Close

Lineage tracing axial progenitors using Nkx1.2CreERT2 mice defines their trunk and tail contributions

Aida Rodrigo Albors, Pamela A Halley, Kate G Storey

Preprint posted on 7 February 2018 https://www.biorxiv.org/content/early/2018/02/07/261883

Article now published in Development at http://dx.doi.org/10.1242/dev.164319

Nkx1.2 is characterised as a new marker for neuro-mesodermal progenitors in the gastrulating mouse embryo.

Selected by Pierre Osteil

Categories: developmental biology

Background:

During mouse embryonic development, cells undergo a series of transformations. One of them is the formation of the paraxial mesoderm and neural tube, both thought to originate from the same cells, called Neuro-Mesodermal Progenitors (NMPs – for a more detailed introduction to NMPs you can refer to my previous highlight 1). NMPs are transient and very rare in the embryo, complicating efforts to study their development. Recently, two reports deciphered the transcriptomic signature of NMPs in the embryo 2,3. They identified these cells by the dual expression of Sox2 (a neural marker) and T (a mesoderm marker). From then, they identified unique transcription factors (TFs) expressed in these cells, such as Nkx1.2 Cdx2 and Cdx4 at E8.5, plus Tbx6 at E9.5. In their new preprint, Albors and colleagues extend this work, providing a comprehensive characterisation of the expression pattern of Nkx1.2 (a homeobox transcription factor), and identified Nkx1.2+ progeny cells by lineage tracking.

 

The data

First, in situ hybridization (ISH) was performed on Nkx1.2 in the mouse embryo from E6.0 to E12.5. Expression is first detected in E7.5 embryos in the posterior epiblast near where NMPs originate and is later seen in the neural tube and surrounding mesenchyme. Its expression also colocalised with Sox2+ and T+ regions. Following the characterisation of its expression pattern, they tagged Nkx1.2 with a CreERT2 cassette, then crossed it with R26R-EYFP (loxP flanked YFP) in order to permanently label progenies of all Nkx2.1+ cells. Tamoxifen was added at E7.5 and progeny cells were tracked at several stages. Nkx1.2+ cells at E7.5 are specialising into neurectoderm, several mesoderm tissues (excluding notochord) and, surprisingly, a few hindgut cells. These progenitors were maintained until E10.5 but no longer contributed to hindgut and surface ectoderm, rather labelling mostly trunk and tail tissues originating from the tail bud.

 

Why did I choose this article?

I was particularly interested by the lineage tracing experiments: using CreERT2 is a great way to understand TF dynamics as one can control the time of labelling. The authors have also produced compelling imaging data. They provided a thorough characterisation of a new marker that will allow the scientific community to perform deeper studies on NMPs.

Image reproduced from Albors et al., 2018 Fig 5B and 5Bd (section d)

 

Open questions

  • I wonder if the authors plan to give Tamoxifen at earlierstages: this could potentially identify younger progenitor cells? ISH might not be sensitive enough to detect low transcript levels.
  • Adding to that comment, Nkx1.2+ progeny cells were found in the hindgut (endoderm). So, it indicates that Nkx1.2 is expressed in a population that give rise to the three germ layers. Do the authors think that Nkx1.2 is actually labelling a pluripotent progenitor population?
  • Is Nkx1.2 expressed in in vitro-derived NMPs? Transcriptomic comparisons between in vitro and in vivo populations would be something to look forward to, as well as ChIP-sequencing to unveil its targets.

 

Related research:

  1. Edri, S., Hayward, P., Baillie-johnson, P. & Steventon, B. An Epiblast Stem Cell derived multipotent progenitor population for axial extension. (2018). doi:10.1101/242461
  2. Gouti, M. et al. A Gene Regulatory Network Balances Neural and Mesoderm Specification during Vertebrate Trunk Development. Dev Cell 41, 243–261.e7 (2017).
  3. Koch, F. et al. Antagonistic Activities of Sox2 and Brachyury Control the Fate Choice of Neuro-Mesodermal Progenitors. Dev Cell 42, 514–526.e7 (2017).

Tags: lineage tracing, mouse, nkx1.2, nmp

Posted on: 8 March 2018

Read preprint (No Ratings Yet)

Have your say

Your email address will not be published.

