mTORC1 is required for differentiation of germline stem cells in the Drosophila melanogaster testis
Posted on: 28 November 2022 , updated on: 8 May 2024
Preprint posted on 25 July 2022
Article now published in PLOS ONE at http://dx.doi.org/10.1371/journal.pone.0300337
One signal to differentiate them both and in timed development bind them: Tor is required for germ cell differentiation and its coordination with supporting somatic cells in the Drosophila testis
Selected by Diego Sainz de la Maza
Categories: cell biology, developmental biology, genetics
Updated 8 May 2024 with a postLight by Diego Sainz de la Maza
The highlighted preprint has recently been published in PLOS ONE.
The core of the work remains quite similar to what was presented in the preprint, but further quantifications provided by the authors make their conclusions more solid.
One of the main points brought forward by this work is that Tor is involved in germ cell differentiation. The authors knocked down Tor in the germline and observed an increase in germ cells with dotted-like structures called spectrosomes, a hallmark of their undifferentiated status. In the peer-reviewed paper, they quantified this observation and confirmed their results. They also validated this phenotype by using several RNAi constructs and by checking that Tor RNAi drove the expected decrease in the levels of pS6. Intriguingly, the quantifications show that even when Tor knockdown causes early germ cells to accumulate, the number of germline stem cells contacting the niche decreases.
In their preprint, the authors reported large cytoplasmic structures in Tor-depleted early germ cells which were absent in controls. They stained for Lysotracker and Atg8a and claimed that these structures were large autophagosomes. In the peer-reviewed paper, they have quantified the number of autophagosomes and their size, confirming their previous observations.
Overall, the authors have further supported the conclusions of the preprint with stronger data, mainly quantifications for their observed phenotypes.
Investigating how Tor regulates stem cell numbers and how Tor coordinates the differentiation of both germ and somatic lineages remain interesting future directions to study.
Background
Adult stem cells maintain tissue homeostasis by balancing self-renewal and differentiation. They reside in specialized microenvironments termed niches, where they receive supporting signaling molecules. Metabolism has been increasingly linked to cell fate decisions, but how metabolic cues are integrated with the signaling occurring in the stem cell niche is still unclear.
The Drosophila testis has a stem cell niche located at the apical tip of the tissue, which suports two different stem cell populations. Germline stem cells (GSCs) self-renew and give rise to gonialblasts, which undergo four uncomplete divisions and produce germ cysts consisting of 2, 4, 8 or 16 spermatogonia. Somatic stem cells are termed cyst stem cells, and give rise to cyst cells, which ensheath the germline and support it throughout its development.
Previous findings suggest that the testis is highly sensitive to metabolic perturbations. Firstly, citrate from the gut reaches the testis and is required for sperm maturation. Secondly, protein starvation causes the death of transit-amplifying germ cells. In addition, insulin signaling is required by supporting somatic cells to differentiate. And finally, previous work from the Jones Lab showed that depletion of the regulator of mitochondrial fusion Mitofusin induced loss of GSCs in a TOR-dependent manner.
The preprint I have chosen to highlight explores the effects of TOR signaling on the germline and the entire tissue in great detail.
Why I chose this study
Metabolism has often been regarded as a mere succession of chemical reactions aimed to either obtain energy or synthesize macromolecules. From that point of view, metabolism would be a downstream effector of cellular choices already taken at the transcriptional level.
However, it has been reported that increasing activity of specific metabolic pathways can affect cell proliferative capacity or influence cell fate choices. These observations raise the possibility that metabolism can also influence cellular decision making. Considering that cells reside in tissues and are continuously sensing their surrounding environment, it seems reasonable that nutrient availability and the subsequent activity of metabolic pathways has certain degree of crosstalk with signaling pathways and ultimately with cell behaviour.
As far as stem cells are concerned, they are involved in tremendously complex processes such as development or tissue homeostasis. One could predict that stem cells need to coordinate the production of new cells to a favourable nutritional or metabolic status, a very exciting field that has not been extensively explored.
This highlighted preprint explores the role of TOR in germline differentiation. I chose this preprint because previous work had already linked TOR activity as a requirement for differentiation of somatic cells. Do both the soma and the germline integrate metabolic cues through TOR signaling? Is TOR responsible for differentiation in both cases? In addition to the effects that TOR could have on both lineages autonomously, this preprint raises another important question, which is how the cell identity of a cell affects other surrounding cell types when they must differentiate in a coordinated manner, as can be observed in the testis.
