Deterministic and probabilistic fate decisions co-exist in a single retinal lineage
Posted on: 15 November 2022 , updated on: 22 August 2023
Preprint posted on 11 August 2022
Article now published in The EMBO Journal at http://dx.doi.org/10.15252/embj.2022112657
To be or not to be a photoreceptor: cell fate choices in the developing zebrafish retina are deterministic and probabilistic.
Selected by Laura CelottoCategories: developmental biology, neuroscience
Updated 22 August 2023 with a postLight by Laura Celotto
“The EMBO Journal” has now published the insightful work below, which even made the cover of the journal last 17 July 2023. On the cover, you can appreciate the beauty of the zebrafish retina, which looks like a carpet of well-ordered layers, each of them hosting specific subsets of cells.
In the published paper, the authors have added two experiments that expand the findings of the preprint, without changing its main conclusions.
The first experiment attempts to answer one of the questions I also raise in my highlight on the preprint: How do you explain the thickness reduction of the ganglion cell layer upon Prdm1a knock down? As a brief recap, Prdmn1a is a transcription factor that determines photoreceptor fate in the developing zebrafish retina. In the preprint, the authors could show that injection of Prdmn1a morpholinos, which knock down the protein, decreased the thickness of not only the photoreceptor layer, but also of the ganglion cell layer.
In a new experiment of the published paper, the authors looked for signs of apoptosis in controls as well as Prdmn1a-injected zebrafish to check whether the injection of Prdmn1a morpholinos leads to unspecific cell death. Apoptosis is a form of programmed cell death, characterized by the activation of caspases, which are proteases – that is, proteins that “eat” other proteins. Among caspases, activated caspase-3 is considered the hallmark of apoptotic cells. The authors performed antibody staining against activated-caspase 3 in control as well as Prdmn1a knock down retinae. They observed few activated-caspase 3-positive cells in all layers of controls and Prdmn1a-knock down retinae, with no significant difference in the number of apoptotic cells between the two groups. Hence, they could conclude that apoptosis does not contribute to the thickness reduction of the photoreceptor and ganglion cell layers upon Prdmn1a knock down. Then, the authors speculated that Prdmn1a knock down could induce a decrease in the overall number of Atoh7-positive progenitors and derived neurons, with the subsequent shrinkage of the ganglion cell layer as a result. However, the authors had already shown in the preprint that the Atoh7-progenitors generate a deterministic branch (producing photoreceptors) and a probabilistic branch that produces retinal ganglion cells as well as horizontal and amacrine cells. If it were true that Prdmn1a knock down induces the decrease of Atoh7-positive progenitors, why would such decrease affect only the ganglion cell layer and not the inner nuclear layer, where horizontal and amacrine cells reside? The authors did not observe any shrinkage of the inner nuclear layer upon Prdmn1a knock down in the published paper.
The second, novel experiment in the published paper is a characterization of the outer nuclear layer upon knock down of both Atoh7 and Ptf1a transcription factors, as compared to wild type controls. The authors observed an increased number of densely packed photoreceptor precursors in the outer nuclear layer of double morphants as compared to control animals. Moreover, photoreceptors in double morphants had a more elongated morphology than those in controls, but the expression of the photoreceptor differentiation marker Zpr1 did not change between Atoh7- and Ptf1a- double knock down and control retinae. However, Zpr1 is a marker for only a subset of cone photoreceptors. I am therefore wondering whether testing for additional markers of photoreceptor differentiation (e.g. the pan-cone marker Gnat2 as well as markers of rod differentiation) would unravel more subtle changes of photoreceptor production in the double morphant retinae, as compared to controls. On the other hand, Gnat2 and rod differentiation markers usually come up much later in retinal development, and might not be anyway detected at the time points studied by the authors in the current paper. Hence, further experiments are necessary to answer my questions, which leads me to highlight here that scientific research is a never-ending quest for answers. Sometimes you are lucky, and you think that you have answered your original question. However, when you look closer, further questions always arise and await testing by further scientists that will open new doors, which will be crossed, further opened, or slammed shut by other scientists.
And this is both the beauty and the curse of research: small steps that open new doors that open new doors, in the endless attempt to come a little closer to…the truth of Nature.
Will we ever be able to reach such truth? We really do not know, but- in the meantime- I encourage all of you to enjoy the journey.
