Close

Glioblastoma extracellular vesicles influence glial cell hyaluronic acid deposition to promote invasiveness

Dominik Koessinger, David Novo, Anna Koessinger, America Campos, Jasmine Peters, Louise Dutton, Peggy Paschke, Désirée Zerbst, Madeleine Moore, Louise Mitchell, Matthew Neilson, Katrina Stevenson, Anthony Chalmers, Stephen Tait, Joanna Birch, Jim Norman

Posted on: 13 June 2023 , updated on: 11 August 2023

Preprint posted on 11 May 2023

How to use your neighbours to get ahead: brain tumour cells use extracellular vesicles to encourage neighbouring astrocytes to secrete pro-invasive extracellular matrix components.

Selected by Jade Chan

Introduction

Glioblastoma (GBM) is a highly aggressive and invasive brain tumour that inevitably recurs following standard-of-care treatments. GBM cells that infiltrate into healthy brain tissues beyond surgical margins are often the source of recurrent tumours1. Prior studies have revealed that tumour cells migrate in an adhesion-dependent manner along blood vessels and white matter tracts. GBM cells also exhibit extensive crosstalk with cells present in their microenvironment: for example, they can form interconnected networks via tunneling nanotubes as well as bona fide excitatory synapses with surrounding neurons2-5. Tumour cells can also influence neighbouring cells by secreting extracellular vesicles (EVs). For instance, tumour cell derived EVs have been demonstrated to promote an immune-suppressive environment to bolster tumour growth6-9. To prevent recurrence and improve patient outcomes, there is an urgent need to investigate how crosstalk between GBM cells and their neighbours fosters GBM cell invasion.

In this study, the authors illustrate a pro-invasive signalling axis between GBM cells and neighbouring astrocytes. GBM cells bearing an oncogenic p53R273H mutation release EVs containing podocalyxin into their microenvironment, which are picked up by astrocytes. In response, astrocytes increase their deposition of hyaluronic acid (HA)-rich extracellular matrix, providing a substrate for boosting GBM cell migration. Importantly, the authors demonstrate that genetic deletion of PODXL in GBM cells attenuates GBM cell invasion in vivo.

Key Findings

EVs from GBM cells influence astrocytes to deposit ECM

The authors used two patient-derived glioma stem-cell like lines (G7 and E2 cells) that exhibited different invasive characteristics in vivo. E2 cells infiltrate throughout the brain in a scattered manner, whereas G7 cells grow as a solid tumour mass with little invasion. The authors hypothesized that factors secreted by GBM cells could underlie their different migratory patterns through the ECM of the brain, which is primarily maintained by astrocytes. Therefore, they harvested EVs secreted by G7 and E2 cells, treated primary astrocyte cultures with EVs, then allowed astrocytes to deposit ECM. Interestingly, GBM cells that were plated onto ECM deposited by astrocytes treated with EVs from E2 cells migrated much more quickly than those treated with EVs from G7 cells, hinting that the composition of G7 versus E2-derived EVs differed in their ability to influence ECM deposition by astrocytes.

Deleting p53R273H from GBM cells reduces ECM deposition from astrocytes and decreases GBM cell migration

Deep sequencing revealed that highly invasive E2 cells bear an oncogenic p53R273H mutation. Previous studies showed that the p53R273H mutation in carcinoma cells promotes cell migration by controlling the amount of podocalyxin (PODXL) in tumour-cell derived EVs10. After deleting p53R273H in E2 cells using CRISPR-Cas9, the authors found PODXL levels within EVs were increased in p53R273H KO E2 cells, and EVs derived from these cells had a decreased ability to promote ECM deposition from astrocytes. The authors hypothesized that PODXL levels within EVs must fall within a specific range to encourage astrocytes to deposit pro-migratory ECM. To verify this, they generated PODXL-overexpressing (OE) cells or deleted the PODXL gene in E2 cells (PODXL-KO). Astrocyte cultures treated with EVs from PODXL-OE or KO E2 cells did not deposit ECM in a way that supported GBM cell migration as observed via live cell imaging. Furthermore, pre-treatment of mouse brain slices with PODXL-OE or KO EVs decreased the migratory capacity of GBM cells seeded onto the slices, suggesting the level of PODXL within EVs must be finely tuned.