Main findings
TOR is present and active in early germ cells
The authors studied the presence and activity of TOR across the germline. To study its location, they used a TOR::GFP fusion protein. They reported that GSCs and early germ cells had higher levels of TOR than differentiating spermatogonia, which had lower levels and were restricted to the nucleus. In order to check where TOR is active, they used an antibody against the phosphorylated form of a TOR target and detected its signal in a subset of GSCs and early germ cells. Thus, TOR is present mostly in early germ cells and is active in a subset of them. When germ cells differentiate, TOR levels decrease.
TOR activity is required in early germ cells for differentiation
The authors addressed the impact of TOR on germ cell identity by performing loss of function experiments. They knocked down TOR in the germline and observed an accumulation of early germ cells in the tissue. No phenotype was observed when TOR was knocked down in germ cells that had started their differentiation program. This suggests that TOR activity is required in early germ cells for their differentiation and once its differentiation program has started TOR is downregulated and no longer required.
TOR knockdown in early germ cells triggers differentiation of somatic cells
Finally, the authors asked if disruption of germ cell identity had a non-autonomous effect on the neighbouring somatic cells. They knocked down TOR in the germline and studied somatic cell identity. They observed that TOR knockdown in the germline significantly decreased the number of somatic cells expressing a marker for early somatic cells. They checked if somatic cells were lost to differentiation in this context and found that knockdown of TOR in the germline upregulated TOR activity in the soma and caused the premature appearance of differentiation markers in the somatic cells. These data suggests that TOR downregulation couples germ cell and soma differentiation.
In conclusion, these findings support a model where active TOR in the germline is required to start its differentiation program and once this is triggered TOR signaling is no longer required. This downregulation of TOR in the germline is sensed by the soma, which starts its differentiation program to support the germline.
doi: https://doi.org/10.1242/prelights.33199
Read preprint
(No Ratings Yet)Discussion with the authors
Marie Clémot shared
Question 1. The authors find an interesting heterogeneous pattern of TOR activity across the germline. Can the authors speculate what could be the mechanism behind it? Could it be differential levels of signals arriving from the niche? Could this be related to the reported production of inhibitors of insulin signaling by the surrounding somatic stem cells?
Answer. That is an interesting thought. I think that we favour a different hypothesis. A similar patchy pattern of Tor activity was previously reported in developing wing discs. We think that pS6, the TOR target that we used as a readout for TOR activity, is cell cycle dependent and the heterogenous positivity reflects the proliferation status. We cannot rule out the effect of a signal from the niche sensed differently by the germ cells, but it is unlikely that this is due to the secretion of insulin inhibitors by somatic stem cells because that would only affect the germ cells adjacent to the niche and our pS6 staining labels several stages of the germ cell lineage, including GSCs and germ cells more distant from the hub.
Question 2. The loss of function experiments show that TOR downregulation disrupts differentiation of the germline and does not affect differentiation of the germ cells which have already started differentiating. This result suggests that active TOR is required at the onset of differentiation and then is progressively downregulated. It could be interesting to perform loss of function experiments that drive hyperactivation of the TOR pathway, such as knockdown of Tsc1, to better understand the requirement of TOR in the differentiation of the germline. In this context, is it possible that a block of differentiation would still take place since there would not be a downregulation in differentiating germ cells? Alternatively, could it also drive premature differentiation of the germline?
Answer. We think that hyperactivated TOR would cause premature differentiation of the germline. In a previous paper from the lab, we observed that increased TOR activity in early germ cells with perturbed mitochondrial function (knockdown of the mitochondrial fusion regulator mitofusin) was associated with a loss of GSCs, which could be rescued if TOR was inhibited. When we knockdown TSC1 or TSC2 in GSCs and early germ cells, we get the expected increase in active TOR, but the phenotype in the tissue is not striking. These data suggest that hyperactivated TOR would cause premature differentiation of the germline, but it could be difficult to see because TOR seems to be required on the onset of differentiation and then is no longer required. I tried to hyperactivate TOR in differentiating germ cells using a different driver but did not observe a phenotype, probably because this driver turns on too late, at a stage where the Tor kinase is no longer highly expressed.
