Close

November in preprints – the CellBio edition

This is the first community-driven preList! A group of preLighters, with expertise in different areas of cell biology, have worked together to create this preprint reading lists for researchers with an interest in cell biology. Categories include: 1) cancer cell biology 2) cell cycle and division 3) cell migration and cytoskeleton 4) cell organelles and organisation 5) cell signalling and mechanosensing 6) genetics/gene expression

List by Felipe Del Valle Batalla, Matthew Davies, Sristilekha Nath, Barbora Knotkova, Vibha SINGH, Girish Kale

Preprints:

Cancer cell biology

DNA repair and anti-cancer mechanisms in the long-lived bowhead whale

Denis Firsanov, Max Zacher, Xiao Tian, Todd L. Sformo, Yang Zhao, Greg Tombline, J. Yuyang Lu, Zhizhong Zheng, Luigi Perelli, Enrico Gurreri, Li Zhang, Jing Guo, Anatoly Korotkov, Valentin Volobaev, Seyed Ali Biashad, Zhihui Zhang, Johanna Heid, Alex Maslov, Shixiang Sun, Zhuoer Wu, Jonathan Gigas, Eric Hillpot, John Martinez, Minseon Lee, Alyssa Williams, Abbey Gilman, Nicholas Hamilton, Ena Haseljic, Avnee Patel, Maggie Straight, Nalani Miller, Julia Ablaeva, Lok Ming Tam, Chloé Couderc, Michael Hoopman, Robert Moritz, Shingo Fujii, Dan J. Hayman, Hongrui Liu, Yuxuan Cai, Anthony K. L. Leung, Mirre J. P. Simons, Zhengdong Zhang, C. Bradley Nelson, Lisa M. Abegglen, Joshua D. Schiffman, Vadim N. Gladyshev, Mauro Modesti, Giannicola Genovese, Jan Vijg, Andrei Seluanov, Vera Gorbunova

https://www.biorxiv.org/content/10.1101/2023.05.07.539748v3

Great preprint that addresses why the very large and long-lived bowhead whale is not so prone to cancer, particularly focussing on DNA repair. It’s relevant because the research question makes total sense and is important but is technically very difficult to address. Here, the authors take on that challenge and thoroughly answer it with a battery of experimental techniques across multiple organisms. Excellent work.

Picked by: Matthew Davies

From Firsanov et al. (2024). This image is made available under a CC-BY-NC-ND 4.0 International license.

Identification of the MRTFA/SRF pathway as a critical regulator of quiescence in cancer

Santiago Panesso-Gómez, Alexander J. Cole, Alyssa Wield, Vivian I. Anyaeche, Jaynish Shah, Qi Jiang, Tonge Ebai, Allison C. Sharrow, George Tseng, Euisik Yoon, Daniel D. Brown, Amanda M. Clark, Scott D Larsen, Ian Eder, David Gau, Partha Roy, Kris N. Dahl, Lam Tran, Hui Jiang, Priscilla F McAuliffe, Adrian V Lee, Ronald J. Buckanovich

https://www.biorxiv.org/content/10.1101/2024.11.15.623825v1

This is an interesting study. Usually cancer stem cells enter quiescence to escape targeting by anti-proliferative chemotherapeutic drugs, re-entering the cell cycle once drug treatment has ended. In this preprint, the authors show that induction and persistence of quiescence by disrupting the MRTF-SRF pathway has therapeutic benefits across a range of cancer lines by preventing re-proliferation.

Picked by: Matthew Davies

From Panesso-Gómez et al. (2024). This image is made available under a CC-BY-NC-ND 4.0 International license.

Cell cycle and division

Tissue-Like Multicellular Development Triggered by Mechanical Compression in Archaea

Theopi Rados, Olivia S. Leland, Pedro Escudeiro, John Mallon, Katherine Andre, Ido Caspy, Andriko von Kügelgen, Elad Stolovicki, Sinead Nguyen, Inés Lucía Patop, Thiberio Rangel, Sebastian Kadener, Lars D. Renner, Vera Thiel, Yoav Soen, Tanmay A.M. Bharat, Vikram Alva, Alex Bisson

https://www.biorxiv.org/content/10.1101/2024.10.19.619234v2

This is a fantastic preprint on the induction of multicellularity in certain Archaea upon compression, cellularising and developing multiple cell types. It demonstrates tissue-like formation from a unicellular organism in real time.

