Menu

Close

F-actin patches nucleated on chromosomes coordinate capture by microtubules in oocyte meiosis

Mariia Burdyniuk, Andrea Callegari, Masashi Mori, Francois Nedelec, Peter Lenart

Preprint posted on February 13, 2018 https://www.biorxiv.org/content/early/2018/02/13/265009

When actin buys you time - a new preprint shows that large oocytes assemble actin on chromosomes to postpone their capture by microtubules. This gives them enough time to transport all of their chromosomes to the spindle before they are segregated.

Selected by Binyam Mogessie

Background. Chromosome segregation is conserved throughout eukaryotes – a spindle machinery that is assembled from microtubules aligns and equally partitions chromosomes into daughter cells. When disintegration of the nucleus at the start of cell division releases its contents into the cytoplasm, microtubules promptly capture the chromosomes. However, microtubules are not always capable of collecting all the chromosomes for segregation. For example, in large starfish oocytes, a contractile network of actin has to first deliver the chromosomes to microtubules before they can be segregated.

Key findings. How actin-driven collection of scattered chromosomes is coordinated with their capture by microtubules has remained unclear. Burdyniuk et al. now show that in starfish oocytes, actin shields chromosomes from premature attachment to microtubules until all chromosomes have been collected near the assembling spindle. Once all chromosomes have been transported, actin patches on the chromosomes are disassembled to allow their attachment to microtubules. This actin-dependent delay in chromosome capture ensures that no chromosome is left behind when segregation begins – in essence, it buys these large cells enough time to gather even those chromosomes that have scattered farthest away from probing microtubule plus ends.

What I like about this work. My own lab is interested in actin-dependent mechanisms of chromosome segregation in mammalian oocytes – we recently showed that actin filaments prevent aneuploidy in mouse eggs by efficiently bundling spindle microtubules. We have since set out on a quest to discover whether chromosome-mediated assembly of actin filaments reinforces the spindle machinery of mammalian eggs. The revelation that actin is nucleated on starfish oocyte chromosomes particularly strengthens our hypothesis, and indeed we may not be far from the truth. However, I have not selected this preprint solely because it complements our own studies. The work of Burdyniuk et al. goes well beyond confirming suspicions: it defines a previously unknown function of actin in preventing untimely chromosome-microtubule interactions that could otherwise lead to aneuploidy in eggs.

Future directions. Some immediate questions that deserve deeper mechanistic investigation arise from the work of Burdyniuk and colleagues. It remains unclear which mechanisms sense completion of chromosome gathering and selectively disassemble chromosome-associated actin filaments. The authors propose steric blockage of microtubule-chromosome interactions as one possible mechanism for delaying capture. Could actin also accomplish this task by directly binding the chromosomes’ kinetochores to render microtubule binding sites inaccessible? Finally, it will be important to examine whether this mechanism of ensuring timely chromosome-microtubule attachment is conserved in oocytes of other species, including mice and humans.

Tags: actin, aneuploidy, cell division, chromosome segregation, kinetochore, meiosis, microtubule, oocyte

Read preprint (No Ratings Yet)




  • Have your say

    Your email address will not be published. Required fields are marked *

    Sign up to customise the site to your preferences and to receive alerts

    Register here

    Also in the cell biology category:

    Rearing temperature and fatty acid supplementation jointly affect membrane fluidity and heat tolerance in Daphnia

    Dominik Martin-Creuzburg, Bret L. Coggins, Dieter Ebert, et al.



    Selected by Alexander Little

    Live-cell imaging of marked chromosome regions reveals dynamics of mitotic chromosome resolution and compaction

    John K Eykelenboom, Marek Gierlinski, Zuojun Yue, et al.

    AND

    Quantitative imaging of chromatin decompaction in living cells

    Elisa Dultz, Roberta Mancini, Guido Polles, et al.



    Selected by Carmen Adriaens, Gautam Dey

    Optogenetic reconstitution reveals that Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling forces as a multi-arm ensemble

    Masako Okumura, Toyoaki Natsume, Masato T Kanemaki, et al.



    Selected by Arnaud Monnard

    1

    Optogenetic reconstitution reveals that Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling forces as a multi-arm ensemble

    Masako Okumura, Toyoaki Natsume, Masato T Kanemaki, et al.



