Close

Rab11b-mediated integrin recycling promotes brain metastatic adaptation and outgrowth

Erin N. Howe, Miranda D. Burnette, Melanie E. Justice, James W. Clancy, Ian H. Guldner, Patricia M. Schnepp, Victoria Hendrick, Uma K. Aryal, Alicia T. Specht, Jun Li, Crislyn D’Souza-Schorey, Jeremiah Z. Zartman, Siyuan Zhang

Preprint posted on 11 June 2019 https://www.biorxiv.org/content/10.1101/666750v1

Article now published in Nature Communications at http://dx.doi.org/10.1038/s41467-020-16832-2

How do breast cancer cells adapt to the brain microenvironment during metastasis? This recent preprint provides new insights into the role of Rab11b-mediated endosomal recycling.

Selected by Beverley Wilson

Background:

Incidence of breast cancer brain metastases is increasing (Kotecki et al. 2018) and is seen as an urgent clinical problem. Brain metastases are hard to treat using standard chemotherapeutic agents, as many of these are unable to cross the blood-brain barrier, leading to an urgent need for therapeutic advance.

Disseminated tumour cells from the original primary breast tumour are known to persist in the brain for long periods before metastatic disease becomes apparent (Harper et al. 2016; Hosseini et al. 2016). Adaptation to the brain by these disseminated cells requires their engagement with the brain microenvironment, which differs vastly from that of the primary tumour.

The interaction of tumour cells with the brain microenvironment is mainly mediated by proteins on the cell surface, including a range of cell matrix adhesion receptors such as integrins. It is increasingly being shown that control of the cell surface proteome through vesicular trafficking can play a key role in regulating cancer progression, including cancer cell migration and metastasis (Caswell et al. 2008; Ye et al. 2016). Members of the RabGTPase family are key regulators of vesicular trafficking and therefore of the cell surface proteome.

The role of endosomal trafficking in regulating the cell surface proteome of disseminated breast cancer cells in the brain metastatic environment and the impact of this on their adaptation to this environment is poorly understood. Howe et al. investigate this in detail and draw conclusions which could impact the clinical approaches to treating breast cancer brain metastases.

Key findings:

Initially, the authors observed temporal transcriptional changes during breast cancer brain metastases development by RNA sequencing of both early colonising cells in the brain and late-stage brain metastases. They found extensive transcriptional reprogramming upon metastatic adaptation, with 108 genes significantly upregulated in overt brain metastases. In order to identify which of these were able to functionally drive brain metastasis the authors used a Drosophila melanogaster tumour model, in which tumours develop in the eye disc and progressively invade the adjacent brain tissue. This led to the identification of a variety of genes which promote tumour growth and spread.

One of these, Rab11b, was also shown to be increased 15-fold in brain metastases when compared to matched breast cancer cell lines, while no change in expression was observed for the other Rab11-family members Rab11a or Rab25 (Rab11c). This suggests that the brain microenvironment specifically influences Rab11b expression, indicating that Rab11b could facilitate brain metastasis.

The authors demonstrated that Rab11b plays a causal role in breast cancer brain metastasis as depletion of Rab11b is sufficient to decrease brain metastasis in a mouse model. From this the authors hypothesised that the upregulation of Rab11b in disseminated cancer cells during early adaptation to the brain microenvironment could provide a mechanism for disseminated tumour cells to regulate their cell surface proteome allowing survival.

To identify the subset of surface proteins which are dependent on Rab11b for recycling, the authors carried out mass spectrometry analysis of the cell surface proteome of Rab11b-depleted cells. Rab11b-depleted cells showed a dramatic reduction in the level of adhesion proteins present on their surface. One of these, integrin β1, was shown to be strongly expressed in brain metastases and the authors showed that Rab11b-mediated control of integrin β1 cell surface levels enables successful interaction with the brain extracellular matrix and facilitates tumour cell survival.