EVs promote GBM cell migration by tuning hyaluronic acid content of astrocyte-derived ECM

The ECM within the brain has a unique composition of proteo- and glycosamino-glycans which differs from fibrillar proteins that are typically found in the ECM of other organs11. To determine how GBM-cell derived EVs influence the composition of ECM deposited by astrocytes, the authors performed a screen using a panel of lectins (proteins that bind carbohydrates) and other reagents that bind carbohydrate moieties. Notably, treating astrocytes with EVs from p53R273H E2 cells increased the hyaluronic acid (HA) content in the ECM, as detected by hyaluronic acid binding protein (HABP) and immunofluorescence staining. HA levels were unaffected when astrocytes were treated with EVs from G7, p53R273H-KO E2, or PODXL-KO E2 cells. To determine whether HA was responsible for boosting GBM cell migration, the authors treated astrocyte cultures with hyaluronidase (Hase), an enzyme that degrades HA. Hase treatment significantly decreased the migratory capacity of GBM cells, indicating that HA is a key ECM component that promotes the invasion of GBM cells.

PODXL drives mutant p53R273H-driven infiltrative behaviour of GBM in vivo

To determine whether EV-mediated crosstalk between GBM cells and astrocytes promotes tumour cell invasion in vivo, the authors injected p53R273H-KO E2, PODXL-KO E2 cells, or their control counterparts in the right forebrain of immunocompromised mice. p53R273H-KO cells generated tumours that were too small to quantify invasive characteristics. In contrast, while PODXL-KO did not affect the overall proportion of proliferating (Ki67+) tumour cells, a much smaller proportion of GBM cells migrated to the left hemisphere of the brain compared to control, indicating their invasive capacity was compromised. Overall, these results suggest that targeting PODXL is an effective strategy for curbing GBM cell invasion.

Figure 1. A schematic outlining the EV-mediated crosstalk between GBM cells and astrocytes. GBM cells bearing the p53R273H mutation secrete PODXL-containing EVs into their extracellular environment. Astrocytes respond to EVs by increasing HA deposition. Increased HA content in the ECM promotes GBM cell migration and infiltration into distant brain tissues. Schematic drawn by Reinier Prosée.

Why I chose this preprint

GBM is notoriously infiltrative, which contributes to its near-universal recurrence following surgery. I chose this preprint because it illustrates a complete signalling axis wherein GBM cells influence their neighbouring cell types into fuelling their invasion into healthy brain tissue. I am currently investigating the role of a specific protein in adhesion-dependent versus adhesion-independent migration in GBM. This preprint, along with other studies examining the crosstalk between tumour cells and their microenvironment, have inspired me to look beyond cell-autonomous factors that influence GBM cell invasion. Looking into microenvironmental relationships may be important for developing new therapies since GBM cells are highly heterogeneous.

Questions for the authors

  1. Is the overall survival of the mice bearing PODXL-KO xenograft tumours prolonged compared to control?
  2. How does EV-derived PODXL boost HA secretion in astrocytes?
  3. Surprisingly, EVs derived from p53R273H-KO E2 cells contained a greater amount of PODXL, yet their ability to encourage astrocytes to deposit pro-migratory ECM was reduced. How does p53R273H tune the amount of PODXL present in EVs?
  4. Can other cells in the GBM microenvironment (ex. microglia, neurons, oligodendrocytes) also perceive and respond to PODXL-containing EVs?