Picked by: Matthew Davies

From Rados et al. (2024). This image is made available under a CC-BY-NC 4.0 International license.

Membrane oscillations driven by Arp2/3 constrict the intercellular bridge during neural stem cell divisions

Bryce LaFoya, Kenneth E. Prehoda

https://doi.org/10.1101/2024.10.28.620743

This preprint is interesting as it addresses a fundamental question revolving around cytokinesis during cell division by emphasising how cells, which are part of the tissue and are constrained, complete the cell division process. The authors found a unique phenomenon involving dynamic membrane oscillation mediated by Arp2/3 actin networks.

Picked by: Sristilekha Nath

From LaFoya and Prehoda (2024). This image is made available under a CC-BY-NC-ND 4.0 International license.

Cell migration and cytoskeleton

Leukocytes use endothelial membrane tunnels to extravasate the vasculature

Werner J. van der Meer, Abraham C.I. van Steen, Eike Mahlandt, Loïc Rolas, Haitao Wang, Janine J.G. Arts, Lanette Kempers, Max L.B. Grönloh, Rianne M. Schoon, Amber Driessen, Jos van Rijssel, Ingeborg Klaassen, Reinier O. Schlingemann, Yosif Manavski, Mark Hoogenboezem, Reinier A. Boon, Satya Khuon, Eric Wait, John Heddleston, Teng-Leong Chew, Martijn A. Nolte, Sussan Nourshargh, Joachim Goedhart, Jaap D. van Buul

https://www.biorxiv.org/content/10.1101/2024.10.28.620560v1

This study reveals a novel mechanism of leukocyte transendothelial migration (TEM), where endothelial cells form membrane tunnels to facilitate leukocyte passage.

Picked by: Felipe Del Valle Batalla

YAP controls cell migration and invasion through a Rho-GTPase switch

Sagar R. Shah, Chunxiao Ren, Nathaniel D. Tippens, JinSeok Park, Ahmed Mohyeldin, Shuyan Wang, Guillermo Vela, Juan C. Martinez-Gutierrez, Seth S. Margolis, Susanne Schmidt, Shuli Xia, Alfredo Quiñones-Hinojosa, Andre Levchenko

https://www.biorxiv.org/content/10.1101/602052v3

This preprint identifies YAP-TRIO regulatory invasion and migration pathway, which reveal potential strategies to target invasive cancers such as glioblastoma.

Picked by: Vibha Singh

From Shah et al. (2024). This image is made available under a CC-BY 4.0 International license.

Fishnet mesh of centrin-Sfi1 drives ultrafast calcium-activated contraction of the giant cell Spirostomum ambiguum

Joseph Lannan, Carlos Floyd, L. X. Xu, Connie Yan, Wallace F. Marshall, Surirayanarayanan Vaikuntanathan, Aaron R. Dinner, Jerry E. Honts, Saad Bhamla, Mary Williard Elting

https://www.biorxiv.org/content/10.1101/2024.11.07.622534v2

Interesting preprint on how a 1 mm-sized unicellular organism contracts to a quarter of its length in about 5 ms and doesn’t use actomyosin contractility to do that.

Picked by: Girish Kale

From Lannan et al. (2024). This image is made available under a CC-BY-NC-ND 4.0 International license.

Cell organelles and organisation

Small molecule modulator of neuronal lysosome positioning and function resolves Alzheimer’s Disease-linked pathologies in cultured human neurons

Amanda M. Snead, Sruchi Patel, Mia Krout, Ryan S. Hippman, Gabrielle Smith, Diya Dileep, Nitya Chagoor, Rachel Shi, Ricardo Linares, Andrew Dobria, Stephanie M. Cologna, Camerron Crowder, Leslie N. Aldrich, Swetha Gowrishankar

https://www.biorxiv.org/content/10.1101/2024.11.04.621986v1

The authors describe a small molecule that selectively regulates lysosome positioning in neurons. This molecule ameliorates neurodegeneration and Alzheimer’s features.

