Sister-chromatid-sensitive Hi-C reveals the conformation of replicated human chromosomes
Posted on: 9 June 2020 , updated on: 11 June 2020
Preprint posted on 11 March 2020
Article now published in Nature at http://dx.doi.org/10.1038/s41586-020-2744-4
Categories: cell biology, genomics, molecular biology
Background
A large body of work has highlighted the role of structural organization of the chromosomes in regulating biological processes using chromosome conformation capture techniques 1–3. Genome folding maps have provided unprecedented insights into how intra-molecular contacts between functional genomic elements shape the formation of topologically associated domains and transcriptional profiles in development and disease contexts. How this 3D genome architecture is rebuilt during each cell-cycle with extraordinary precision has also been studied 4,5. However, existing Hi-C techniques have failed to distinguish the topological interactions between sister chromatids of replicated chromosomes due to identical DNA sequences. In this preprint, the authors have devised an ingenious technique of labeling sister chromatids to study the inter and intra- molecular interactions of replicated chromosomes from G2 to M to the following G1 phase.
Key Findings:
Development of Scs-HiC
In this preprint, the authors introduced a sister chromatid specific label by culturing the cells (HeLa) in 4sT (4 thio-thymidine) – a DNA analog of 4 thio-uridine that is routinely used for labeling nascent RNAs. Following a similar principle, sister chromatid sensitive or scs-HiC involves labeling the cells for a round of synchronized DNA replication; next, Hi-C is performed and the 4sT label is converted to 5 methyl cytosine (5mC) by OsO4 / NH4Cl DNA extraction that allows the identification of generated signature mutations by high-throughput sequencing. Double -labeled reads (4sT specific mutation on both fragments of a pairwise contact) were classified as cis sister contacts if the label mapped to the same chromosomal DNA strands on both sides and trans if it mapped to different chromosomal strands on each side. Cells were analyzed progressing from G2 to M to G1 phase.
As predicted, upon mitotic entry as the sister chromatids resolve, the trans sister contacts dropped by 2-fold (23.7 % in G2 to 9.8% in M). In G phase, trans sister contacts should disappear as the daughter cell inherits only one labeled sister chromatid per homologous chromosome. Scs Hi-C showed the trans contacts were reduced to 2.7% in G1 phase, indicating that this technique can indeed enable mapping of sister chromatid interactions.
Conformations of sister chromatids at different phases of the cell-cycle
The authors go on to generate ScsHi-C maps that show TAD organization in the replicated chromosomes. In G2 phase, there are more trans sister chromatid contacts in comparison to the prometaphase TADs. This is in concordance with the fact that during interphase, replicated chromosomes contact each other to facilitate homology-dependent DNA repair and go on to disentangle as the cells proceed into mitosis. These trans sister chromatid contacts are highly structured and are enriched at the TAD boundaries. Cis sister contacts are enriched along the diagonal within the TADs mediating short range intra-molecular contacts. Trans sister contact frequency was used to define highly paired and unpaired TADs. Highly paired TADs are enriched for H3K27me3 marked facultative heterochromatin.
Molecular regulators of sister chromatid topologies
To study the role of two functionally distinct cohesin complexes 6,7, cohesin that forms chromatin loops and cohesin responsible for sister chromatid cohesion, the cohesin loading factor that maintains cis-chromatin loops NIPBL and sister chromatid cohesion master regulator Soronin were depleted using auxin-inducible degron system at G2 phase. In NIPBL AID condition, cis sister contacts were substantially reduced but trans sister contacts were now abundant along the entire TADs and not so much at the boundaries. This indicates that this pool of cohesin is required for maintaining intra-chromatid loops, thereby separating sister chromatids at the TADs while locally enriching the chromatid cohesion at the TAD borders. On the other hand, Soronin AID results in reduction of trans sister contacts and loss of sister chromatid cohesion while the cis sister contacts remain comparable to the wild type cells. Thus, Soronin does not facilitate inter chromatid contacts but ensures that the sister chromatids are aligned during G2.
Why I chose this preprint?
The principle of Scs Hi-C is simple and elegant. It can provide us with critical insights into how the replicated chromosomes behave during the cell cycle. In this preprint the authors have looked at the role of two essential factors, NIPBL and Soronin. However, future studies using this technique can unravel the role of other key molecular players involved in this process. I like the fact that this study brings to light the intricacies and differences of cis and trans sister chromatid contacts which so far have not been distinguishable. Beyond reporting their findings, the authors have also elaborated on the validation of the robustness of the technique which is a great resource for the community.
Future directions
Scs Hi-C has the potential to provide insights into many unanswered questions in the field of genome architecture. Future investigations shall provide insights into why certain regions are more prone to sister chromatid pairing than others and how does that dictate disease context involving chromosomal re-arrangements. Additionally, we shall also learn more about how chromatids resolve themselves as the cell enters mitosis. Scs Hi-C can be a great tool to study chromosome conformation in cases of aneuploidy, for example how chromosome interactions or TADs change in conditions like Down Syndrome.
Questions for the authors:
Have the authors looked deeper into the regions that participate in trans contacts of highly paired TADs? Could these regions be involved in mitotic bookmarking?
Is there any underlying sequence feature that promotes trans sister contacts?
What is the possible correlation between H3K37me3 and highly paired TADs? Also are highly paired TAD have any correlation with the gene density of the chromosomes.
Can the authors comment on the role of architectural proteins like CTCF if any in maintaining these sister contacts in the G2 phase?
References
- Dekker, J., Rippe, K., Dekker, M. & Kleckner, N. Capturing chromosome conformation. Science (80-. ). (2002) doi:10.1126/science.1067799.
- Lieberman-Aiden, E. et al. Comprehensive mapping of long-range interactions reveals folding principles of the human genome. Science (80-. ). (2009) doi:10.1126/science.1181369.
- Dixon, J. R. et al. Topological domains in mammalian genomes identified by analysis of chromatin interactions. Nature 485, 376–380 (2012).
- Naumova, N. et al. Organization of the mitotic chromosome. Science 342, 948–53 (2013).
- Zhang, H. et al. Chromatin structure dynamics during the mitosis-to-G1 phase transition. Nature (2019) doi:10.1038/s41586-019-1778-y.
- Schwarzer, W. et al. Two independent modes of chromatin organization revealed by cohesin removal. Nature 551, 51–56 (2017).
- Rankin, S., Ayad, N. G. & Kirschner, M. W. Sororin, a substrate of the anaphase- promoting complex, is required for sister chromatid cohesion in vertebrates. Mol. Cell (2005) doi:10.1016/j.molcel.2005.03.017.
doi: https://doi.org/10.1242/prelights.21787
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