This site uses Akismet to reduce spam. Learn how your comment data is processed.

Sign up to customise the site to your preferences and to receive alerts

Register here

preLists in the developmental biology category:

2nd Conference of the Visegrád Group Society for Developmental Biology

Preprints from the 2nd Conference of the Visegrád Group Society for Developmental Biology (2-5 September, 2021, Szeged, Hungary)

 



List by Nándor Lipták

Fibroblasts

The advances in fibroblast biology preList explores the recent discoveries and preprints of the fibroblast world. Get ready to immerse yourself with this list created for fibroblasts aficionados and lovers, and beyond. Here, my goal is to include preprints of fibroblast biology, heterogeneity, fate, extracellular matrix, behavior, topography, single-cell atlases, spatial transcriptomics, and their matrix!

 



List by Osvaldo Contreras

EMBL Synthetic Morphogenesis: From Gene Circuits to Tissue Architecture (2021)

A list of preprints mentioned at the #EESmorphoG virtual meeting in 2021.

 



List by Alex Eve

EMBL Conference: From functional genomics to systems biology

Preprints presented at the virtual EMBL conference "from functional genomics and systems biology", 16-19 November 2020

 



List by Jesus Victorino

Single Cell Biology 2020

A list of preprints mentioned at the Wellcome Genome Campus Single Cell Biology 2020 meeting.

 



List by Alex Eve

Society for Developmental Biology 79th Annual Meeting

Preprints at SDB 2020

 



List by Irepan Salvador-Martinez, Martin Estermann

FENS 2020

A collection of preprints presented during the virtual meeting of the Federation of European Neuroscience Societies (FENS) in 2020

 



List by Ana Dorrego-Rivas

Planar Cell Polarity – PCP

This preList contains preprints about the latest findings on Planar Cell Polarity (PCP) in various model organisms at the molecular, cellular and tissue levels.

 



List by Ana Dorrego-Rivas

Cell Polarity

Recent research from the field of cell polarity is summarized in this list of preprints. It comprises of studies focusing on various forms of cell polarity ranging from epithelial polarity, planar cell polarity to front-to-rear polarity.

 



List by Yamini Ravichandran

TAGC 2020

Preprints recently presented at the virtual Allied Genetics Conference, April 22-26, 2020. #TAGC20

 



List by Maiko Kitaoka et al.

3D Gastruloids

A curated list of preprints related to Gastruloids (in vitro models of early development obtained by 3D aggregation of embryonic cells). Updated until July 2021.

 



List by Paul Gerald L. Sanchez and Stefano Vianello

ASCB EMBO Annual Meeting 2019

A collection of preprints presented at the 2019 ASCB EMBO Meeting in Washington, DC (December 7-11)

 



List by Madhuja Samaddar et al.

EDBC Alicante 2019

Preprints presented at the European Developmental Biology Congress (EDBC) in Alicante, October 23-26 2019.

 



List by Sergio Menchero et al.

EMBL Seeing is Believing – Imaging the Molecular Processes of Life

Preprints discussed at the 2019 edition of Seeing is Believing, at EMBL Heidelberg from the 9th-12th October 2019

 



List by Dey Lab

SDB 78th Annual Meeting 2019

A curation of the preprints presented at the SDB meeting in Boston, July 26-30 2019. The preList will be updated throughout the duration of the meeting.

 



List by Alex Eve

Lung Disease and Regeneration

This preprint list compiles highlights from the field of lung biology.

 



List by Rob Hynds

Young Embryologist Network Conference 2019

Preprints presented at the Young Embryologist Network 2019 conference, 13 May, The Francis Crick Institute, London

 



List by Alex Eve

Pattern formation during development

The aim of this preList is to integrate results about the mechanisms that govern patterning during development, from genes implicated in the processes to theoritical models of pattern formation in nature.

 



List by Alexa Sadier

BSCB/BSDB Annual Meeting 2019

Preprints presented at the BSCB/BSDB Annual Meeting 2019

 



List by Dey Lab

Zebrafish immunology

A compilation of cutting-edge research that uses the zebrafish as a model system to elucidate novel immunological mechanisms in health and disease.

 



List by Shikha Nayar
Close