Close

Two contractile pools of actomyosin distinctly load and tune E-cadherin levels during morphogenesis

Girish R. Kale, Xingbo Yang, Jean-Marc Philippe, Madhav Mani, Pierre-François Lenne, Thomas Lecuit

Posted on: 18 April 2018

Preprint posted on 28 January 2018

Article now published in Nature Communications at http://dx.doi.org/10.1038/s41467-018-07448-8

Fine tuning of adhesion complexes through two distinct pools of actomyosin

Selected by Arnaud Monnard

Categories: biophysics, cell biology

Context

In metazoans, germ-band extension is a morphogenetic process that drives body axis elongation along the anterior-posterior axis1. The extension is highly dynamic and lasts for about 2.5 hours, but most of the visible changes are concentrated during the first 45 min, called the rapid phase. This extension is thought to be mainly driven by cell intercalation2, in which planar polarization of actomyosin and interaction with E-cadherin (E-cad) at adhesion junctions play critical roles in junction remodeling3. Moreover recent work has shown that epithelial junction remodeling is achieved through the action of two pools of non-muscle myosin II (junctional or medial Myo), which are regulated separately3,4.   

 

Key findings

In this study, Kale et al. looked at the distinct interaction between adhesion complexes and medial or junctional Myo. They initially show that the polarized localization of Vinculin (Vinc) at adhesion complexes is achieved through α-Catenin and that this localization depends on Myo. Next they established a direct correlation between junctional tension and both Vinc/E-cad ratio and junctional Myo by measuring the recoil velocity of Myo and the Vinc/E-cad ratio after laser ablation of the junction. The biophysical measurements were then supplemented with non-invasive mechanical inference to deduce junctional tensions. Subsequently, the authors established that the Vinc/E-cad ratio could be used as a representative measure of the “load on adhesion complexes”.

 

Figure adapted from Kale et al. : (A) Vinc/E-cad ratio correlates with junctional tension estimated using mechanical inference. (B) Schematic representation of a model of the junction to construct shear stress and the corresponding equation.

 

Having established the Vinc/E-cad ratio as a meaningful measure of tension, they were then able to address the core focus of the paper: to determine the specific contribution of each pool of Myo (junctional and medial) to adhesion complexes. The authors achieved this by selectively reducing or overexpressing medial Myo. In doing so, they found that medial Myo was responsible for the recruitment of adhesion complexes at the junction globally, whereas junctional Myo was responsible for the planar distribution of adhesion complexes by inducing shear stress on vertical junctions.

 

Why I chose it

A strength of this study lies in its power to decipher the subcellular physical mechanisms that drive ectodermal convergent-extension movements. By looking at the specific contribution of two different pools of myosin on adhesion complexes, the authors offer us a very detailed and novel perspective on junction dynamics. Moreover, the combination of invasive (laser ablation) and non-invasive (mechanical inference, correlative analysis) measurements in an intact living Drosophila embryo allows the authors to elegantly derive physical properties (tension, stress) of the junction.

 

Future perspectives

It would be interesting to investigate if these results (the specific contribution of two different pools of Myo to adhesion complexes) could be transposed to different model systems, such as in the fly notum. Finally, and as the authors point out, it remains to be elucidated exactly how the generation of contractile forces by myosin-II activity controls junction remodeling during morphogenesis.

 

References

  1. Irvine, K. D. & Wieschaus, E. Cell intercalation during Drosophila germband extension and its regulation by pair-rule segmentation genes. Development120,827–841 (1994).
  2. Kong, D., Wolf, F. & Großhans, J. Forces directing germ-band extension in Drosophila embryos. Mech. Dev.144,11–22 (2017).
  3. Rauzi, M., Lenne, P. F. & Lecuit, T. Planar polarized actomyosin contractile flows control epithelial junction remodelling. Nature468,1110–1115 (2010).
  4. Kerridge, S. et al.Modular activation of Rho1 by GPCR signalling imparts polarized myosin II activation during morphogenesis. Nat. Cell Biol.(2016). doi:10.1038/ncb3302

Tags: mechanobiology

Read preprint (2 votes)

Have your say

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

Sign up to customise the site to your preferences and to receive alerts

Register here

Also in the biophysics category:

Topology changes of the regenerating Hydra define actin nematic defects as mechanical organizers of morphogenesis

Yamini Ravichandran, Matthias Vogg, Karsten Kruse, et al.

Selected by 08 May 2024

Rachel Mckeown

Developmental Biology

Structural basis of respiratory complexes adaptation to cold temperatures

Young-Cheul Shin, Pedro Latorre-Muro, Amina Djurabekova, et al.

Selected by 10 April 2024

Pamela Ornelas

Biophysics

Actin polymerization drives lumen formation in a human epiblast model

Dhiraj Indana, Andrei Zakharov, Youngbin Lim, et al.

Selected by 05 April 2024

Megane Rayer, Rivka Shapiro

Biophysics

Also in the cell biology category:

Mitochondria-derived nuclear ATP surge protects against confinement-induced proliferation defects

Ritobrata Ghose, Fabio Pezzano, Savvas Kourtis, et al.

Selected by 16 May 2024

Teodora Piskova

Cell Biology

Lipid-Based Transfection of Zebrafish Embryos: A Robust Protocol for Nucleic Acid Delivery

Aslihan Terzi, Tiger Liao, Adrian Jacobo

Selected by 09 May 2024

Roberto Rodríguez-Morales

Genetics

Fetal brain response to maternal inflammation requires microglia

Bridget Elaine LaMonica Ostrem, Nuria Dominguez Iturza, Jeffrey Stogsdill, et al.