References

  1. Miller CR, Perry A. Glioblastoma. Arch Pathol Lab Med. 2007; 131(3):397-406.
  2. Osswald M, Jung E, Sahm F, et al. Brain tumour cells interconnect to a functional and resistant network. Nature. 2015; 528(7580):93-98.
  3. Pinto G, Saenz-de-Santa-Maria I, Chastagner P, et al. Patient-derived glioblastoma stem cells transfer mitochondria through tunneling nanotubes in tumor organoids. Biochem J. 2021; 478(1):21-39.
  4. Venkatesh, H. S., Morishita, W., Geraghty, A. C., Silverbush, D., Gillespie, S. M., Arzt, M., Tam, L. T., Espenel, C., Ponnuswami, A., Ni, L., Woo, P. J., Taylor, K. R., Agarwal, A., Regev, A., Brang, D., Vogel, H., Hervey-Jumper, S., Bergles, D. E., Suvà, M. L., Malenka, R. C., … Monje, M. Electrical and synaptic integration of glioma into neural circuits. Nature. 2019; 573(7775), 539–545.
  5. Venkataramani, V., Tanev, D. I., Strahle, C., Studier-Fischer, A., Fankhauser, L., Kessler, T., Körber, C., Kardorff, M., Ratliff, M., Xie, R., Horstmann, H., Messer, M., Paik, S. P., Knabbe, J., Sahm, F., Kurz, F. T., Acikgöz, A. A., Herrmannsdörfer, F., Agarwal, A., Bergles, D. E., … Kuner, T. Glutamatergic synaptic input to glioma cells drives brain tumour progression. Nature. 2019; 573(7775), 532–538.
  6. de Vrij J, Maas SL, Kwappenberg KM, et al. Glioblastoma-derived extracellular vesicles modify the phenotype of monocytic cells. Int J Cancer. 2015; 137(7):1630-1642.
  7. Domenis R, Cesselli D, Toffoletto B, et al. Systemic T Cells Immunosuppression of Glioma Stem Cell-Derived Exosomes Is Mediated by Monocytic Myeloid-Derived Suppressor Cells. PLoS One. 2017; 12(1):e0169932.
  8. Gabrusiewicz K, Li X, Wei J, et al. Glioblastoma stem cell-derived exosomes induce M2 macrophages and PD-L1 expression on human monocytes. Oncoimmunology. 2018; 7(4):e1412909.
  9. Hellwinkel JE, Redzic JS, Harland TA, Gunaydin D, Anchordoquy TJ, Graner MW. Glioma-derived extracellular vesicles selectively suppress immune responses. Neuro Oncol. 2016; 18(4):497-506.
  10. Novo D, Heath N, Mitchell L, et al. Mutant p53s generate pro-invasive niches by influencing exosome podocalyxin levels. Nat Commun. 2018; 9(1):5069.
  11. Ruoslahti E. Brain extracellular matrix. Glycobiology. 1996; 6(5):489-492

Tags: astrocyte, extracellular matrix, glioblastoma, glioma, migration, vesicle

doi: https://doi.org/10.1242/prelights.34825

Read preprint (No Ratings Yet)

Author's response

The author team shared

Is the overall survival of the mice bearing PODXL-KO xenograft tumours prolonged compared to control?

We have, so far, performed timed endpoint experiments to allow us to accurately assess the extent to which PODXL-KO in the primary tumour influences its infiltrative capacity.  We currently do not know whether the reduced infiltrative capacity of PODXL-KO cells would alter survival times.  Indeed, this is difficult to predict from what is currently known.  The symptoms of GBM result from pressure exerted by the bulk of the primary tumour and its associated oedema.  However, there is also evidence that infiltrating cells can increase morbidity, particularly if they interfere with brain stem function.  Moreover, there is evidence that infiltrating GBM cells can affect cognitive function.  Further experimentation will be required to address these questions.

How does EV-derived PODXL boost HA secretion in astrocytes?

