Close

Electron Transfer from Cytochrome P450 Reductase to Cytochrome P450: Towards a Structural and Dynamic Understanding

Goutam Mukherjee, Prajwal P. Nandekar, Rebecca C. Wade

Posted on: 27 July 2020

Preprint posted on 13 June 2020

Article now published in Communications Biology at http://dx.doi.org/10.1038/s42003-020-01568-y

Passing the electron baton: researchers use an in silico strategy to investigate electron transfer from CYP450 reductase to CYP450

Selected by Zhang-He Goh

Background of preprint

Cytochrome P450s (CYP450s) are part of the superfamily of xenobiotic-metabolising enzymes that have been investigated for their effects on various drugs and toxins. One member of this family is CYP1A1, which is notable in two respects. First, CYP1A1 is an extrahepatic human drug target protein that modulates procarcinogen activation and carcinogen detoxification [1,2]. Second, the potential of CYP1A1 as a biocatalyst has been described as early as the last century [3,4]. Given these two essential roles of CYP1A1, a better understanding of CYP1A1 would be immensely useful to the pharmaceutical and bioengineering communities.

A two-electron transfer step between a redox partner and a heme cofactor (HEME) is often the rate-limiting step of the CYP catalytic cycle (preprint Figure 1) [5]. The mammalian CYPs have three domains (Fig. 1): a globular catalytic HEME-containing domain, an N-terminal transmembrane (TM) α-helical domain, and a flexible linker region connecting both the HEME-containing and the N-terminal TM domain. The redox partner of microsomal CYPs (including CYP1A1) is NADPH cytochrome P450 oxidoreductase (CPR). The CPR also contains three cofactor-binding domains (Fig. 1), which respectively bind to the flavin mononucleotide (FMN), flavin adenine dinucleotide (FAD), and nicotinamide adenine dinucleotide (NADP).

Figure 1. Mammalian CYP and CPR domains. Colours follow those in the preprint.

In their preprint, Mukherjee et al. set out to describe interactions between CYP1A1 and CPR at a detailed atomic level. They used multiresolution simulations to better understand the electron transfer process between CYP1A1 and CPR—which, in turn, informs the design of CYP1A1-targeting drugs and enhance the utility of CYP1A1 as a biocatalyst.

Key findings of preprint

Mukherjee et al. first generated a list of encounter complexes using a method known as Brownian docking (BD), an established method to predict protein-protein complexes [6]. From these, they selected six representative encounter complexes (preprint Table 1). The authors then performed structural relaxation on six encounter complexes using “soluble” molecular dynamics (MD) simulations, which are MD simulations that were conducted in aqueous solution.

After the refinement step, Mukherjee et al. narrowed their focus to just three encounter complexes simulated in the presence of a phospholipid bilayer. This led to three observations. First, the presence of CPR appeared to weaken peripheral CYP-membrane interactions. CYP1A1 became less deeply embedded in the membrane, and the CYP1A1 globular domain underwent an orientational rearrangement. Second, the FMN domain of CPR rearranged with respect to the membrane, largely caused by rearrangements of the CPR’s highly flexible linker region. Third, the FAD and NADP domains also rearranged with respect to the membrane. This made the CPR structure more compact, adopting a conformation resembling the semi-open one observed in the crystal structure.

Based on the high contact area between CYP1A1 and CPR, the authors concluded that CYP1A1 and CPR formed strongly-bound and stable transient complexes in the membrane. The CYP-FMN domain interactions involved the pairing of the positively-charged CYP1A1 proximal side with the negatively-charged FMN domain; such charge pairings have been identified as important contributors towards redox protein binding. In contrast, transient interactions formed between CYP1A1 and the NADP domain of the CPR. The authors identified two major electron transfer pathways, FMN-I548-C457-HEME and FMN-K456-C457-HEME; both corroborate other experimental data [7,8] and computational studies [9-11].