WHY I CHOSE THIS PREPRINT
Many studies in developmental biology have investigated the development of the vertebrate retina in zebrafish, thanks to the transparency of the larvae, which are suitable to live imaging microscopy. However, this preprint by Nerli and colleagues represents the first study that combines in vivo imaging of the developing retina with in silico simulations to explain cell fate choices of retinal progenitor cells (RPCs) that express the transcription factor Atoh7. I selected this preprint because I enjoyed the accurate characterization of fate commitment at a single cell level, a novelty in the study of retinogenesis, since previous works have dissected fate choices only at RPC population or clonal level. Moreover, there is a plot-twist in the story: Atoh7-RPCs have the potential to generate more neuronal types than previously thought… Do you want to know more? Keep reading then…
KEY FINDINGS
Atoh7-RPCs generate a photoreceptor and a second cell with variable identity across time
The authors used a transgenic fish line expressing a fluorescent reporter under the control of atoh7, one of the earliest neurogenic markers during retinogenesis. In this way, they could track cell divisions of single Atoh7-positive RPCs (Atoh7-RPCs) using live imaging of zebrafish embryos between 28 hours post-fertilization (hpf, the beginning of neurogenesis), and 60 hpf. They found that Atoh7-RPCs divided and generated two daughter cells with different fates. One daughter cell was always a precursor of photoreceptors, the light-sensing cells of the retina. Photoreceptor precursors further divided symmetrically to produce two photoreceptors. The second daughter of the Atoh7-RPCs, instead, could be a retinal ganglion cell, which is the output neuron of the retina, or a horizontal or an amacrine cell, the main inhibitory neurons in the retina. The probability that the Atoh7-RPCs would produce a retinal ganglion cell rather than an inhibitory neuron changed across time in neurogenesis. Indeed, RPCs during early (28-42 hpf) neurogenesis generated a photoreceptor precursor and most likely a retinal ganglion cell. Conversely, RPCs during late (36-60 hpf) neurogenesis generated a photoreceptor precursor and most likely an inhibitory neuron. Hence, Atoh7 RPCs differentiate along two branches: a deterministic branch that always produces photoreceptors, and a probabilistic branch that generates different cell types (a retinal ganglion cell or an inhibitory neuron) with different probabilities across time.
Faithful photoreceptor genesis is necessary for correct lamination of the retina
Next, the authors perturbed the outcome of Atoh7-RPC divisions by using morpholinos (short, antisense oligonucleotides) to knock down the expression of distinct fate determinants of the deterministic and probabilistic branches. Knocking down of Prdm1a, which is a transcription factor necessary for photoreceptor determination, impaired the deterministic branch of the Atoh7 lineage, so that photoreceptors were never produced at any time in retinogenesis. Indeed, interfering with the deterministic branch of the Atoh7-RPC division resulted in the production of a cell that initially looked like a photoreceptor precursor, but never divided further, nor differentiated to photoreceptors. Moreover, severe impairment of retina lamination occurred, with reduction of the photoreceptor layer and of the retinal ganglion cell layer. Conversely, perturbation of the probabilistic branch resulted in changed probabilities of the retinal neurons produced by Atoh7-RPC divisions, without affecting retinal lamination. Indeed, knock down of Atoh7 depleted retinal ganglion cells, but increased genesis of inhibitory neurons as well as of photoreceptors, with no significant effect on the overall thickness of the retina. Moreover, knock down of Ptf1a, a transcription factor necessary for the development of inhibitory neurons, resulted in decreased genesis of horizontal and amacrine cells, but increased production of retinal ganglion cells as well as of photoreceptors (Figure 1).
So, what do you think would happen if both Atoh7 and Ptf1a were knocked down?
Plot twist: Atoh7 RPCs have the potential to generate all retinal neurons, even bipolar cells!
Previous work1 and the current preprint show that bipolar cells, the last-born neurons in the retina, usually come from RPCs that never express Atoh7. Indeed, right before neurogenesis, a RPC divides to generate an Atoh7-positive lineage, including the probabilistic and deterministic branches, and an Atoh7-negative lineage that generates precursors of bipolar cells expressing the transcription factor Vsx1. However, the present study shows that knock down of Atoh7 and Ptf1a proteins in Atoh7-RPCs produced photoreceptor precursors as well as bipolar cells throughout the neurogenic window (Figure 1). If Atoh7-RPCs were not multipotent, they would never produce any bipolar cells upon Atoh7 and Ptf1a depletion. Hence, the authors concluded that Atoh7-RPCs potentially can generate all retinal neurons, including bipolar cells, but are eventually competent to produce only retinal ganglion, horizontal, amacrine and photoreceptor cells.
