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The Trypanosoma brucei subpellicular microtubule array is organized into functionally discrete subdomains defined by microtubule associated proteins

Amy N. Sinclair, Christine T. Huynh, Thomas E. Sladewski, Jenna L. Zuromski, Amanda E. Ruiz, Christopher L. de Graffenried

Preprint posted on November 09, 2020 https://www.biorxiv.org/content/10.1101/2020.11.09.375725v1

Looking into T. brucei microtubule arrays.

Selected by Mariana De Niz

Categories: cell biology

Background

A key contributor to T. brucei pathogenesis is the highly asymmetric shape of its body, as it aids the parasite’s survival within the insect and mammalian vector. The trypomastigote shape of T. brucei is produced by a single layer of microtubules that underlies the plasma membrane, called the subpellicular array. A flagellum is attached to the cell surface by the flagellum attachment zone, which is comprised of a filament that is inserted between the subpellicular microtubules. Microtubules are inherently dynamic cytoskeletal polymers, and their length and activity can be altered to perform essential functions, including providing tracks for intracellular trafficking and forming the mitotic spindle. They can also be bundled to create stable structures that collectively propagate force, such as in the flagellar axoneme, which provides motility.

In T. brucei, as the flagellum beats, it deforms the subpellicular microtubule array and creates “cellular waveforms” that define the distinctive corkscrew-like motility pattern of T. brucei. The ability to regulate microtubule dynamics and flexibility within different domains of the array would allow the parasite to optimize the transmission of energy generated by the flagellar beat, and channel it into productive motility. The subpellicular array microtubules appear to be highly stable, and remain intact throughout the cell cycle, but little is known about the pathways that tune microtubule properties in trypanosomatids. In their work, Sinclair et al (1) have identified a set of T. brucei proteins that localize to different subdomains of the subpellicular array. Moreover, they characterize the location and function of the array-associated protein PAVE1, which is exclusive to kinetoplastids, and is a component of the inter-microtubule crosslinking fibrils present within the posterior subdomain. Altogether, the authors show that the subpellicular array contains various differentially localized array-associated proteins that likely function to locally tune the biophysical characteristics of the microtubules.

Figure 1. Schematics of the cytoskeleton and cell division in Trypanosoma brucei. (From Ref 1).

Key findings and developments

PAVE1 localizes to the inter-microtubule crosslinks of the subpellicular array at the cell posterior. PAVE1 had been previously observed to localize at the posterior and ventral edge of the subpellicular array. In this work, the authors went on to determine if PAVE1 co-localizes to regions of new microtubule growth throughout their cell cycle, by comparing PAVE1 distribution with antibody labelling of YL1/2, which recognizes the terminal tyrosine residue of alpha tubulin. Terminal tyrosination is a hallmark of newly polymerized tubulin, and a marker of array growth during the cell cycle. The authors describe PAVE1 and YL1/2 localization with respect to the number and morphology of nuclei and kinetoplasts (as markers of cell cycle). The localization pattern suggests that PAVE1 is stably associated with the array microtubules at the posterior end of the cell throughout the cell cycle, and is recruited to the nascent posterior during its formation. The authors went on to generate subpellicular array sheets from immunogold-labelled cytoskeletons, and compared PAVE1 distribution with the localization pattern of TAT1, an antibody that recognizes T. brucei a-tubulin. Comparison of TAT1 and PAVE1 showed that PAVE1 preferentially localizes to the outside ventral walls of array microtubules at the cell posterior, and to the inter-microtubule crosslinks that remained after sheet preparation. This pattern suggests that PAVE1 may be part of the inter-microtubule crosslinking fibrils present in the posterior subdomain of the array.

PAVE1 maintains microtubule length at the posterior subpellicular array. The authors went on to determine how PAVE1 and the inter-microtubule crosslinks are incorporated in the array. For this, they used HaloTag technology to visualize the localization of newly synthesized PAVE1, and how its distribution changed over time throughout 8 hours.

The authors then performed PAVE1 RNAi to test if PAVE1 is required to maintain the microtubules of the posterior array, or if array truncation in PAVE1-depleted cells is the result of aberrant cell division. They first developed a strategy to identify cells that had completed cell division prior to PAVE1 RNAi induction, and restricted the analysis to 1N2K cells to select for those that had progressed well into G1 phase at the time of PAVE1 depletion. Using an anti-tubulin antibody, the authors observed that the posterior array of PAVE1 RNAi cells at 6h and 4h was significantly shorter than at 0h. However, the length of the array between the nucleus and posterior kinetoplast was the same at all 3 time points. This suggests that PAVE1 is required to maintain the extended tapering portion of the microtubules of the cell posterior independent of its potential function during formation of a nascent cell posterior during cell division.