Question 3. The authors show that TOR is involved in the proper differentiation timing of both the germline and the soma. Upon TOR downregulation in the germline, somatic cells express a differentiation marker prematurely. The authors speculate that TOR could be inhibiting the secretion of Spi from the germline to the soma, a reported differentiation signal for somatic cells. It could be relatively easy to test this hypothesis by using in situ hybridization in the tissue and either quantify or locate the expression of Spi both in control tissue and when TOR is downregulated in the germline. Are there any plans to do anything along these lines?
Answer. We focused on the possible impairment of Spi secretion at the protein level. In the preprint, we include data showing that knocking down TOR in the germline causes an accumulation of dysfunctional autolysosomes in early germ cells. We thought that there could be a defect in the germline to secret Spi and activate differentiation in somatic cells. Unfortunately, we could not find a good antibody or reporter. We didn’t study Spi at the transcriptional level, but it could be interesting to do so.
Question 4. Is there anything else that you would like to comment on?
Answer. I think that the preprint provides relevant data about the coordination of the differentiation of both soma and germline. I would like to follow up on that. But I was also very interested on the acumulation of autolysosomes when TOR is knocked down. They have a very big size, occupying part of the cytoplasm of the germ cells.
This is intriguing because when we knocked down genes involved in autophagy, we did not see a phenotype. Then, why would autophagy become dysfunctional when TOR is knocked down if does not seem to be required for normal differentiation?
Sign up to customise the site to your preferences and to receive alerts
Register hereAlso in the cell biology category:
Motor Clustering Enhances Kinesin-driven Vesicle Transport
Sharvari Pitke
Cellular signalling protrusions enable dynamic distant contacts in spinal cord neurogenesis
Ankita Walvekar
Green synthesized silver nanoparticles from Moringa: Potential for preventative treatment of SARS-CoV-2 contaminated water
Safieh Shah, Benjamin Dominik Maier
Also in the developmental biology category:
Cellular signalling protrusions enable dynamic distant contacts in spinal cord neurogenesis
Ankita Walvekar
Actin-based deformations of the nucleus control multiciliated ependymal cell differentiation
Ryan Harrison
HIF1A contributes to the survival of aneuploid and mosaic pre-implantation embryos
Anchel De Jaime Soguero
Also in the genetics category:
Intracellular diffusion in the cytoplasm increases with cell size in fission yeast
Leeba Ann Chacko, Sameer Thukral
Significantly reduced, but balanced, rates of mitochondrial fission and fusion are sufficient to maintain the integrity of yeast mitochondrial DNA
Leeba Ann Chacko
The bat Influenza A virus subtype H18N11 induces nanoscale MHCII clustering upon host cell attachment
Mitchell Sarmie, Mohammed A. Jalloh
preLists in the cell biology category:
BSCB-Biochemical Society 2024 Cell Migration meeting
This preList features preprints that were discussed and presented during the BSCB-Biochemical Society 2024 Cell Migration meeting in Birmingham, UK in April 2024. Kindly put together by Sara Morais da Silva, Reviews Editor at Journal of Cell Science.
| List by | Reinier Prosee |
‘In preprints’ from Development 2022-2023
A list of the preprints featured in Development's 'In preprints' articles between 2022-2023
| List by | Alex Eve, Katherine Brown |
preLights peer support – preprints of interest
This is a preprint repository to organise the preprints and preLights covered through the 'preLights peer support' initiative.
| List by | preLights peer support |
The Society for Developmental Biology 82nd Annual Meeting
This preList is made up of the preprints discussed during the Society for Developmental Biology 82nd Annual Meeting that took place in Chicago in July 2023.
| List by | Joyce Yu, Katherine Brown |
CSHL 87th Symposium: Stem Cells
Preprints mentioned by speakers at the #CSHLsymp23
| List by | Alex Eve |
Journal of Cell Science meeting ‘Imaging Cell Dynamics’
This preList highlights the preprints discussed at the JCS meeting 'Imaging Cell Dynamics'. The meeting was held from 14 - 17 May 2023 in Lisbon, Portugal and was organised by Erika Holzbaur, Jennifer Lippincott-Schwartz, Rob Parton and Michael Way.
| List by | Helen Zenner |
9th International Symposium on the Biology of Vertebrate Sex Determination
This preList contains preprints discussed during the 9th International Symposium on the Biology of Vertebrate Sex Determination. This conference was held in Kona, Hawaii from April 17th to 21st 2023.
| List by | Martin Estermann |
Alumni picks – preLights 5th Birthday
This preList contains preprints that were picked and highlighted by preLights Alumni - an initiative that was set up to mark preLights 5th birthday. More entries will follow throughout February and March 2023.