Picked by: Felipe Del Valle Batalla

From Snead et al. (2024). This image is made available under a CC-BY-NC-ND 4.0 International license.

Alternative Import-Channels And Destinations Of Mitochondrial PINK1 Controlled By Trans-Membrane-Domain Structural Plasticity

James S. Lorriman, Adam G. Grieve, Robin A. Corey, Ian Collinson

https://doi.org/10.1101/2024.11.06.622366

I think that this is a great preprint that provides fantastic mechanistic details of PINK kinase’s import into mitochondria. It solidifies a direct role of PINK within the matrix of healthy mitochondria and opens up interesting new avenues for targeting PINK in Parkinson’s.

Picked by: Barbora Knotkova

From Lorriman et al. (2024). This image is made available under a CC-BY 4.0 International license.

Vimentin molecular linkages with nesprin-3 enhance nuclear deformations by cell geometric constraints

Maxx Swoger, Minh Tri Ho Thanh, Fitzroy J. Byfield, Van Dam, Jessica Williamson, Bronson Frank, Heidi Hehnly, Daniel Conway, Alison E. Patteson

https://www.biorxiv.org/content/10.1101/2024.10.29.621001v1

The study reveals the role of vimentin (an intermediate filament) in mediating nuclear shape deformation, which, in turn, promotes chromatin condensation under conditions of geometric constraint.

Picked by: Sristilekha Nath

Spectrin coordinates cortical actomyosin organization and differentiation essential for a functional epithelial barrier

Arad Soffer, Aishwarya Bhosale, Takeshi Matsui, Carien M. Niessen, Chen Luxenburg, Matthias Rübsam

https://www.biorxiv.org/content/10.1101/2024.10.17.618796v2.full

This study uncovers the role of cell shape in regulating cell differentiation, which is mediated by cortically recruited Spectrin, thereby promoting barrier functions in the epidermis.

Picked by: Sristilekha Nath

From Soffer et al. (2024). This image is made available under a CC-BY-NC-ND 4.0 International license.

Automated segmentation of soft X-ray tomography: native cellular structure with sub-micron resolution at high throughput for whole-cell quantitative imaging in yeast

Jianhua Chen, Mary Mirvis, Axel Ekman, Bieke Vanslembrouck, Mark Le Gros, Carolyn Larabell, Wallace F. Marshall

https://www.biorxiv.org/content/10.1101/2024.10.31.621371v1

This preprint is interesting because it uses soft X-ray tomography and deep learning tools for exploring whole-cells and imaging of multi-organelle 3D volumes. This can help with analysis of cell and organelle morphology, interactions, and responses to perturbations.

Picked by: Felipe Del Valle Batalla

Adipose-derived Mesenchymal Stem Cells and Retinal Pigment Epithelial Cells Interactions in Stress Environment via Tunneling Nanotubes

Merve Gozel, Karya Senkoylu, Cem Kesim, Murat Hasanreisoglu

https://www.biorxiv.org/content/10.1101/2024.11.24.624852v1

This is a cool preprint describing the transfer of mitochondria from healthy mesenchymal stem cells to stressed retinal pigment epithelial cells through tunelling nanotubes, allowing recovery. It has implications for potential stem cell-based therapies for retinal disease.

Picked by: Matthew Davies

From Gozel et al. (2024). This image is made available under a CC-BY-NC-ND 4.0 International license.

Compositional Flexibility of the ER-Mitochondria Encounter Structure

Christian Covill-Cooke, Takashi Hirashima, Shin Kawano, Joe Ganellin, Andrew Moody, Sabine N.S. van Schie, Arun T. John Peter, Chika Saito, Toshiya Endo, Benoît Kornmann

https://doi.org/10.1101/2024.11.26.625358

Preprint that identifies the minimal subunits of the ERMES complex required for lipid transfer between the ER and mitochondria in yeast. It solves the mystery of how the expression of artificial tethers can restore lipid transfer in the absence of other ERMES subunits.

Picked by: Barbora Knotkova

From Covill-Cooke et al. (2024). This image is made available under a CC-BY 4.0 International license.