PAVE2 and TbAIR9 are two potential interacting partners of PAVE1. The authors investigated whether PAVE1 was part of a complex of proteins that form the inter-microtubule crosslinks at the cell posterior. To do so, they endogenously tagged both PAVE1 alleles with mNeonGreen (mNG) at the N-terminus, and immunoprecipitated mNG-PAVE1 using a mNeonTrap antibody. Two potential interacting partners were identified, namely Tb927.9.11540 a previously uncharacterized protein of 51 kDa (henceforth named PAVE2), and TbAIR9 (Tb927.11.17000, of 110 kDa). PAVE2 was located at the posterior and ventral edge of the cell which matched the localisation pattern of PAVE1, while TbAIR9 was associated with the entire array.

PAVE1 and PAVE2 require each other for stability and localization. PAVE2 was found to be conserved in kinetoplastids. PAVE2 RNAi cells were unable to divide after 4 days of RNAi induction, and showed similar division defects and posterior truncation phenotypes as PAVE1 RNAi cells. Having confirmed mirrored phenotypes upon depletion of either PAVE1 or PAVE2, the authors went on to explore what happened to localization upon depletion of either. They found that as PAVE1 levels decreased at the posterior subpellicular array, so did PAVE2, and vice versa, suggesting that PAVE1 and PAVE2 require each other for localization and protein stability inside the cell.

PAVE1 and PAVE2 form a microtubule-associated complex in vitro. As the results obtained thus far suggested that PAVE1 and PAVE2 might be part of a complex, the authors went on to test this hypothesis in vitro, and indeed suggest that PAVE1 and PAVE2 form a hetero-oligomer responsible for the construction and maintenance of the tapered cell posterior. They found also that although the PAVE complex localizes to the inter-microtubule crosslinks of the T. brucei posterior subpellicular array in vivo, they do not appear to have the capacity to crosslink microtubules in vitro. Another important observation was that the PAVE complex bound to microtubules in discrete, static, patches, which suggests that complex may recognize specific regions of microtubules. Moreover, the authors also suggest that the PAVE complex binds directly to the microtubule lattice, and may recognize a specific lattice structure, rather than a post-translational modification in the tubulin tails.

TbAIR9 controls the distribution of PAVE1 in the subpellicular array and is a global regulator of subpellicular array-associated protein localization.. Aside of PAVE2, the other potential interacting partner of PAVE1 is TbAIR9. The authors began by testing the effect of depleting TbAIR9 by RNAi on PAVE1 localisation, and found that in TbAIR9 RNAi cells, PAVE1 labelling was less intense at the cell posterior and more intense in the cell anterior compared to controls. However, PAVE1 protein levels were unaltered, indicating that TbAIR9 depletion only affects the ability of PAVE1 to localize to the posterior subdomain. Doing the mirror experiments, the authors showed that depleting PAVE1 did not affect TbAIR9 stability or localization, except for the absence at the posterior subpellicular array in cells where the posterior was truncated. With this and further investigation, the authors conclude that TbAIR9 is not necessary to build the inter-microtubule crosslinks present in the array, but it is required to properly limit the distribution of PAVE1 to the cell posterior.

The results observed upon depletion of TbAIR9 in terms of PAVE1 redistribution suggested that TbAIR9 may regulate the localization of other cytoskeletal proteins in the subpellicular array. To test this, the authors used two proteins localized at different domains of the array: Tb927.9.10790 (10790) (annotated in TrypTag as localizing to the middle of the subpellicular array), and Tb927.11.1840 (1840) (annotated in TrypTag as localized to the anterior array). Immunofluorescence imaging of both proteins on extracted cytoskeletons confirmed the expected localization and showed their stable association with the cytoskeleton. In TbAIR9 RNAi cells, both proteins lost this restricted localization, and were instead found either weakly throughout the array, or at the posterior and anterior arrays, respectively. Moreover, the protein levels of protein 10790 seemed to decrease, while levels of 1840 increased. This suggests that TbAIR9 plays a key role in organizing and confining array-associated proteins to distinct domains within the array.

What I like about this preprint

I found the work very interesting, both in terms of cell biology, and in that it advances our understanding of Trypanosoma brucei. Moreover I found the range of methods used and the range of questions addressed very varied and complete.