| List by | Sergio Menchero et al. |
CellBio 2022 – An ASCB/EMBO Meeting
This preLists features preprints that were discussed and presented during the CellBio 2022 meeting in Washington, DC in December 2022.
| List by | Nadja Hümpfer et al. |
Fibroblasts
The advances in fibroblast biology preList explores the recent discoveries and preprints of the fibroblast world. Get ready to immerse yourself with this list created for fibroblasts aficionados and lovers, and beyond. Here, my goal is to include preprints of fibroblast biology, heterogeneity, fate, extracellular matrix, behavior, topography, single-cell atlases, spatial transcriptomics, and their matrix!
| List by | Osvaldo Contreras |
EMBL Synthetic Morphogenesis: From Gene Circuits to Tissue Architecture (2021)
A list of preprints mentioned at the #EESmorphoG virtual meeting in 2021.
| List by | Alex Eve |
FENS 2020
A collection of preprints presented during the virtual meeting of the Federation of European Neuroscience Societies (FENS) in 2020
| List by | Ana Dorrego-Rivas |
Planar Cell Polarity – PCP
This preList contains preprints about the latest findings on Planar Cell Polarity (PCP) in various model organisms at the molecular, cellular and tissue levels.
| List by | Ana Dorrego-Rivas |
BioMalPar XVI: Biology and Pathology of the Malaria Parasite
[under construction] Preprints presented at the (fully virtual) EMBL BioMalPar XVI, 17-18 May 2020 #emblmalaria
| List by | Dey Lab, Samantha Seah |
1
Cell Polarity
Recent research from the field of cell polarity is summarized in this list of preprints. It comprises of studies focusing on various forms of cell polarity ranging from epithelial polarity, planar cell polarity to front-to-rear polarity.
| List by | Yamini Ravichandran |
TAGC 2020
Preprints recently presented at the virtual Allied Genetics Conference, April 22-26, 2020. #TAGC20
| List by | Maiko Kitaoka et al. |
3D Gastruloids
A curated list of preprints related to Gastruloids (in vitro models of early development obtained by 3D aggregation of embryonic cells). Updated until July 2021.
| List by | Paul Gerald L. Sanchez and Stefano Vianello |
ECFG15 – Fungal biology
Preprints presented at 15th European Conference on Fungal Genetics 17-20 February 2020 Rome
| List by | Hiral Shah |
ASCB EMBO Annual Meeting 2019
A collection of preprints presented at the 2019 ASCB EMBO Meeting in Washington, DC (December 7-11)
| List by | Madhuja Samaddar et al. |
EMBL Seeing is Believing – Imaging the Molecular Processes of Life
Preprints discussed at the 2019 edition of Seeing is Believing, at EMBL Heidelberg from the 9th-12th October 2019
| List by | Dey Lab |
Autophagy
Preprints on autophagy and lysosomal degradation and its role in neurodegeneration and disease. Includes molecular mechanisms, upstream signalling and regulation as well as studies on pharmaceutical interventions to upregulate the process.
| List by | Sandra Malmgren Hill |
Lung Disease and Regeneration
This preprint list compiles highlights from the field of lung biology.
| List by | Rob Hynds |
Cellular metabolism
A curated list of preprints related to cellular metabolism at Biorxiv by Pablo Ranea Robles from the Prelights community. Special interest on lipid metabolism, peroxisomes and mitochondria.
| List by | Pablo Ranea Robles |
BSCB/BSDB Annual Meeting 2019
Preprints presented at the BSCB/BSDB Annual Meeting 2019
| List by | Dey Lab |
MitoList
This list of preprints is focused on work expanding our knowledge on mitochondria in any organism, tissue or cell type, from the normal biology to the pathology.
| List by | Sandra Franco Iborra |
Biophysical Society Annual Meeting 2019
Few of the preprints that were discussed in the recent BPS annual meeting at Baltimore, USA
| List by | Joseph Jose Thottacherry |
ASCB/EMBO Annual Meeting 2018
This list relates to preprints that were discussed at the recent ASCB conference.
| List by | Dey Lab, Amanda Haage |
Also in the developmental biology category:
BSDB/GenSoc Spring Meeting 2024
A list of preprints highlighted at the British Society for Developmental Biology and Genetics Society joint Spring meeting 2024 at Warwick, UK.