Finally, Mukherjee et al. also investigated the effects of CPR binding on the opening of ligand tunnels between the buried CYP active site and the protein surface. Specifically, the authors used conventional MD simulations, as well as random acceleration MD (RAMD) simulations of ligand egress from the CYP active site. While the authors could not draw causal inferences between CPR binding and changes in the conformations of the ligand tunnels, their RAMD simulations showed that the binding of CPR to CYP1A1 alters the distribution of egress pathways. This latter finding supports MD simulations in a recent study that CPR binding modulates ligand tunnels [12].

What I like about this preprint

In their preprint, Mukherjee et al. described their findings from a computational approach to characterising the electron transfer between CYP1A1 and CPR, its redox partner. The authors’ in silico strategy revealed key interactions between the two partners, as well as their subsequent conformational changes, that facilitate electron transfer.

The in silico approach adopted by Mukherjee et al. reinforces current experimental and computational findings on CYP450 enzymes reported by other groups. Their findings are especially pertinent when we consider key mutations that may affect the activity of these enzymes, which are implicated in both xenobiotic metabolism [7] and in certain cancers [13,14].

Future work

These insights made in CYP1A1 will enhance researchers’ understanding of the CYP450 landscape. A mechanistic investigation of the impact of CYP450 conformational structure on its kinetics may help bioengineers to optimise processes using these enzymes in synthetic biology, allowing them to synthesise complex molecules more efficiently.

The authors’ findings will also help pharmaceutical developers to design better drugs. By elucidating the effect of CYP-CPR binding on ligand binding—as well as substrate access and product release—in their preprint, Mukherjee et al. open new avenues for drug design and development. For one, anticancer drugs may be developed by targeting CYP1A1, which has been implicated in cancer aetiology. Alternatively, the headway made into CYP1A1 may help pharmacokineticists better predict drug-drug interactions involving CYP enzymes.

Like the electron cascade it described, this preprint marks the start of new inroads made in enzymatic discovery. It will be electrifying to see where it goes next.

References

[1] Androutsopoulos VP, Tsatsakis AM, Spandidos DA, Cytochrome P450 CYP1A1: wider roles in cancer progression and prevention, BMC Cancer 9(1) (2009) 187.

[2] Nandekar PP, Sangamwar AT, Cytochrome P450 1A1-mediated anticancer drug discovery: in silico findings, Expert Opin Drug Discov 7(9) (2012) 771-789.

[3] Sakaki T, Kominami S, Takemori S, Ohkawa H, Akiyoshi-Shibata M, Yabusaki Y, Kinetic studies on a genetically engineered fused enzyme between rat cytochrome P4501A1 and yeast NADPH-P450 reductase, Biochemistry 33(16) (1994) 4933-4939.

[4] Murakami H, Yabusaki Y, Sakaki T, Shibata M, Ohkawa H, A genetically engineered P450 monooxygenase: construction of the functional fused enzyme between rat cytochrome P450c and NADPH-cytochrome P450 reductase, DNA 6(3) (1987) 189-197.

[5] Guengerich FP, Mechanisms of cytochrome P450 substrate oxidation: MiniReview, J Biochem Mol Toxicol 21(4) (2007) 163-168.

[6] Motiejunas D, Gabdoulline R, Wang T, Feldman-Salit A, Johann T, Winn PJ, Wade RC, Protein-protein docking by simulating the process of association subject to biochemical constraints, Proteins 71(4) (2008) 1955-1969.

[7] Lewis BC, Mackenzie PI, Miners JO, Application of homology modeling to generate CYP1A1 mutants with enhanced activation of the cancer chemotherapeutic prodrug dacarbazine, Mol Pharmacol 80(5) (2011) 879-888.

[8] Fernández-Cancio M, Camats N, Flück CE, Zalewski A, Dick B, Frey BM, Monné R, Torán N, Audí L, Pandey AV, Mechanism of the Dual Activities of Human CYP17A1 and Binding to Anti-Prostate Cancer Drug Abiraterone Revealed by a Novel V366M Mutation Causing 17,20 Lyase Deficiency, Pharmaceuticals (Basel) 11(2) (2018).