References

  1. Sinclair et al, The T. brucei subpellicular microtubule array is organized into functionally discrete subdomains defined by microtubule associated proteins, bioRxiv, 2020.

 

Posted on: 22nd December 2020 , updated on: 2nd January 2021

doi: https://doi.org/10.1242/prelights.26610

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Author's response

Amy Sinclair, Chris de Graffenried shared

Open questions

1.Is there any suggestion of other interacting partners with PAVE1- perhaps transient ones?

We are currently using proximity-based methods to attempt to identify additional PAVE1/2 interactors. It is very likely that there are additional interacting partners, but they may require intact microtubules to associate with PAVE1/2. The subpellicular microtubules were disrupted and depolymerized for our current immunoprecipitation experiments.

2.You mention throughout your work the relevance of microtubules, including their role in providing tracks for intracellular trafficking. Do you know how depletion of any of the members of the PAVE complex impacts on intracellular trafficking?

One of the fascinating things about Trypanosoma brucei is that it appears to lack the dynamic cytosolic microtubules that are present in many other eukaryotes, making it likely that vesicular traffic in T. brucei does not rely on microtubules as tracks. Because of this, we feel that it is unlikely that PAVE1/2 plays a role in intracellular trafficking. The related trypanosomatids Leishmania spp and Trypanosoma cruzi do have specialized sets of cytosolic microtubules that are associated with certain portions of the secretory pathway, but the precise function of these microtubules is unknown.

3.You also mention the role of microtubules in creating stable structures that propagate force, aiding for instance, flagellar beating and parasite motility. In vitro, what do you expect in terms of motility upon depletion of any of the components of the PAVE complex?

In the case of PAVE1, the loss of the tapered posterior is likely to change the flow of the media surrounding the cell during motility, which we expect would cause changes in motility. We are actively looking for factors that are not necessary for the construction of the inter-microtubule crosslinks but may tune their properties and those of the microtubules themselves. The absence of these factors may cause a local loss of flexibility in certain portions of the array, which may cause ruptures in the microtubules or increased gaps between them, both of which would be very detrimental to the cell and its motility.

4.What would you expect any of the mutant parasites (for any of the components of the PAVE complex) to behave within a host?

Since loss of PAVE1, PAVE2, and TbAIR9 all lead to very severe cell division effects in culture, we would expect that parasites depleted of these proteins would not be able to infect their usual hosts. If there are defects in motility in the bloodstream form of the parasite, they would not be able to clear surface-bound antibody from the cells surface, which would likely lead to rapid detection and lysis by the host immune system. The loss of the tapered trypomastigote cell architecture in the case of PAVE1/2 depletion hampers cell division, which would impair cell viability in the insect and mammalian hosts. This is also true for the array master regulator, TbAIR9. TbAIR9 depletion scrambles the composition and identity of the differentially localized subdomains throughout the array, which also inhibits the ability of cells to properly divide. So it is clear that the specialized architecture of the array that is maintained by these subdomains is required for cell viability, and would lead to survival defects in the insect and mammalian hosts.

5.You mention in your conclusion that the arrangement of proteins within the array is likely to tune the local properties of the array microtubules and create the asymmetric shape of the cell. Can you expand on this idea, following the interesting findings you present in this work?

There is much to be discovered about the purpose of these subdomains. The three subdomains we identified each occur at unique places with respect to the flagellum in T. brucei. The posterior subdomain (to which PAVE1/2 localizes) comprises an area without flagellar attachment, as the flagellum exits onto the cell surface ~4-5 µm from the posterior edge of the array. The middle subdomain (protein 10790) covers the widest point of the cell body, directly after the flagellum exits the flagellar pocket and is attached to the cell surface. This includes the area where the flagellar pocket, kinetoplast, and nucleus are located. The flagellum is attached throughout the entirety of the anterior subdomain (protein 1840), where the array rapidly tapers to a narrow point. The flagellar beat in T. brucei can initiate from either its base or tip and creates asymmetric waveforms along the cell body. It is likely that each of these three subdomain regions experience different amounts of force during flagellar beating. The unique combination of array-associated proteins at each subdomain may be present to regulate the flexibility, stabilize, or repair microtubules in response to these differential forces. In addition to force response, subdomain proteins may also be present to create the curvature and helicity of array microtubules and retain cell shape throughout the cell cycle, like PAVE1 and PAVE2. We are actively undertaking functional studies on these newly identified subdomain proteins to elucidate their role in their respective subdomains, which will inform on their structural and regulatory purposes.

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