| List by | Joyce Yu, Katherine Brown |
GfE/ DSDB meeting 2024
This preList highlights the preprints discussed at the 2024 joint German and Dutch developmental biology societies meeting that took place in March 2024 in Osnabrück, Germany.
| List by | Joyce Yu |
‘In preprints’ from Development 2022-2023
A list of the preprints featured in Development's 'In preprints' articles between 2022-2023
| List by | Alex Eve, Katherine Brown |
preLights peer support – preprints of interest
This is a preprint repository to organise the preprints and preLights covered through the 'preLights peer support' initiative.
| List by | preLights peer support |
The Society for Developmental Biology 82nd Annual Meeting
This preList is made up of the preprints discussed during the Society for Developmental Biology 82nd Annual Meeting that took place in Chicago in July 2023.
| List by | Joyce Yu, Katherine Brown |
CSHL 87th Symposium: Stem Cells
Preprints mentioned by speakers at the #CSHLsymp23
| List by | Alex Eve |
Journal of Cell Science meeting ‘Imaging Cell Dynamics’
This preList highlights the preprints discussed at the JCS meeting 'Imaging Cell Dynamics'. The meeting was held from 14 - 17 May 2023 in Lisbon, Portugal and was organised by Erika Holzbaur, Jennifer Lippincott-Schwartz, Rob Parton and Michael Way.
| List by | Helen Zenner |
9th International Symposium on the Biology of Vertebrate Sex Determination
This preList contains preprints discussed during the 9th International Symposium on the Biology of Vertebrate Sex Determination. This conference was held in Kona, Hawaii from April 17th to 21st 2023.
| List by | Martin Estermann |
Alumni picks – preLights 5th Birthday
This preList contains preprints that were picked and highlighted by preLights Alumni - an initiative that was set up to mark preLights 5th birthday. More entries will follow throughout February and March 2023.
| List by | Sergio Menchero et al. |
CellBio 2022 – An ASCB/EMBO Meeting
This preLists features preprints that were discussed and presented during the CellBio 2022 meeting in Washington, DC in December 2022.
| List by | Nadja Hümpfer et al. |
2nd Conference of the Visegrád Group Society for Developmental Biology
Preprints from the 2nd Conference of the Visegrád Group Society for Developmental Biology (2-5 September, 2021, Szeged, Hungary)
| List by | Nándor Lipták |
Fibroblasts
The advances in fibroblast biology preList explores the recent discoveries and preprints of the fibroblast world. Get ready to immerse yourself with this list created for fibroblasts aficionados and lovers, and beyond. Here, my goal is to include preprints of fibroblast biology, heterogeneity, fate, extracellular matrix, behavior, topography, single-cell atlases, spatial transcriptomics, and their matrix!
| List by | Osvaldo Contreras |
EMBL Synthetic Morphogenesis: From Gene Circuits to Tissue Architecture (2021)
A list of preprints mentioned at the #EESmorphoG virtual meeting in 2021.
| List by | Alex Eve |
EMBL Conference: From functional genomics to systems biology
Preprints presented at the virtual EMBL conference "from functional genomics and systems biology", 16-19 November 2020
| List by | Jesus Victorino |
Single Cell Biology 2020
A list of preprints mentioned at the Wellcome Genome Campus Single Cell Biology 2020 meeting.
| List by | Alex Eve |
Society for Developmental Biology 79th Annual Meeting
Preprints at SDB 2020
| List by | Irepan Salvador-Martinez, Martin Estermann |
FENS 2020
A collection of preprints presented during the virtual meeting of the Federation of European Neuroscience Societies (FENS) in 2020
| List by | Ana Dorrego-Rivas |
Planar Cell Polarity – PCP
This preList contains preprints about the latest findings on Planar Cell Polarity (PCP) in various model organisms at the molecular, cellular and tissue levels.
| List by | Ana Dorrego-Rivas |
Cell Polarity
Recent research from the field of cell polarity is summarized in this list of preprints. It comprises of studies focusing on various forms of cell polarity ranging from epithelial polarity, planar cell polarity to front-to-rear polarity.
| List by | Yamini Ravichandran |
TAGC 2020
Preprints recently presented at the virtual Allied Genetics Conference, April 22-26, 2020. #TAGC20
| List by | Maiko Kitaoka et al. |
3D Gastruloids
A curated list of preprints related to Gastruloids (in vitro models of early development obtained by 3D aggregation of embryonic cells). Updated until July 2021.