We currently know that astrocytes must express the lipid kinase, DGK-alpha to deposit migration-promoting ECM in response to EVs from mp53-expressing glioma.  We have performed several previous studies indicating that pro-invasive phenotypes evoked by mutant p53s depend on DGK-alpha because this kinase is required for controlling membrane trafficking pathways carrying endosomal recycling vesicles to the plasma membrane.  We, therefore, propose that DGK-alpha mediates responses to mutant p53 EVs in astrocytes by controlling membrane trafficking events which influence HA deposition. Previous studies have indicated that the activity of HA synthases can be influenced by how they are trafficked to and from the plasma membrane (these are cited in our discussion), and further investigations into the endosomal trafficking of these enzymes will, hopefully, address this question.

Surprisingly, EVs derived from p53R273H-KO E2 cells contained a greater amount of PODXL, yet their ability to encourage astrocytes to deposit pro-migratory ECM was reduced. How does p53R273H tune the amount of PODXL present in EVs?

Yes, we have found that, in several systems (including non-small cell lung cancer cell lines, patient-derived pancreatic cancer lines, mouse GEMMs of pancreatic cancer and patient-derived and established GBM lines) that p53-273H leads to reduced PODXL levels in EVs.  Moreover, we have found that this moves EV PODXL levels into a range in which these EVs can influence ECM deposition by recipient cells.  We call this the ‘Goldilocks’ range.  If one moves PODXL out of this range, by increasing (by overexpression) or decreasing it (by knockdown/knockout), EVs from p35-273H-expressing cells lose the ability to influence ECM deposition.  In pancreatic and lung cancer cells we know that p53-273H reduces transcription of PODXL by suppressing the activity of p63, another p53 family member.  Also, Rab35 activity is important for sorting PODXL between the plasma membrane and late endosomes/EVs.  However, in GBM the situation is less clear.  This is because, unlike pancreatic and lung cancer cells, cellular PODXL seem to be unchanged by altering p53-273H -expression in GBM lines.  Thus, we hypothesise that, in GBM, p53-273H can alter Rab35-dependent sorting of PODXL to EVs and further work will be needed to resolve this.

Can other cells in the GBM microenvironment (ex. microglia, neurons, oligodendrocytes) also perceive and respond to PODXL-containing EVs?

We have not investigated this so far.  We studied astrocytes, as these are the main producer/depositors of ECM in the brain.  However, other glial cells, particularly oligodendrocytes, synthesize and deposit ECM, and microglia (being macrophage-like) have a key role in ECM remodelling in the brain.  Finally, as discussed in our paper, it is probable that incoming myeloid cells (such as neutrophils) are likely to be influenced by GBM-derived EVs and/or by altered HA levels in the brain.  We will be planning future investigations into the influence that GBM EVs and altered HA levels have on the behaviour of incoming neutrophils, and how this may influence GBM growth, invasion angiogenesis and responses to immunotherapies.

Have your say

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

Sign up to customise the site to your preferences and to receive alerts

Register here

Also in the cancer biology category:

Integrin conformation-dependent neutrophil slowing obstructs the capillaries of the pre-metastatic lung in a model of breast cancer

Frédéric Fercoq, Gemma S. Cairns, Marco De Donatis, et al.

Selected by 07 October 2024

Simon Cleary

Cancer Biology

Mitochondria-derived nuclear ATP surge protects against confinement-induced proliferation defects

Ritobrata Ghose, Fabio Pezzano, Savvas Kourtis, et al.

Selected by 16 May 2024

Teodora Piskova

Cell Biology

Spatial transcriptomics elucidates medulla niche supporting germinal center response in myasthenia gravis thymoma

Yoshiaki Yasumizu, Makoto Kinoshita, Martin Jinye Zhang, et al.

Selected by 27 March 2024

Jessica Chevallier

Immunology

Also in the cell biology category:

The RNA binding protein HNRNPA2B1 regulates RNA abundance and motor protein activity in neurites

Joelle Lo, Katherine F. Vaeth, Gurprit Bhardwaj, et al.