The Rho effector ARHGAP18 coordinates a Hippo pathway feedback loop through YAP and Merlin to regulate the cytoskeleton and epithelial cell polarity

Emma C. Murray, Gilian M. Hodge, Leighton S. Lee, Cameron A.R. Mitchell, Andrew T. Lombardo

https://doi.org/10.1101/2024.11.26.625473

In this preprint, the authors reveal how Hippo and Rho family signaling pathways synergistically regulate actin cytoskeleton organization, bridging the previously assumed non-coordination between them.

Picked by: Vibha Singh

From Murray et al. (2024). This image is made available under a CC-BY-NC 4.0 International license.

Cell signalling and mechanosensing

Live-cell magnetic micromanipulation of recycling endosomes reveals their direct effect on actin-based protrusions to promote invasive migration

Jakub Gemperle, Domenik Liße, Marie Kappen, Emilie Secret, Mathieu Coppey, Martin Gregor, Christine Menager, Jacob Piehler, Patrick Caswell

https://www.biorxiv.org/content/10.1101/2024.11.11.622870v1

This preprint is amazing! Why: the authors show manipulation of vesicles via magnetogenetics.

Picked by: Felipe Del Valle Batalla

From Gemperle et al. (2024). This image is made available under a CC-BY 4.0 International license.

Semaphorin 4A maintains functional diversity of the hematopoietic stem cell pool

Dorsa Toghani, Sanika Gupte, Sharon Zeng, Elmir Mahammadov, Edie I. Crosse, Negar Seyedhassantehrani, Christian Burns, David Gravano, Stefan Radtke, Hans-Peter Kiem, Sonia Rodriguez, Nadia Carlesso, Amogh Pradeep, Alexis Georgiades, Fabienne Lucas, Nicola K. Wilson, Sarah J. Kinston, Berthold Göttgens, Le Zong, Isabel Beerman, Bongsoo Park, Derek Janssens, Daniel Jones, Ali Toghani, Claus Nerlov, Eric Pietras, Marion Mesnieres, Christa Maes, Atsushi Kumanogoh, Thomas Worzfeld, Jin-Gyu Cheong, Steven Z Josefowicz, Peter Kharchenko, David T. Scadden, Antonio Scialdone, Joel A Spencer, Lev Silberstein

https://www.biorxiv.org/content/10.1101/2024.11.12.622506v1

This is a nice preprint. It concerns non-cell autonomous regulation of haematopoeitic stem cells with implications for therapeutic targeting of HSC decline with ageing.

Picked by: Matthew Davies

Membrane potential mediates the cellular response to mechanical pressure

Avik Mukherjee, Yanqing Huang, Jens Elgeti, Seungeun Oh, Jose G. Abreu, Anjali Rebecca Neliat, Janik Schüttler, Dan-Dan Su, Christophe Dupre, Nina Catherine Benites, Xili Liu, Leonid Peshkin, Mihail Barboiu, Hugo Stocker, Marc W. Kirschner, Markus Basan

https://doi.org/10.1101/2023.11.02.565386

This preprint identifies membrane potential as the missing link between mechanical force and downstream cellular responses.

Picked by: Barbora Knotkova

From Mukherjee et al. (2024). This image is made available under a CC-BY-NC-ND 4.0 International license.

Compressive forces induce epigenetic activation of aged human dermal fibroblasts through ERK signaling pathway

Hui Liu, Luezhen Yuan, Lucrezia Baldi, Trinadha Rao Sornapudi, G.V. Shivashankar

https://doi.org/10.1101/2024.11.04.621794

This preprint reports activation of cellular rejuvenation pathways via epigenetic modifications, when aged dermal fibroblasts were subjected to sustained compressive forces.

Picked by: Sristilekha Nath

From Liu et al. (2024). This image is made available under a CC-BY-NC-ND 4.0 International license.

Microglia States are Susceptible to Senescence and Cholesterol Dysregulation in Alzheimer’s Disease

Boyang Li, Shaowei Wang, Bilal Kerman, Cristelle Hugo, E Keats Shwab, Chang Shu, Ornit Chiba-Falek, Zoe Arvanitakis, Hussein Yassine

https://www.biorxiv.org/content/10.1101/2024.11.18.624141v1

This study is important because it identifies cholesterol dysregulation as a key driver of microglial senescence in Alzheimer’s disease, offering potential new therapeutic targets.