    Selected by Ben Craske, Thibault Legal and Toni McHugh

    A novel microtubule nucleation pathway for meiotic spindle assembly in oocytes

    Pierre ROME, Hiroyuki OHKURA



    Selected by Binyam Mogessie

    ERM proteins: The missing actin linkers in clathrin-mediated endocytosis

    Audun Sverre Kvalvaag, Kay Oliver Schink, Andreas Brech, et al.



    Selected by Nicola Stevenson

    Cell type-specific interchromosomal interactions as a mechanism for transcriptional diversity

    Adan Horta, Kevin Monahan, Lisa Bashkirova, et al.



    Selected by Boyan Bonev

    A non-canonical role for dynamin-1 in regulating early stages of clathrin-mediated endocytosis in non-neuronal cells

    Saipraveen Srinivasan, Christoph J. Burckhardt, Madhura Bhave, et al.



    Selected by Penelope La-Borde

    Atomic model of microtubule-bound tau

    Elizabeth H Kellogg, Nisreen M.A. Hejab, Simon Poepsel, et al.



    Selected by Satish Bodakuntla

    1

    Spatial Self-Organization Resolves Conflicts Between Individuality and Collective Migration

    Xiongfei Fu, Setsu Kato, Junjiajia Long, et al.



    Selected by Amanda Haage

    1

    PDX Finder: A Portal for Patient-Derived tumor Xenograft Model Discovery

    Nathalie Conte, Jeremy Mason, Csaba Halmagyi, et al.



    Selected by Carmen Adriaens

    Two contractile pools of actomyosin distinctly load and tune E-cadherin levels during morphogenesis

    Girish R. Kale, Xingbo Yang, Jean-Marc Philippe, et al.



    Selected by Arnaud Monnard

    HIF1-alpha expressing cells induce a hypoxic-like response in neighbouring cancer cells

    Hannah Harrison, Henry J Pegg, Jamie Thompson, et al.



    Selected by Anh Hoang Le

    Higher-Order Organization Principles of Pre-translational mRNPs

    Mihir Metkar, Hakan Ozadam, Bryan R. Lajoie, et al.



    Selected by Carmen Adriaens

    Capturing the onset of PRC2-mediated repressive domain formation

    Ozgur Oksuz, Varun Narendra, Chul-Hwan Lee, et al.



    Selected by Boyan Bonev

    RNA-directed activation of cytoplasmic dynein-1 in reconstituted transport RNPs

    Mark A McClintock, Carly I Dix, Christopher M Johnson, et al.

    AND

    Recruitment of Two Dyneins to an mRNA-Dependent Bicaudal D Transport Complex

    Thomas E. Sladewski, Neil Billington, M. Yusuf Ali, et al.



    Selected by Dmitry Nashchekin

    Also in the developmental biology category:

    Rearing temperature and fatty acid supplementation jointly affect membrane fluidity and heat tolerance in Daphnia

    Dominik Martin-Creuzburg, Bret L. Coggins, Dieter Ebert, et al.



    Selected by Alexander Little

    A SoxB gene acts as an anterior gap gene and regulates posterior segment addition in the spider Parasteatoda tepidariorum

    Christian L. B. Paese, Anna Schoenauer, Daniel J. Leite, et al.



    Selected by Erik Clark

    1

    Cell type-specific interchromosomal interactions as a mechanism for transcriptional diversity

    Adan Horta, Kevin Monahan, Lisa Bashkirova, et al.



    Selected by Boyan Bonev

    Germ layer specific regulation of cell polarity and adhesion gives insight into the evolution of mesoderm.

    Miguel Salinas-Saavedra, Amber Q. Rock, Mark Q. Martindale



    Selected by ClaireS & SophieM

    1

    Wnt/β-catenin regulates an ancient signaling network during zebrafish scale development

    Andrew J Aman, Alexis N Fulbright, David M Parichy



    Selected by Andreas van Impel

    Tissue flow induces cell shape changes during organogenesis

    Gonca Erdemci-Tandogan, Madeline J.Clark, Jeffrey D. Amack, et al.



    Selected by Jacky G. Goetz

    Temporal Control of Transcription by Zelda in living Drosophila embryos

    Jeremy Dufourt, Antonio Trullo, Jennifer Hunter, et al.



    Selected by Teresa Rayon

    1

    An atlas of silencer elements for the human and mouse genomes

    Naresh Doni Jayavelu, Ajay Jajodia, Arpit Mishra, et al.



    Selected by Rafael Galupa

    1

    Genetically regulated human NODAL splice variants are differentially post-transcriptionally processed and functionally distinct

    Scott D Findlay, Olena Bilyk, Kiefer Lypka, et al.