[9] Šrejber M, Navrátilová V, Paloncýová M, Bazgier V, Berka K, Anzenbacher P, Otyepka M, Membrane-attached mammalian cytochromes P450: An overview of the membrane’s effects on structure, drug binding, and interactions with redox partners, Journal of Inorganic Biochemistry 183 (2018) 117-136.

[10] Ritacco I, Spinello A, Ippoliti E, Magistrato A, Post-Translational Regulation of CYP450s Metabolism As Revealed by All-Atoms Simulations of the Aromatase Enzyme, Journal of Chemical Information and Modeling 59(6) (2019) 2930-2940.

[11] Fishelovitch D, Hazan C, Hirao H, Wolfson HJ, Nussinov R, Shaik S, QM/MM study of the active species of the human cytochrome P450 3A4, and the influence thereof of the multiple substrate binding, J Phys Chem B 111(49) (2007) 13822-13832.

[12] Ritacco I, Saltalamacchia A, Spinello A, Ippoliti E, Magistrato A, All-Atom Simulations Disclose How Cytochrome Reductase Reshapes the Substrate Access/Egress Routes of Its Partner CYP450s, The Journal of Physical Chemistry Letters 11(4) (2020) 1189-1193.

[13] Freedland J, Cera C, Fasullo M, CYP1A1 I462V polymorphism is associated with reduced genotoxicity in yeast despite positive association with increased cancer risk, Mutat Res 815 (2017) 35-43.

[14] Ezzeldin N, El-Lebedy D, Darwish A, El-Bastawisy A, Hassan M, Abd El-Aziz S, Abdel-Hamid M, Saad-Hussein A, Genetic polymorphisms of human cytochrome P450 CYP1A1 in an Egyptian population and tobacco-induced lung cancer, Genes Environ 39 (2017) 7-7.

Acknowledgements

The preLight author is grateful to the preprint authors for their suggestions regarding Figure 1.

Tags: cyp1a1, cytochrome p450, metabolism, redox

doi: https://doi.org/10.1242/prelights.22995

Read preprint (No Ratings Yet)

Have your say

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

Sign up to customise the site to your preferences and to receive alerts

Register here

Also in the bioinformatics category:

Functional Diversity of Memory CD8 T Cells is Spatiotemporally Imprinted

Miguel Reina-Campos, Alexander Monell, Amir Ferry, et al.

Selected by 22 August 2024

Marina Schernthanner

Bioinformatics

Enhancer-driven cell type comparison reveals similarities between the mammalian and bird pallium

Nikolai Hecker , Niklas Kempynck , David Mauduit, et al.

Selected by 02 July 2024

Rodrigo Senovilla-Ganzo

Bioinformatics

Expressive modeling and fast simulation for dynamic compartments

Till Köster, Philipp Henning, Tom Warnke, et al.

Selected by 18 April 2024

Benjamin Dominik Maier

Systems Biology

Also in the biophysics category:

Global coordination of protrusive forces in migrating immune cells

Patricia Reis-Rodrigues, Nikola Canigova, Mario J. Avellaneda, et al.

Selected by 10 October 2024

yohalie kalukula

Biophysics

Engineered Nanotopographies Induce Transient Openings in the Nuclear Membrane

Einollah Sarikhani, Vrund Patel, Zhi Li, et al.

Selected by 23 September 2024

Sristilekha Nath

Bioengineering

Spiral-eyes: A soft active matter model of in vivo corneal epithelial cell migration

Kaja Kostanjevec, Rastko Sknepnek, Jon Martin Collinson, et al.

Selected by 03 September 2024

Prasanna Padmanaban

Biophysics

Also in the pharmacology and toxicology category:

G6b-B antibody-based cis-acting platelet receptor inhibitors (CAPRIs) as a new family of anti-thrombotic therapeutics

Alexandra Mazharian, Ophélie Bertin, Amrita Sarkar, et al.

