Close

Adult caudal fin shape is imprinted in the embryonic fin fold

Eric Surette, Joan Donahue, Stephanie Robinson, Deirdre McKenna, Crisvely Soto Martinez, Brendan Fitzgerald, Rolf O. Karlstrom, Nicolas Cumplido, Sarah K. McMenamin

Posted on: 28 August 2024

Preprint posted on 19 July 2024

Shhaping the caudal fin: @McmenaminLab describes how shha overexpression induces a novel fin shape in zebrafish

Selected by Isabella Cisneros

Background

The caudal fin, commonly known as the “tail” of a fish, is an important evolutionary innovation found within the ray-finned fishes. Caudal fins can vary widely in their shape due to the swimming ecologies that influence them [1]. However, two different shape classes have emerged from this variation: the truncate shape, where central and peripheral rays (the outer ventral and dorsal rays) have the same length, and the forked fin shape, where peripheral rays are longer than central rays. 

Many studies have focused on deciphering how the caudal fin is patterned. From these, we have been able to define a few key players and mechanisms. When it comes to how the caudal fin is shaped, Hox factors, ion channels and gap junctions, thyroid hormone, and osteoclast activity all play a role [2,3,4]. However, while all of these factors influence different aspects of fin ray growth–such as ray length, identity, growth, and location–the pathways that regulate the shape of the caudal fin remain unknown. 

To get at this question, the authors of this preprint focused on sonic hedgehog (Shh), which is known to regulate limb growth and skeletal identity. Here, the authors show how overexpression of sonic hedgehog a (shha) in the embryonic fin fold can alter overall fin shape and positional memory, further refining our understanding of developmental patterning in the fin and its consequences for processes like regeneration.

 

Main Findings

Transient overexpression of shha during early embryonic development alters overall fin shape

In order to probe the role of shha during fin development, the authors used a transgenic zebrafish line (hsp70l:shha-EGFP) that allowed them to induce transient overexpression of shha using heat shock. They began their experiments by inducing a global pulse at 2 days post fertilization and observing the structure and growth of the larval fin. The authors noticed that, compared to control siblings, zebrafish treated with the shha pulse had significantly longer central rays. This elongation led the fin to adopt a truncate, or flat, shape instead of the usual forked shape. Additionally, the shha pulse also caused the development of pigmented vertical arches in the fin as opposed to the horizontal stripes that usually characterize the forked fin. To ensure that the phenotypes they observed had been mediated through the Shh signaling pathway, the authors inhibited Smoothened, a Shh effector, for a period of 24 or 48 hours following a global shha pulse. These experiments resulted in a partial rescue of the forked fin shape, indicating that disruption of Shh signaling is responsible for the truncate phenotype.

Phenotypic outcomes of shha overexpression depend on developmental stage, fin position, and dose

Next, the authors wanted to determine the developmental stage at which a shha pulse can induce a truncate fin phenotype. To do so, they heat-shocked zebrafish embryos and larvae at multiple timepoints. They found that embryos heat-shocked at 2 or 3 days post fertilization develop truncate fins, but not those at later stages. The authors also wanted to see if a more localized shha pulse could still induce a truncate caudal fin. To test this, they used local heat shock techniques to activate the transgene at two positions along the zebrafish body axis. Here, they found that only activation of Shh at the posterior end of the tail was able to induce the truncate fin phenotype. Next, the authors wanted to test if the amount of shha transgenic activation affected the extent of the truncate fin phenotype. Using GFP brightness and genomic GFP quantity, they were able to predict the severity of the phenotype in relation to the amount, indicating a dose-dependent effect.

Overexpression of shha causes differences in skeletal growth rates and regional cell proliferation in the larval fin

Having characterized the effects of the shha pulses in greater detail, the authors decided to zoom in further into the truncate fin phenotype by analyzing fin ray ossification and hypural chondrogenesis during development. Compared to controls, the shha-pulsed fish lacked a diastema and experienced delayed fin ray growth. To assess whether the truncate fin phenotype is a result of delayed growth, the authors quantified the growth rate between central and peripheral rays in the fin. They found that peripheral rays in shha-pulsed caudal fins grow at the same rate as control siblings. However, this was not the case for central rays, which grow at a 35% faster rate than controls. 