Picked by: Felipe Del Valle Batalla

Piezo1 balances membrane tension and cortical contractility to stabilize intercellular junctions and maintain epithelial barrier integrity

Ahsan Javed, Aki Stubb, Clémentine Villeneuve, Franziska Peters, Matthias Rübsam, Carien M. Niessen, Leah C. Biggs, Sara A. Wickström

https://www.biorxiv.org/content/10.1101/2024.11.20.624378v1

Preprint describing the role of mechanosensitive ion channel Piezo1 in the mechanically tensed epidermis, where it regulates Tight-junctions and epithelial barrier function.

Picked by: Girish Kale

From Javed et al. (2024). This image is made available under a CC-BY-NC 4.0 International license.

Genetics/gene expression

The 3’-untranslated regions of yeast ribosomal protein mRNAs determine paralog incorporation into ribosomes and recruit factors necessary for specialized functions

Raman K. Singh, Robert A. Crawford, Dheerendra P. Mall, Graham D. Pavitt, Jeffrey E. Gerst

https://doi.org/10.1101/2024.03.18.585503

The study represents the first comprehensive analysis of the role of ribosome heterogeneity in yeast under different growth conditions.

Picked by: Barbora Knotkova

From Singh al. (2024). This image is made available under a CC-BY-NC-ND 4.0 International license.

Have your say

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

Sign up to customise the site to your preferences and to receive alerts

Register here

Also in the cell biology category:

BSCB-Biochemical Society 2024 Cell Migration meeting

This preList features preprints that were discussed and presented during the BSCB-Biochemical Society 2024 Cell Migration meeting in Birmingham, UK in April 2024. Kindly put together by Sara Morais da Silva, Reviews Editor at Journal of Cell Science.

 



List by Reinier Prosee

‘In preprints’ from Development 2022-2023

A list of the preprints featured in Development's 'In preprints' articles between 2022-2023

 



List by Alex Eve, Katherine Brown

preLights peer support – preprints of interest

This is a preprint repository to organise the preprints and preLights covered through the 'preLights peer support' initiative.

 



List by preLights peer support

The Society for Developmental Biology 82nd Annual Meeting

This preList is made up of the preprints discussed during the Society for Developmental Biology 82nd Annual Meeting that took place in Chicago in July 2023.

 



List by Joyce Yu, Katherine Brown

CSHL 87th Symposium: Stem Cells

Preprints mentioned by speakers at the #CSHLsymp23

 



List by Alex Eve

Journal of Cell Science meeting ‘Imaging Cell Dynamics’

This preList highlights the preprints discussed at the JCS meeting 'Imaging Cell Dynamics'. The meeting was held from 14 - 17 May 2023 in Lisbon, Portugal and was organised by Erika Holzbaur, Jennifer Lippincott-Schwartz, Rob Parton and Michael Way.

 



List by Helen Zenner

9th International Symposium on the Biology of Vertebrate Sex Determination

This preList contains preprints discussed during the 9th International Symposium on the Biology of Vertebrate Sex Determination. This conference was held in Kona, Hawaii from April 17th to 21st 2023.

 



List by Martin Estermann

Alumni picks – preLights 5th Birthday

This preList contains preprints that were picked and highlighted by preLights Alumni - an initiative that was set up to mark preLights 5th birthday. More entries will follow throughout February and March 2023.

 



List by Sergio Menchero et al.

CellBio 2022 – An ASCB/EMBO Meeting

This preLists features preprints that were discussed and presented during the CellBio 2022 meeting in Washington, DC in December 2022.

 



List by Nadja Hümpfer et al.

Fibroblasts

The advances in fibroblast biology preList explores the recent discoveries and preprints of the fibroblast world. Get ready to immerse yourself with this list created for fibroblasts aficionados and lovers, and beyond. Here, my goal is to include preprints of fibroblast biology, heterogeneity, fate, extracellular matrix, behavior, topography, single-cell atlases, spatial transcriptomics, and their matrix!