Selected by 03 June 2024

Simon Cleary

Pharmacology and Toxicology

Pervasive sublethal effects of agrochemicals as contributing factors to insect decline

Lautaro Gandara, Richard Jacoby, François Laurent, et al.

Selected by 07 February 2024

Roberto Amadio

Animal Behavior and Cognition

Mixed Alkyl/Aryl Phosphonates Identify Metabolic Serine Hydrolases as Antimalarial Targets

John M. Bennett, Sunil K. Narwal, Stephanie Kabeche, et al.

Selected by 02 February 2024

Zhang-He Goh

Microbiology

preLists in the bioinformatics category:

‘In preprints’ from Development 2022-2023

A list of the preprints featured in Development's 'In preprints' articles between 2022-2023

 



List by Alex Eve, Katherine Brown

9th International Symposium on the Biology of Vertebrate Sex Determination

This preList contains preprints discussed during the 9th International Symposium on the Biology of Vertebrate Sex Determination. This conference was held in Kona, Hawaii from April 17th to 21st 2023.

 



List by Martin Estermann

Alumni picks – preLights 5th Birthday

This preList contains preprints that were picked and highlighted by preLights Alumni - an initiative that was set up to mark preLights 5th birthday. More entries will follow throughout February and March 2023.

 



List by Sergio Menchero et al.

Fibroblasts

The advances in fibroblast biology preList explores the recent discoveries and preprints of the fibroblast world. Get ready to immerse yourself with this list created for fibroblasts aficionados and lovers, and beyond. Here, my goal is to include preprints of fibroblast biology, heterogeneity, fate, extracellular matrix, behavior, topography, single-cell atlases, spatial transcriptomics, and their matrix!

 



List by Osvaldo Contreras

Single Cell Biology 2020

A list of preprints mentioned at the Wellcome Genome Campus Single Cell Biology 2020 meeting.

 



List by Alex Eve

Antimicrobials: Discovery, clinical use, and development of resistance

Preprints that describe the discovery of new antimicrobials and any improvements made regarding their clinical use. Includes preprints that detail the factors affecting antimicrobial selection and the development of antimicrobial resistance.

 



List by Zhang-He Goh

Also in the pharmacology and toxicology category:

FENS 2020

A collection of preprints presented during the virtual meeting of the Federation of European Neuroscience Societies (FENS) in 2020

 



List by Ana Dorrego-Rivas

COVID-19 / SARS-CoV-2 preprints

List of important preprints dealing with the ongoing coronavirus outbreak. See http://covidpreprints.com for additional resources and timeline, and https://connect.biorxiv.org/relate/content/181 for full list of bioRxiv and medRxiv preprints on this topic

 



List by Dey Lab, Zhang-He Goh

1

Drug use in special populations

Any drugs that are being used in special populations: Patients with liver and kidney failure, in paediatrics, in geriatrics, and in pregnant or lactating patients. Includes the discovery of factors that could potentially affect drug use in these special populations.

 



List by Zhang-He Goh

Toxicology of toxicants, existing therapeutics, and investigational drugs

Preprints that describe the toxicology of environmental pollutants and existing and upcoming drugs. Includes both toxicokinetics and toxicodynamics, as well as technological improvements that will help in the characterisation of this field.

 



List by Zhang-He Goh

Antimicrobials: Discovery, clinical use, and development of resistance

Preprints that describe the discovery of new antimicrobials and any improvements made regarding their clinical use. Includes preprints that detail the factors affecting antimicrobial selection and the development of antimicrobial resistance.

 



List by Zhang-He Goh

Anticancer agents: Discovery and clinical use

Preprints that describe the discovery of anticancer agents and their clinical use. Includes both small molecules and macromolecules like biologics.

 



List by Zhang-He Goh

Advances in Drug Delivery

Advances in formulation technology or targeted delivery methods that describe or develop the distribution of small molecules or large macromolecules to specific parts of the body.

 



List by Zhang-He Goh
Close