Figure 1. Interference with the probabilistic branch of Atoh7-RPCs. In control retinae, Atoh7-RPCs always generated a photoreceptor precursor (PRpr, deterministic branch) and another cell that could be either a retinal ganglion cell or an inhibitory neuron (IN) (probabilistic branch). In the morphant retina, where Atoh7 had been knocked down (Atoh7 MO), PRpr and IN production increased as compared to control during early and late neurogenesis. In Ptf1a MO retinae, RGC and PRpr genesis increased as compared to control during early and late neurogenesis. In the Atoh7+Ptf1a double MO retinae, PRpr genesis increased and BC production appeared. Figure modified from the original preprint.
A simple network of factors governs RPC fate choices
Finally, the authors developed a computational model to investigate what type of gene regulatory network involving Atoh7, Ptf1a, Prdm1a and Vsx1 would be sufficient to predict cell fate choices of Atoh7-RPCs (Figure 2). They tested two possible in silico scenarios. In the first one, it was assumed that a single transcription factor was enough to effectively inhibit the expression of a downstream factor, and, consequently, the related fate acquisition. The fate outcomes predicted by this first model, however, did not match the observed outcomes of cell divisions: indeed, the first simulation predicted the genesis of bipolar cells even in the single knock down for Atoh7 or Ptf1a, which was never detected by live imaging experiments. In other words, if it were true that, for instance, Atoh7 alone would effectively inhibit Vsx1, then we would expect bipolar cell production upon Atoh7-single knock down (Figure 2, scenario A). This simulation did not match the live imaged fate outcomes observed in the retinae of the single knock downs, nor the neuronal proportions observed in wild type embryos. In the second scenario, it was assumed that a combination of transcription factors have to exert a summed inhibition on a downstream factor to prevent the acquisition of a certain fate (Figure 2, scenario B). For instance, Atoh7 and Ptf1a were predicted to sum their inhibitions against the downstream factor Vsx1, preventing the bipolar cell fate acquisition by the Atoh7-RPCs. Indeed, it was necessary to experimentally knock down both Atoh7 and Ptf1a to observe bipolar cell genesis, while neither the Atoh7-single knock down, nor the Ptf1a-single knock down were enough to generate bipolar cells in the Atoh7-RPC, probabilistic branch (compare Figure 1 ad Figure 2, scenario B).
Figure 2. A summed inhibition is required to prevent the acquisition of a certain cell fate. In the first simulations scenario (scenario A), each transcription factor alone is able to effectively inhibit a downstream transcription factor and the acquisition of the cell fate specified by the inhibited factor. This model predicts bipolar cell (BC) production in the Atoh7-single morphant (MO) retinae, an outcome never observed with live imaging experiments (see Figure 1). In the second simulations scenario (scenario B), the effects of inhibition from more than one transcription factor are summed. This model predicts increased probability of photoreceptor (PR) and inhibitory neurons (IN) production in the Atoh7-single MO retinae, of photoreceptor and retinal ganglion cell (RGC) production in the Ptf1a-single MO retinae and of photoreceptor and bipolar cell production in the Atoh7+Ptf1a-double MO retinae. The predictions made by the simulation scenario B match the observed outcomes of live imaging experiments. Figure modified from the original preprint.
QUESTIONS FOR THE AUTHORS
1. You use morpholinos to prevent the expression of transcription factors during retinogenesis, but how specific is the action of morpholinos? Did you consider the potential off-target effects of these morpholinos? Did you think of using a conditional knock out approach to answer your questions?
2. Do you have a hypothesis about the molecular signal that starts the Atoh7 lineage? That is, did you obtain any indication about what molecular cue triggers the “firing” of Atoh7 in RPCs?
3. Did you further characterize the “aberrant” photoreceptor precursor upon Prdm1a knock down, for instance using immunohistochemistry or other approaches?
4. How do you explain the thickness reduction of the ganglion cell layer upon Prdm1a knock down?
REFERENCES
1. Vitorino, M., Jusuf, P.R., Maurus, D., Kimura, Y., Higashijima, S., and Harris, W.A. (2009). Vsx2 in the zebrafish retina: restricted lineages through derepression. Neural Develop. 4, 14. 10.1186/1749-8104-4-14.
doi: https://doi.org/10.1242/prelights.33096
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