    Selected by Pierre Osteil

    Long-range Notch-mediated tissue patterning requires actomyosin contractility

    Ginger Hunter, Li He, Norbert Perrimon, et al.



    Selected by Yara E. Sánchez Corrales

    PIN7 auxin carrier is a terminator of radial root expansion in Arabidopsis thaliana

    Michel Ruiz Rosquete, Jurgen Kleine-Vehn

    AND

    PILS6 is a temperature-sensitive regulator of nuclear auxin input and organ growth in Arabidopsis thaliana

    Elena Feraru, Mugurel I. I Feraru, Elke Barbez, et al.



    Selected by Erin Sparks

    Bioelectric-calcineurin signaling module regulates allometric growth and size of the zebrafish fin

    Jacob Daane, Jennifer Lanni, Ina Rothenberg, et al.



    Selected by Alberto Rosello-Diez

    1

    Long-term live imaging of the Drosophila adult midgut reveals real-time dynamics of cell division, differentiation, and loss

    Judy Martin, Erin Nicole Sanders, Paola Moreno-Roman, et al.



    Selected by Natalie Dye

    From spiral cleavage to bilateral symmetry: The developmental cell lineage of the annelid brain

    Pavel Vopalensky, Maria Antonietta Tosches, Kaia Achim, et al.



    Selected by Erik Clark

    A 3D model of human skeletal muscle innervated with stem cell-derived motor neurons enables epsilon-subunit targeted myasthenic syndrome studies

    Mohsen Afshar Bakooshli, Ethan S Lippmann, Ben Mulcahy, et al.



    Selected by Chris Demers

    Lineage tracing axial progenitors using Nkx1.2CreERT2 mice defines their trunk and tail contributions

    Aida Rodrigo Albors, Pamela A Halley, Kate G Storey



    Selected by Pierre Osteil

    Also in the genetics category:

    Cancer modeling by Transgene Electroporation in Adult Zebrafish (TEAZ)

    Scott J Callahan, Stephanie Tepan, Yan M Zhang, et al.



    Selected by Hannah Brunsdon

    PDX Finder: A Portal for Patient-Derived tumor Xenograft Model Discovery

    Nathalie Conte, Jeremy Mason, Csaba Halmagyi, et al.



    Selected by Carmen Adriaens

    Bioelectric-calcineurin signaling module regulates allometric growth and size of the zebrafish fin

    Jacob Daane, Jennifer Lanni, Ina Rothenberg, et al.



    Selected by Alberto Rosello-Diez

    1

    Long-term live imaging of the Drosophila adult midgut reveals real-time dynamics of cell division, differentiation, and loss

    Judy Martin, Erin Nicole Sanders, Paola Moreno-Roman, et al.



    Selected by Natalie Dye

    F-actin patches nucleated on chromosomes coordinate capture by microtubules in oocyte meiosis

    Mariia Burdyniuk, Andrea Callegari, Masashi Mori, et al.



    Selected by Binyam Mogessie

    Comprehensive characterization of transcript diversity at the human NODAL locus

    Scott D Findlay, Lynne-Marie Postovit



    Selected by Christian Ramos

    Cell-nonautonomous local and systemic responses to cell arrest enable long-bone catch-up growth

    Alberto Rosello-Diez, Linda Madisen, Sebastien Bastide, et al.



    Selected by Natalie Dye

    Precise temporal regulation of alternative splicing during neural development

    Sebastien M Weyn-Vanhentenryck, Huijuan Feng, Dmytro Ustianenko, et al.



    Selected by James Gagnon

    Mutations in Bcl9 and Pygo genes cause congenital heart defects by tissue-specific perturbation of Wnt/β-catenin signaling

    Claudio Cantù, Anastasia Felker, Dario Zimmerli, et al.



    Selected by Andreas van Impel

    Insect wings and body wall evolved from ancient leg segments

    Heather S Bruce, Nipam H Patel

    AND

    Two sets of wing homologs in the crustacean, Parhyale hawaiensis

    Courtney M Clark-Hachtel, Yoshinori Tomoyasu



    Selected by Erik Clark

    2

    Close

    We want to make our website, and the services we provide, useful and reliable. This sometimes involves placing small amounts of information called cookies on the device you used to access the internet. If you continue to use this website we will assume you are happy to accept our cookies.

    Accept