Finally, the authors sought a therapeutic strategy to target Rab11b. They did this by using the statins, pitavastatin and simvastatin, which inhibit the mevalonate pathway, thereby preventing the geranylgeranylation of RabGTPases leaving them unable to be localised to membranes and therefore non-functional. The use of these statins inhibited the proliferation of breast cancer cells on decellularized brain matrix and Rab11b-mediated recycling of integrin β1. The statins also dramatically reduced brain metastasis incidence in in vivo mouse models, and led to an increase in survival.

Together these experiments showed the importance of endosomal recycling by a less well studied RabGTPase, Rab11b, in the adaptation of breast cancer cells to the challenging brain metastatic microenvironment, allowing eventual proliferation (Figure 1). The authors also provide a strong rationale for the repurposing of statins to prevent breast cancer brain metastasis, indicating that this could be a clinically practical strategy.

Figure 1: Abstract figure from the preprint. Rab11b up-regulation in the brain microenvironment promotes the recycling of cargo proteins required for breast cancer brain metastasis. Inhibition of the mevalonate pathway with statins prevents geranylgeranylation of Rab11b, decreasing cargo recycling, and inhibiting brain metastasis.

Why I think this work is important:

Regulation of the cell surface proteome represents an important mechanism by which cancer progression is driven. It is known that the dependence on different RabGTPase recycling pathways varies greatly in different environments, including between 2D and 3D environments. Therefore, I like how the authors of this study made use of a variety of applicable models, from soft agar, to decellularized brain matrix and in vivo mouse models.

I’m particularly impressed that this work contains the development of the authors’ hypothesis from the initial identification of the mechanism all the way through to potential therapeutic strategies. The in vivo work using statins to reduce brain metastatic incidence shows the potential for using these in the chemoprevention of breast cancer brain metastasis, giving opportunity for driving much needed therapeutic advance.

Questions for the authors:

  1. As other cancer types which metastasise to the brain will be adapting to the same niche, is it likely that this mechanism of adaption of breast cancer cells to the brain microenvironment could be common to other cancer types?
  2. Rab11b is known to be expressed in non-epithelial tissues, including brain tissue. Could there be a link between this and why breast cancer cells specifically upregulate Rab11b and not other Rab11 family subtypes?

Bibliography:

Caswell, P.T., Chan, M., Lindsay, A.J., McCaffrey, M.W., Boettiger, D., and Norman, J.C. (2008). Rab- coupling protein coordinates recycling of α5β1 integrin and EGFR1 to promote cell migration in 3D microenvironments. J. Cell Biol. 183(1):143-155.

Harper, K.L., Sosa, M.S., Entenberg, D., Hosseini, H., Cheung, J.F., Nobre, R., et al. (2016). Mechanism of early dissemination and metastasis in Her2+ mammary cancer. Nature 540(7634):588-592

Hosseini, H., Obradović, M.M.S., Hoffmann, M., Harper, K.L., Sosa, M.S., Werner-Klein, M., et al. (2016). Early dissemination seeds metastasis in breast cancer. Nature 540(7634):552-558

Kotecki, N., Lefranc, F., Devriendt, D., and Awada, A. (2018). Therapy of breast cancer brain metastas es: challenges, emerging treatments and perspectives. Ther Adv Med Oncol. 10:1758835918780312

Ye, Q.­H., Zhu, W.­W., Zhang, J.­B., Qin, Y., Lu, M., Lin, G.­L., et al. (2016). GOLM1 modulates EGFR/RTK cell­surface recycling to drive hepatocellular carcinoma metastasis. Cancer Cell 30(3):444-458

Tags: breast cancer, endosomal recycling, metastasis

Posted on: 19 July 2019

doi: https://doi.org/10.1242/prelights.12279

Read preprint (No Ratings Yet)

Author's response

Siyuan Zhang shared

Thank you again for your fantastic summary of our work and excellent questions!