Selected by 24 April 2024

Manuel Lessi

Neuroscience

Also in the cell biology category:

BSCB-Biochemical Society 2024 Cell Migration meeting

This preList features preprints that were discussed and presented during the BSCB-Biochemical Society 2024 Cell Migration meeting in Birmingham, UK in April 2024. Kindly put together by Sara Morais da Silva, Reviews Editor at Journal of Cell Science.

 



List by Reinier Prosee

‘In preprints’ from Development 2022-2023

A list of the preprints featured in Development's 'In preprints' articles between 2022-2023

 



List by Alex Eve, Katherine Brown

preLights peer support – preprints of interest

This is a preprint repository to organise the preprints and preLights covered through the 'preLights peer support' initiative.

 



List by preLights peer support

The Society for Developmental Biology 82nd Annual Meeting

This preList is made up of the preprints discussed during the Society for Developmental Biology 82nd Annual Meeting that took place in Chicago in July 2023.

 



List by Joyce Yu, Katherine Brown

CSHL 87th Symposium: Stem Cells

Preprints mentioned by speakers at the #CSHLsymp23

 



List by Alex Eve

Journal of Cell Science meeting ‘Imaging Cell Dynamics’

This preList highlights the preprints discussed at the JCS meeting 'Imaging Cell Dynamics'. The meeting was held from 14 - 17 May 2023 in Lisbon, Portugal and was organised by Erika Holzbaur, Jennifer Lippincott-Schwartz, Rob Parton and Michael Way.

 



List by Helen Zenner

9th International Symposium on the Biology of Vertebrate Sex Determination

This preList contains preprints discussed during the 9th International Symposium on the Biology of Vertebrate Sex Determination. This conference was held in Kona, Hawaii from April 17th to 21st 2023.

 



List by Martin Estermann

Alumni picks – preLights 5th Birthday

This preList contains preprints that were picked and highlighted by preLights Alumni - an initiative that was set up to mark preLights 5th birthday. More entries will follow throughout February and March 2023.

 



List by Sergio Menchero et al.

CellBio 2022 – An ASCB/EMBO Meeting

This preLists features preprints that were discussed and presented during the CellBio 2022 meeting in Washington, DC in December 2022.

 



List by Nadja Hümpfer et al.

Fibroblasts

The advances in fibroblast biology preList explores the recent discoveries and preprints of the fibroblast world. Get ready to immerse yourself with this list created for fibroblasts aficionados and lovers, and beyond. Here, my goal is to include preprints of fibroblast biology, heterogeneity, fate, extracellular matrix, behavior, topography, single-cell atlases, spatial transcriptomics, and their matrix!

 



List by Osvaldo Contreras

EMBL Synthetic Morphogenesis: From Gene Circuits to Tissue Architecture (2021)

A list of preprints mentioned at the #EESmorphoG virtual meeting in 2021.

 



List by Alex Eve

FENS 2020

A collection of preprints presented during the virtual meeting of the Federation of European Neuroscience Societies (FENS) in 2020

 



List by Ana Dorrego-Rivas

Planar Cell Polarity – PCP

This preList contains preprints about the latest findings on Planar Cell Polarity (PCP) in various model organisms at the molecular, cellular and tissue levels.

 



List by Ana Dorrego-Rivas

BioMalPar XVI: Biology and Pathology of the Malaria Parasite

[under construction] Preprints presented at the (fully virtual) EMBL BioMalPar XVI, 17-18 May 2020 #emblmalaria

 



List by Dey Lab, Samantha Seah

1

Cell Polarity

Recent research from the field of cell polarity is summarized in this list of preprints. It comprises of studies focusing on various forms of cell polarity ranging from epithelial polarity, planar cell polarity to front-to-rear polarity.

 



List by Yamini Ravichandran

TAGC 2020

Preprints recently presented at the virtual Allied Genetics Conference, April 22-26, 2020. #TAGC20

 



List by Maiko Kitaoka et al.

3D Gastruloids

A curated list of preprints related to Gastruloids (in vitro models of early development obtained by 3D aggregation of embryonic cells). Updated until July 2021.

 



List by Paul Gerald L. Sanchez and Stefano Vianello

ECFG15 – Fungal biology

Preprints presented at 15th European Conference on Fungal Genetics 17-20 February 2020 Rome

 



List by Hiral Shah

ASCB EMBO Annual Meeting 2019

A collection of preprints presented at the 2019 ASCB EMBO Meeting in Washington, DC (December 7-11)

 



List by Madhuja Samaddar et al.

EMBL Seeing is Believing – Imaging the Molecular Processes of Life

Preprints discussed at the 2019 edition of Seeing is Believing, at EMBL Heidelberg from the 9th-12th October 2019

 



List by Dey Lab

Autophagy

Preprints on autophagy and lysosomal degradation and its role in neurodegeneration and disease. Includes molecular mechanisms, upstream signalling and regulation as well as studies on pharmaceutical interventions to upregulate the process.

 



List by Sandra Malmgren Hill

Lung Disease and Regeneration

This preprint list compiles highlights from the field of lung biology.

 



List by Rob Hynds

Cellular metabolism

A curated list of preprints related to cellular metabolism at Biorxiv by Pablo Ranea Robles from the Prelights community. Special interest on lipid metabolism, peroxisomes and mitochondria.

 



List by Pablo Ranea Robles

BSCB/BSDB Annual Meeting 2019

Preprints presented at the BSCB/BSDB Annual Meeting 2019

 



List by Dey Lab

MitoList

This list of preprints is focused on work expanding our knowledge on mitochondria in any organism, tissue or cell type, from the normal biology to the pathology.

 



List by Sandra Franco Iborra

ASCB/EMBO Annual Meeting 2018

This list relates to preprints that were discussed at the recent ASCB conference.

 



List by Dey Lab, Amanda Haage
Close