Selected by 24 September 2024

Felipe Del Valle Batalla

Neuroscience

Pharyngeal neuronal mechanisms governing sour taste perception in Drosophila melanogaster

Bhanu Shrestha, Jiun Sang, Suman Rimal, et al.

Selected by 23 September 2024

Matthew Davies

Cell Biology

Feedback regulation by the RhoA-specific GEF ARHGEF17 regulates actomyosin network disassembly

Vasundhara Rao, Benjamin Grädel, Lucien Hinderling, et al.

Selected by 18 September 2024

Vibha SINGH

Cell Biology

Also in the neuroscience category:

The RNA binding protein HNRNPA2B1 regulates RNA abundance and motor protein activity in neurites

Joelle Lo, Katherine F. Vaeth, Gurprit Bhardwaj, et al.

Selected by 24 September 2024

Felipe Del Valle Batalla

Neuroscience

Pharyngeal neuronal mechanisms governing sour taste perception in Drosophila melanogaster

Bhanu Shrestha, Jiun Sang, Suman Rimal, et al.

Selected by 23 September 2024

Matthew Davies

Cell Biology

Triglyceride metabolism controls inflammation and APOE4-associated disease states in microglia

Roxan A. Stephenson, Kory R. Johnson, Linling Cheng, et al.

Selected by 22 August 2024

Gustavo Stelzer, Marcus Oliveira

Biochemistry

preLists in the cancer biology category:

BSCB-Biochemical Society 2024 Cell Migration meeting

This preList features preprints that were discussed and presented during the BSCB-Biochemical Society 2024 Cell Migration meeting in Birmingham, UK in April 2024. Kindly put together by Sara Morais da Silva, Reviews Editor at Journal of Cell Science.

 



List by Reinier Prosee

CSHL 87th Symposium: Stem Cells

Preprints mentioned by speakers at the #CSHLsymp23

 



List by Alex Eve

Journal of Cell Science meeting ‘Imaging Cell Dynamics’

This preList highlights the preprints discussed at the JCS meeting 'Imaging Cell Dynamics'. The meeting was held from 14 - 17 May 2023 in Lisbon, Portugal and was organised by Erika Holzbaur, Jennifer Lippincott-Schwartz, Rob Parton and Michael Way.

 



List by Helen Zenner

CellBio 2022 – An ASCB/EMBO Meeting

This preLists features preprints that were discussed and presented during the CellBio 2022 meeting in Washington, DC in December 2022.

 



List by Nadja Hümpfer et al.

Fibroblasts

The advances in fibroblast biology preList explores the recent discoveries and preprints of the fibroblast world. Get ready to immerse yourself with this list created for fibroblasts aficionados and lovers, and beyond. Here, my goal is to include preprints of fibroblast biology, heterogeneity, fate, extracellular matrix, behavior, topography, single-cell atlases, spatial transcriptomics, and their matrix!

 



List by Osvaldo Contreras

Single Cell Biology 2020

A list of preprints mentioned at the Wellcome Genome Campus Single Cell Biology 2020 meeting.

 



List by Alex Eve

ASCB EMBO Annual Meeting 2019

A collection of preprints presented at the 2019 ASCB EMBO Meeting in Washington, DC (December 7-11)

 



List by Madhuja Samaddar et al.

Lung Disease and Regeneration

This preprint list compiles highlights from the field of lung biology.

 



List by Rob Hynds

Anticancer agents: Discovery and clinical use

Preprints that describe the discovery of anticancer agents and their clinical use. Includes both small molecules and macromolecules like biologics.

 



List by Zhang-He Goh

Biophysical Society Annual Meeting 2019

Few of the preprints that were discussed in the recent BPS annual meeting at Baltimore, USA

 



List by Joseph Jose Thottacherry

Also in the cell biology category:

BSCB-Biochemical Society 2024 Cell Migration meeting

This preList features preprints that were discussed and presented during the BSCB-Biochemical Society 2024 Cell Migration meeting in Birmingham, UK in April 2024. Kindly put together by Sara Morais da Silva, Reviews Editor at Journal of Cell Science.

