. Hutchinson, et al.

Selected by 25 March 2024

EMB EMB_Liv et al.

Zoology

Also in the zoology category:

Blue appendages and temperature acclimation increase survival during acute heat stress in the upside-down jellyfish, Cassiopea xamachana

Megan E. Maloney, Katherine M. Buckley, Marie E. Strader

Selected by 30 April 2024

Maitri Manjunath

Animal Behavior and Cognition

How the liver contributes to stomach warming in the endothermic white shark Carcharodon carcharias

David C. Bernvi, Geremy Cliff

Selected by 22 April 2024

Sarah Young-Veenstra

Physiology

Multimodal interactions in Stomoxys navigation reveals synergy between olfaction and vision

Merid N Getahun, Steve Baleba, John Ngiela, et al.

Selected by 06 February 2024

Maitri Manjunath

Animal Behavior and Cognition

preLists in the biochemistry category:

BSCB-Biochemical Society 2024 Cell Migration meeting

This preList features preprints that were discussed and presented during the BSCB-Biochemical Society 2024 Cell Migration meeting in Birmingham, UK in April 2024. Kindly put together by Sara Morais da Silva, Reviews Editor at Journal of Cell Science.

 



List by Reinier Prosee

Preprint Peer Review – Biochemistry Course at UFRJ, Brazil

Communication of scientific knowledge has changed dramatically in recent decades and the public perception of scientific discoveries depends on the peer review process of articles published in scientific journals. Preprints are key vehicles for the dissemination of scientific discoveries, but they are still not properly recognized by the scientific community since peer review is very limited. On the other hand, peer review is very heterogeneous and a fundamental aspect to improve it is to train young scientists on how to think critically and how to evaluate scientific knowledge in a professional way. Thus, this course aims to: i) train students on how to perform peer review of scientific manuscripts in a professional manner; ii) develop students' critical thinking; iii) contribute to the appreciation of preprints as important vehicles for the dissemination of scientific knowledge without restrictions; iv) contribute to the development of students' curricula, as their opinions will be published and indexed on the preLights platform. The evaluations will be based on qualitative analyses of the oral presentations of preprints in the field of biochemistry deposited in the bioRxiv server, of the critical reports written by the students, as well as of the participation of the students during the preprints discussions.

 



List by Marcus Oliveira

CellBio 2022 – An ASCB/EMBO Meeting

This preLists features preprints that were discussed and presented during the CellBio 2022 meeting in Washington, DC in December 2022.

 



List by Nadja Hümpfer et al.

20th “Genetics Workshops in Hungary”, Szeged (25th, September)

In this annual conference, Hungarian geneticists, biochemists and biotechnologists presented their works. Link: http://group.szbk.u-szeged.hu/minikonf/archive/prg2021.pdf

 



List by Nándor Lipták

Fibroblasts

The advances in fibroblast biology preList explores the recent discoveries and preprints of the fibroblast world. Get ready to immerse yourself with this list created for fibroblasts aficionados and lovers, and beyond. Here, my goal is to include preprints of fibroblast biology, heterogeneity, fate, extracellular matrix, behavior, topography, single-cell atlases, spatial transcriptomics, and their matrix!

 



List by Osvaldo Contreras

ASCB EMBO Annual Meeting 2019

A collection of preprints presented at the 2019 ASCB EMBO Meeting in Washington, DC (December 7-11)

 



List by Madhuja Samaddar et al.

EMBL Seeing is Believing – Imaging the Molecular Processes of Life

Preprints discussed at the 2019 edition of Seeing is Believing, at EMBL Heidelberg from the 9th-12th October 2019

 



List by Dey Lab

Cellular metabolism

A curated list of preprints related to cellular metabolism at Biorxiv by Pablo Ranea Robles from the Prelights community. Special interest on lipid metabolism, peroxisomes and mitochondria.

 



List by Pablo Ranea Robles

MitoList

This list of preprints is focused on work expanding our knowledge on mitochondria in any organism, tissue or cell type, from the normal biology to the pathology.

 



List by Sandra Franco Iborra
Close