Broadly, yes, we propose that the general mechanism of recycling or trafficking is likely used by all cancer cells as they adapt to all metastatic sites, such as the brain. However, the cell surface proteome could vary dramatically among different primary cancer types. While recycling is one of the understudied core mechanisms for metastatic adaptation in our view, we do think the interactions will be highly cell type and context-dependent, which will require substantially more investigation. As with everything in biology, context is key.

Equally intriguing is your second question. Our data suggested that Rab11b is the isoform up-regulated in the brain based on a requirement for Rab11b specifically in the brain. How can we explain the isoform specificity? There are two options that we have considered. 1 – there is a transcriptional program activated in breast cancer cells upon arrival in the brain, which directs Rab11b up-regulation. 2 – there is a specific requirement (at the brain environment) for Rab11b based on the necessity of Rab11b-mediated recycling. There are a number of studies, including one from our lab, showing that there is transcriptional reprogramming in brain metastatic breast cancer cells. However, there is a relative lack of information about both Rab11b transcriptional regulation and the mechanisms governing Rab11b-mediated recycling, which makes it difficult at this stage to determine how or why breast cancer cells up-regulate Rab11b upon arrival in the brain.

Have your say

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

Sign up to customise the site to your preferences and to receive alerts

Register here

preLists in the biochemistry category:

Preprint Peer Review – Biochemistry Course at UFRJ, Brazil

Communication of scientific knowledge has changed dramatically in recent decades and the public perception of scientific discoveries depends on the peer review process of articles published in scientific journals. Preprints are key vehicles for the dissemination of scientific discoveries, but they are still not properly recognized by the scientific community since peer review is very limited. On the other hand, peer review is very heterogeneous and a fundamental aspect to improve it is to train young scientists on how to think critically and how to evaluate scientific knowledge in a professional way. Thus, this course aims to: i) train students on how to perform peer review of scientific manuscripts in a professional manner; ii) develop students' critical thinking; iii) contribute to the appreciation of preprints as important vehicles for the dissemination of scientific knowledge without restrictions; iv) contribute to the development of students' curricula, as their opinions will be published and indexed on the preLights platform. The evaluations will be based on qualitative analyses of the oral presentations of preprints in the field of biochemistry deposited in the bioRxiv server, of the critical reports written by the students, as well as of the participation of the students during the preprints discussions.

 



List by Marcus Oliveira

CellBio 2022 – An ASCB/EMBO Meeting

This preLists features preprints that were discussed and presented during the CellBio 2022 meeting in Washington, DC in December 2022.

 



List by Nadja Hümpfer et al.

20th “Genetics Workshops in Hungary”, Szeged (25th, September)

In this annual conference, Hungarian geneticists, biochemists and biotechnologists presented their works. Link: http://group.szbk.u-szeged.hu/minikonf/archive/prg2021.pdf

 



List by Nándor Lipták

Fibroblasts

The advances in fibroblast biology preList explores the recent discoveries and preprints of the fibroblast world. Get ready to immerse yourself with this list created for fibroblasts aficionados and lovers, and beyond. Here, my goal is to include preprints of fibroblast biology, heterogeneity, fate, extracellular matrix, behavior, topography, single-cell atlases, spatial transcriptomics, and their matrix!

 



List by Osvaldo Contreras

ASCB EMBO Annual Meeting 2019

A collection of preprints presented at the 2019 ASCB EMBO Meeting in Washington, DC (December 7-11)

 



List by Madhuja Samaddar et al.

EMBL Seeing is Believing – Imaging the Molecular Processes of Life

Preprints discussed at the 2019 edition of Seeing is Believing, at EMBL Heidelberg from the 9th-12th October 2019

 



List by Dey Lab

Cellular metabolism

A curated list of preprints related to cellular metabolism at Biorxiv by Pablo Ranea Robles from the Prelights community. Special interest on lipid metabolism, peroxisomes and mitochondria.