 



List by Reinier Prosee

‘In preprints’ from Development 2022-2023

A list of the preprints featured in Development's 'In preprints' articles between 2022-2023

 



List by Alex Eve, Katherine Brown

preLights peer support – preprints of interest

This is a preprint repository to organise the preprints and preLights covered through the 'preLights peer support' initiative.

 



List by preLights peer support

The Society for Developmental Biology 82nd Annual Meeting

This preList is made up of the preprints discussed during the Society for Developmental Biology 82nd Annual Meeting that took place in Chicago in July 2023.

 



List by Joyce Yu, Katherine Brown

CSHL 87th Symposium: Stem Cells

Preprints mentioned by speakers at the #CSHLsymp23

 



List by Alex Eve

Journal of Cell Science meeting ‘Imaging Cell Dynamics’

This preList highlights the preprints discussed at the JCS meeting 'Imaging Cell Dynamics'. The meeting was held from 14 - 17 May 2023 in Lisbon, Portugal and was organised by Erika Holzbaur, Jennifer Lippincott-Schwartz, Rob Parton and Michael Way.

 



List by Helen Zenner

9th International Symposium on the Biology of Vertebrate Sex Determination

This preList contains preprints discussed during the 9th International Symposium on the Biology of Vertebrate Sex Determination. This conference was held in Kona, Hawaii from April 17th to 21st 2023.

 



List by Martin Estermann

Alumni picks – preLights 5th Birthday

This preList contains preprints that were picked and highlighted by preLights Alumni - an initiative that was set up to mark preLights 5th birthday. More entries will follow throughout February and March 2023.

 



List by Sergio Menchero et al.

CellBio 2022 – An ASCB/EMBO Meeting

This preLists features preprints that were discussed and presented during the CellBio 2022 meeting in Washington, DC in December 2022.

 



List by Nadja Hümpfer et al.

Fibroblasts

The advances in fibroblast biology preList explores the recent discoveries and preprints of the fibroblast world. Get ready to immerse yourself with this list created for fibroblasts aficionados and lovers, and beyond. Here, my goal is to include preprints of fibroblast biology, heterogeneity, fate, extracellular matrix, behavior, topography, single-cell atlases, spatial transcriptomics, and their matrix!

 



List by Osvaldo Contreras

EMBL Synthetic Morphogenesis: From Gene Circuits to Tissue Architecture (2021)

A list of preprints mentioned at the #EESmorphoG virtual meeting in 2021.

 



List by Alex Eve

FENS 2020

A collection of preprints presented during the virtual meeting of the Federation of European Neuroscience Societies (FENS) in 2020

 



List by Ana Dorrego-Rivas

Planar Cell Polarity – PCP

This preList contains preprints about the latest findings on Planar Cell Polarity (PCP) in various model organisms at the molecular, cellular and tissue levels.

 



List by Ana Dorrego-Rivas

BioMalPar XVI: Biology and Pathology of the Malaria Parasite

[under construction] Preprints presented at the (fully virtual) EMBL BioMalPar XVI, 17-18 May 2020 #emblmalaria

 



List by Dey Lab, Samantha Seah

1

Cell Polarity

Recent research from the field of cell polarity is summarized in this list of preprints. It comprises of studies focusing on various forms of cell polarity ranging from epithelial polarity, planar cell polarity to front-to-rear polarity.

 



List by Yamini Ravichandran

TAGC 2020

Preprints recently presented at the virtual Allied Genetics Conference, April 22-26, 2020. #TAGC20

 



List by Maiko Kitaoka et al.

3D Gastruloids

A curated list of preprints related to Gastruloids (in vitro models of early development obtained by 3D aggregation of embryonic cells). Updated until July 2021.