| List by | Paul Gerald L. Sanchez and Stefano Vianello |
ASCB EMBO Annual Meeting 2019
A collection of preprints presented at the 2019 ASCB EMBO Meeting in Washington, DC (December 7-11)
| List by | Madhuja Samaddar et al. |
EDBC Alicante 2019
Preprints presented at the European Developmental Biology Congress (EDBC) in Alicante, October 23-26 2019.
| List by | Sergio Menchero et al. |
EMBL Seeing is Believing – Imaging the Molecular Processes of Life
Preprints discussed at the 2019 edition of Seeing is Believing, at EMBL Heidelberg from the 9th-12th October 2019
| List by | Dey Lab |
SDB 78th Annual Meeting 2019
A curation of the preprints presented at the SDB meeting in Boston, July 26-30 2019. The preList will be updated throughout the duration of the meeting.
| List by | Alex Eve |
Lung Disease and Regeneration
This preprint list compiles highlights from the field of lung biology.
| List by | Rob Hynds |
Young Embryologist Network Conference 2019
Preprints presented at the Young Embryologist Network 2019 conference, 13 May, The Francis Crick Institute, London
| List by | Alex Eve |
Pattern formation during development
The aim of this preList is to integrate results about the mechanisms that govern patterning during development, from genes implicated in the processes to theoritical models of pattern formation in nature.
| List by | Alexa Sadier |
BSCB/BSDB Annual Meeting 2019
Preprints presented at the BSCB/BSDB Annual Meeting 2019
| List by | Dey Lab |
Zebrafish immunology
A compilation of cutting-edge research that uses the zebrafish as a model system to elucidate novel immunological mechanisms in health and disease.
| List by | Shikha Nayar |
Also in the genetics category:
BSDB/GenSoc Spring Meeting 2024
A list of preprints highlighted at the British Society for Developmental Biology and Genetics Society joint Spring meeting 2024 at Warwick, UK.
| List by | Joyce Yu, Katherine Brown |
BSCB-Biochemical Society 2024 Cell Migration meeting
This preList features preprints that were discussed and presented during the BSCB-Biochemical Society 2024 Cell Migration meeting in Birmingham, UK in April 2024. Kindly put together by Sara Morais da Silva, Reviews Editor at Journal of Cell Science.
| List by | Reinier Prosee |
9th International Symposium on the Biology of Vertebrate Sex Determination
This preList contains preprints discussed during the 9th International Symposium on the Biology of Vertebrate Sex Determination. This conference was held in Kona, Hawaii from April 17th to 21st 2023.
| List by | Martin Estermann |
Alumni picks – preLights 5th Birthday
This preList contains preprints that were picked and highlighted by preLights Alumni - an initiative that was set up to mark preLights 5th birthday. More entries will follow throughout February and March 2023.
| List by | Sergio Menchero et al. |
Semmelweis Symposium 2022: 40th anniversary of international medical education at Semmelweis University
This preList contains preprints discussed during the 'Semmelweis Symposium 2022' (7-9 November), organised around the 40th anniversary of international medical education at Semmelweis University covering a wide range of topics.
| List by | Nándor Lipták |
20th “Genetics Workshops in Hungary”, Szeged (25th, September)
In this annual conference, Hungarian geneticists, biochemists and biotechnologists presented their works. Link: http://group.szbk.u-szeged.hu/minikonf/archive/prg2021.pdf
| List by | Nándor Lipták |
2nd Conference of the Visegrád Group Society for Developmental Biology
Preprints from the 2nd Conference of the Visegrád Group Society for Developmental Biology (2-5 September, 2021, Szeged, Hungary)
| List by | Nándor Lipták |
EMBL Conference: From functional genomics to systems biology
Preprints presented at the virtual EMBL conference "from functional genomics and systems biology", 16-19 November 2020
| List by | Jesus Victorino |
TAGC 2020
Preprints recently presented at the virtual Allied Genetics Conference, April 22-26, 2020. #TAGC20
| List by | Maiko Kitaoka et al. |
ECFG15 – Fungal biology
Preprints presented at 15th European Conference on Fungal Genetics 17-20 February 2020 Rome
| List by | Hiral Shah |
Autophagy
Preprints on autophagy and lysosomal degradation and its role in neurodegeneration and disease. Includes molecular mechanisms, upstream signalling and regulation as well as studies on pharmaceutical interventions to upregulate the process.
| List by | Sandra Malmgren Hill |