 



List by Osvaldo Contreras

EMBL Synthetic Morphogenesis: From Gene Circuits to Tissue Architecture (2021)

A list of preprints mentioned at the #EESmorphoG virtual meeting in 2021.

 



List by Alex Eve

FENS 2020

A collection of preprints presented during the virtual meeting of the Federation of European Neuroscience Societies (FENS) in 2020

 



List by Ana Dorrego-Rivas

Planar Cell Polarity – PCP

This preList contains preprints about the latest findings on Planar Cell Polarity (PCP) in various model organisms at the molecular, cellular and tissue levels.

 



List by Ana Dorrego-Rivas

BioMalPar XVI: Biology and Pathology of the Malaria Parasite

[under construction] Preprints presented at the (fully virtual) EMBL BioMalPar XVI, 17-18 May 2020 #emblmalaria

 



List by Dey Lab, Samantha Seah

1

Cell Polarity

Recent research from the field of cell polarity is summarized in this list of preprints. It comprises of studies focusing on various forms of cell polarity ranging from epithelial polarity, planar cell polarity to front-to-rear polarity.

 



List by Yamini Ravichandran

TAGC 2020

Preprints recently presented at the virtual Allied Genetics Conference, April 22-26, 2020. #TAGC20

 



List by Maiko Kitaoka et al.

3D Gastruloids

A curated list of preprints related to Gastruloids (in vitro models of early development obtained by 3D aggregation of embryonic cells). Updated until July 2021.

 



List by Paul Gerald L. Sanchez and Stefano Vianello

ECFG15 – Fungal biology

Preprints presented at 15th European Conference on Fungal Genetics 17-20 February 2020 Rome

 



List by Hiral Shah

ASCB EMBO Annual Meeting 2019

A collection of preprints presented at the 2019 ASCB EMBO Meeting in Washington, DC (December 7-11)

 



List by Madhuja Samaddar et al.

EMBL Seeing is Believing – Imaging the Molecular Processes of Life

Preprints discussed at the 2019 edition of Seeing is Believing, at EMBL Heidelberg from the 9th-12th October 2019

 



List by Dey Lab

Autophagy

Preprints on autophagy and lysosomal degradation and its role in neurodegeneration and disease. Includes molecular mechanisms, upstream signalling and regulation as well as studies on pharmaceutical interventions to upregulate the process.

 



List by Sandra Malmgren Hill

Lung Disease and Regeneration

This preprint list compiles highlights from the field of lung biology.

 



List by Rob Hynds

Cellular metabolism

A curated list of preprints related to cellular metabolism at Biorxiv by Pablo Ranea Robles from the Prelights community. Special interest on lipid metabolism, peroxisomes and mitochondria.

 



List by Pablo Ranea Robles

BSCB/BSDB Annual Meeting 2019

Preprints presented at the BSCB/BSDB Annual Meeting 2019

 



List by Dey Lab

MitoList

This list of preprints is focused on work expanding our knowledge on mitochondria in any organism, tissue or cell type, from the normal biology to the pathology.

 



List by Sandra Franco Iborra

Biophysical Society Annual Meeting 2019

Few of the preprints that were discussed in the recent BPS annual meeting at Baltimore, USA

 



List by Joseph Jose Thottacherry

ASCB/EMBO Annual Meeting 2018

This list relates to preprints that were discussed at the recent ASCB conference.

 



List by Dey Lab, Amanda Haage

Also in the cell biology category:

Motor Clustering Enhances Kinesin-driven Vesicle Transport

Rui Jiang, Qingzhou Feng, Daguan Nong, et al.

Selected by 16 November 2024

Sharvari Pitke

Biophysics

Cellular signalling protrusions enable dynamic distant contacts in spinal cord neurogenesis

Joshua Hawley, Robert Lea, Veronica Biga, et al.

Selected by 15 November 2024

Ankita Walvekar

Developmental Biology

Green synthesized silver nanoparticles from Moringa: Potential for preventative treatment of SARS-CoV-2 contaminated water

Adebayo J. Bello, Omorilewa B. Ebunoluwa, Rukayat O. Ayorinde, et al.

Selected by 14 November 2024

Safieh Shah, Benjamin Dominik Maier

Epidemiology
Close