 



List by Paul Gerald L. Sanchez and Stefano Vianello

ECFG15 – Fungal biology

Preprints presented at 15th European Conference on Fungal Genetics 17-20 February 2020 Rome

 



List by Hiral Shah

ASCB EMBO Annual Meeting 2019

A collection of preprints presented at the 2019 ASCB EMBO Meeting in Washington, DC (December 7-11)

 



List by Madhuja Samaddar et al.

EMBL Seeing is Believing – Imaging the Molecular Processes of Life

Preprints discussed at the 2019 edition of Seeing is Believing, at EMBL Heidelberg from the 9th-12th October 2019

 



List by Dey Lab

Autophagy

Preprints on autophagy and lysosomal degradation and its role in neurodegeneration and disease. Includes molecular mechanisms, upstream signalling and regulation as well as studies on pharmaceutical interventions to upregulate the process.

 



List by Sandra Malmgren Hill

Lung Disease and Regeneration

This preprint list compiles highlights from the field of lung biology.

 



List by Rob Hynds

Cellular metabolism

A curated list of preprints related to cellular metabolism at Biorxiv by Pablo Ranea Robles from the Prelights community. Special interest on lipid metabolism, peroxisomes and mitochondria.

 



List by Pablo Ranea Robles

BSCB/BSDB Annual Meeting 2019

Preprints presented at the BSCB/BSDB Annual Meeting 2019

 



List by Dey Lab

MitoList

This list of preprints is focused on work expanding our knowledge on mitochondria in any organism, tissue or cell type, from the normal biology to the pathology.

 



List by Sandra Franco Iborra

ASCB/EMBO Annual Meeting 2018

This list relates to preprints that were discussed at the recent ASCB conference.

 



List by Dey Lab, Amanda Haage

Also in the neuroscience category:

2024 Hypothalamus GRC

This 2024 Hypothalamus GRC (Gordon Research Conference) preList offers an overview of cutting-edge research focused on the hypothalamus, a critical brain region involved in regulating homeostasis, behavior, and neuroendocrine functions. The studies included cover a range of topics, including neural circuits, molecular mechanisms, and the role of the hypothalamus in health and disease. This collection highlights some of the latest advances in understanding hypothalamic function, with potential implications for treating disorders such as obesity, stress, and metabolic diseases.

 



List by Nathalie Krauth

‘In preprints’ from Development 2022-2023

A list of the preprints featured in Development's 'In preprints' articles between 2022-2023

 



List by Alex Eve, Katherine Brown

CSHL 87th Symposium: Stem Cells

Preprints mentioned by speakers at the #CSHLsymp23

 



List by Alex Eve

Journal of Cell Science meeting ‘Imaging Cell Dynamics’

This preList highlights the preprints discussed at the JCS meeting 'Imaging Cell Dynamics'. The meeting was held from 14 - 17 May 2023 in Lisbon, Portugal and was organised by Erika Holzbaur, Jennifer Lippincott-Schwartz, Rob Parton and Michael Way.

 



List by Helen Zenner

FENS 2020

A collection of preprints presented during the virtual meeting of the Federation of European Neuroscience Societies (FENS) in 2020

 



List by Ana Dorrego-Rivas

ASCB EMBO Annual Meeting 2019

A collection of preprints presented at the 2019 ASCB EMBO Meeting in Washington, DC (December 7-11)

 



List by Madhuja Samaddar et al.

SDB 78th Annual Meeting 2019

A curation of the preprints presented at the SDB meeting in Boston, July 26-30 2019. The preList will be updated throughout the duration of the meeting.

 



List by Alex Eve

Autophagy

Preprints on autophagy and lysosomal degradation and its role in neurodegeneration and disease. Includes molecular mechanisms, upstream signalling and regulation as well as studies on pharmaceutical interventions to upregulate the process.

 



List by Sandra Malmgren Hill

Young Embryologist Network Conference 2019

Preprints presented at the Young Embryologist Network 2019 conference, 13 May, The Francis Crick Institute, London

 



List by Alex Eve
Close