 



List by Pablo Ranea Robles

MitoList

This list of preprints is focused on work expanding our knowledge on mitochondria in any organism, tissue or cell type, from the normal biology to the pathology.

 



List by Sandra Franco Iborra

Also in the cancer biology category:

CSHL 87th Symposium: Stem Cells

Preprints mentioned by speakers at the #CSHLsymp23

 



List by Alex Eve

Journal of Cell Science meeting ‘Imaging Cell Dynamics’

This preList highlights the preprints discussed at the JCS meeting 'Imaging Cell Dynamics'. The meeting was held from 14 - 17 May 2023 in Lisbon, Portugal and was organised by Erika Holzbaur, Jennifer Lippincott-Schwartz, Rob Parton and Michael Way.

 



List by Helen Zenner

CellBio 2022 – An ASCB/EMBO Meeting

This preLists features preprints that were discussed and presented during the CellBio 2022 meeting in Washington, DC in December 2022.

 



List by Nadja Hümpfer et al.

Fibroblasts

The advances in fibroblast biology preList explores the recent discoveries and preprints of the fibroblast world. Get ready to immerse yourself with this list created for fibroblasts aficionados and lovers, and beyond. Here, my goal is to include preprints of fibroblast biology, heterogeneity, fate, extracellular matrix, behavior, topography, single-cell atlases, spatial transcriptomics, and their matrix!

 



List by Osvaldo Contreras

Single Cell Biology 2020

A list of preprints mentioned at the Wellcome Genome Campus Single Cell Biology 2020 meeting.

 



List by Alex Eve

ASCB EMBO Annual Meeting 2019

A collection of preprints presented at the 2019 ASCB EMBO Meeting in Washington, DC (December 7-11)

 



List by Madhuja Samaddar et al.

Lung Disease and Regeneration

This preprint list compiles highlights from the field of lung biology.

 



List by Rob Hynds

Anticancer agents: Discovery and clinical use

Preprints that describe the discovery of anticancer agents and their clinical use. Includes both small molecules and macromolecules like biologics.

 



List by Zhang-He Goh

Biophysical Society Annual Meeting 2019

Few of the preprints that were discussed in the recent BPS annual meeting at Baltimore, USA

 



List by Joseph Jose Thottacherry

Also in the cell biology category:

‘In preprints’ from Development 2022-2023

A list of the preprints featured in Development's 'In preprints' articles between 2022-2023

 



List by Alex Eve, Katherine Brown

preLights peer support – preprints of interest

This is a preprint repository to organise the preprints and preLights covered through the 'preLights peer support' initiative.

 



List by preLights peer support

The Society for Developmental Biology 82nd Annual Meeting

This preList is made up of the preprints discussed during the Society for Developmental Biology 82nd Annual Meeting that took place in Chicago in July 2023.

 



List by Joyce Yu, Katherine Brown

CSHL 87th Symposium: Stem Cells

Preprints mentioned by speakers at the #CSHLsymp23

 



List by Alex Eve

Journal of Cell Science meeting ‘Imaging Cell Dynamics’

This preList highlights the preprints discussed at the JCS meeting 'Imaging Cell Dynamics'. The meeting was held from 14 - 17 May 2023 in Lisbon, Portugal and was organised by Erika Holzbaur, Jennifer Lippincott-Schwartz, Rob Parton and Michael Way.

 



List by Helen Zenner

9th International Symposium on the Biology of Vertebrate Sex Determination

This preList contains preprints discussed during the 9th International Symposium on the Biology of Vertebrate Sex Determination. This conference was held in Kona, Hawaii from April 17th to 21st 2023.

 



List by Martin Estermann

Alumni picks – preLights 5th Birthday

This preList contains preprints that were picked and highlighted by preLights Alumni - an initiative that was set up to mark preLights 5th birthday. More entries will follow throughout February and March 2023.

 



List by Sergio Menchero et al.

CellBio 2022 – An ASCB/EMBO Meeting

This preLists features preprints that were discussed and presented during the CellBio 2022 meeting in Washington, DC in December 2022.

 



List by Nadja Hümpfer et al.

Fibroblasts

The advances in fibroblast biology preList explores the recent discoveries and preprints of the fibroblast world. Get ready to immerse yourself with this list created for fibroblasts aficionados and lovers, and beyond. Here, my goal is to include preprints of fibroblast biology, heterogeneity, fate, extracellular matrix, behavior, topography, single-cell atlases, spatial transcriptomics, and their matrix!

 



List by Osvaldo Contreras

EMBL Synthetic Morphogenesis: From Gene Circuits to Tissue Architecture (2021)

A list of preprints mentioned at the #EESmorphoG virtual meeting in 2021.

 



List by Alex Eve

FENS 2020

A collection of preprints presented during the virtual meeting of the Federation of European Neuroscience Societies (FENS) in 2020

 



List by Ana Dorrego-Rivas

Planar Cell Polarity – PCP

This preList contains preprints about the latest findings on Planar Cell Polarity (PCP) in various model organisms at the molecular, cellular and tissue levels.

 



List by Ana Dorrego-Rivas

BioMalPar XVI: Biology and Pathology of the Malaria Parasite

[under construction] Preprints presented at the (fully virtual) EMBL BioMalPar XVI, 17-18 May 2020 #emblmalaria

 



List by Dey Lab, Samantha Seah

1

Cell Polarity

Recent research from the field of cell polarity is summarized in this list of preprints. It comprises of studies focusing on various forms of cell polarity ranging from epithelial polarity, planar cell polarity to front-to-rear polarity.

 



List by Yamini Ravichandran

TAGC 2020

Preprints recently presented at the virtual Allied Genetics Conference, April 22-26, 2020. #TAGC20

 



List by Maiko Kitaoka et al.

3D Gastruloids

A curated list of preprints related to Gastruloids (in vitro models of early development obtained by 3D aggregation of embryonic cells). Updated until July 2021.

 



List by Paul Gerald L. Sanchez and Stefano Vianello

ECFG15 – Fungal biology

Preprints presented at 15th European Conference on Fungal Genetics 17-20 February 2020 Rome

 



List by Hiral Shah

ASCB EMBO Annual Meeting 2019

A collection of preprints presented at the 2019 ASCB EMBO Meeting in Washington, DC (December 7-11)

 



List by Madhuja Samaddar et al.

EMBL Seeing is Believing – Imaging the Molecular Processes of Life

Preprints discussed at the 2019 edition of Seeing is Believing, at EMBL Heidelberg from the 9th-12th October 2019

 



List by Dey Lab

Autophagy

Preprints on autophagy and lysosomal degradation and its role in neurodegeneration and disease. Includes molecular mechanisms, upstream signalling and regulation as well as studies on pharmaceutical interventions to upregulate the process.

 



List by Sandra Malmgren Hill

Lung Disease and Regeneration

This preprint list compiles highlights from the field of lung biology.

 



List by Rob Hynds

Cellular metabolism

A curated list of preprints related to cellular metabolism at Biorxiv by Pablo Ranea Robles from the Prelights community. Special interest on lipid metabolism, peroxisomes and mitochondria.

 



List by Pablo Ranea Robles

BSCB/BSDB Annual Meeting 2019

Preprints presented at the BSCB/BSDB Annual Meeting 2019

 



List by Dey Lab

MitoList

This list of preprints is focused on work expanding our knowledge on mitochondria in any organism, tissue or cell type, from the normal biology to the pathology.

 



List by Sandra Franco Iborra

ASCB/EMBO Annual Meeting 2018

This list relates to preprints that were discussed at the recent ASCB conference.

 



List by Dey Lab, Amanda Haage
Close