To see if these changes in growth rates corresponded to changes in regional cell proliferation, the authors used a cell-proliferation reporter transgenic line to quantify growth in peripheral and central regions of the fin. Starting at a standard length (a measure of body size) of about 5.9mm, peripheral cell proliferation rates increase compared to those of center regions in control fish. In shha-pulsed fish, however, proliferation in central cell populations increased, leading to the development of a truncate shape in the fin. 

Treatment with shha alters positional memory of the fin in adulthood

Because fin regeneration relies on developmental programs of fin patterning, the authors wanted to see if a shha pulse could also alter memory of fin shape during regeneration. Indeed, this was what they observed: shha-pulsed fish regenerate truncate fins, not forked fins. Additional experiments using longfin and shortfin zebrafish mutants, as well as hypothyroid zebrafish, also resulted in the regeneration of a truncate fin following an embryonic shha pulse. Taking these results together, the authors suggest that, though elements like length, patterning, and shape appear to be regulated by independent signaling pathways, these pathways can be decoupled to generate phenotypic variation in fins.

 

Why I Highlighted This Preprint

As someone who studies caudal fin regeneration, it is always exciting to see work focusing on developmental patterning of the fin–the basis for our work! I find it fascinating that overexpression of sonic hedgehog a can drive such a drastic phenotype. In revealing this new factor’s involvement, this study raises multiple questions related to the cellular and molecular mechanisms driving fin shape patterning during development. It also reveals just how important–and how persistent–embryonic positional memory is for regeneration during adult stages, something to keep in mind for those of us who study this phenomenon.

 

Questions For The Authors

  1. What do you make of the pigmentation defect created by the shha pulse? Is it possible that shha has co-opted a pigmentation patterning role in the fin?
  2. Do you have any plans to test sonic hedgehog b overexpression in additional heat shock experiments? Is there any possibility that shha and shhb have evolutionarily diverged in their patterning roles?

 

References

  1. Giammona, F. Form and Function of the Caudal Fin Throughout the Phylogeny of Fishes, Integrative and Comparative Biology, Volume 61, Issue 2, August 2021, Pages 550–572, https://doi.org/10.1093/icb/icab127
  2. Sakaguchi et al., Medaka unextended-fin mutants suggest a role for Hoxb8a in cell migration and osteoblast differentiation during appendage formation. Dev Biol. 2006 May 15;293(2):42638. https://doi.org/10.1016/j.ydbio.2006.02.017
  3. Stewart et al., longfin causes cis-ectopic expression of the kcnh2a ether-a-go-go K+ channel to autonomously prolong fin outgrowth. Dev Camb Engl. 2021 Jun 1;148(11):dev199384. https://doi.org/10.1242/dev.199384
  4. Harper et al., Thyroid hormone regulates proximodistal patterning in fin rays. Proc Natl Acad Sci. 2023 May 23;120(21):e2219770120. https://doi.org/10.1073/pnas.2219770120
  5. Wang et al., Genetic reprogramming of positional memory in a regenerating appendage. Curr Biol CB. 2019 Dec 16;29(24):4193–4207.e4. https://doi.org/10.1016/j.cub.2019.10.038

Tags: caudal fin, fin development, fin patterning, sonic hedgehog, zebrafish

doi: https://doi.org/10.1242/prelights.38240

Read preprint (No Ratings Yet)

Author's response

Sarah McMenamin shared

1. What do you make of the pigmentation defect created by the shha pulse? Is it possible that shha has co-opted a pigmentation patterning role in the fin?
We actually think that the pigment phenotype is a ’side effect’ of the shifts in developmental timing caused by the shha pulse. Pigment cells end up in the wrong place at the wrong time, and go along for the ride when the notochord flexes upward to reorient the caudal fin tissues posteriorly. These ectopic melanophores set up a horizontal pattern that we believe subsequent stripes follow. (It is also possible that the shh is directly stimulating the pigment cells or directing the pattern, but I think that’s actually less likely).
2. Do you have any plans to test sonic hedgehog b overexpression in additional heat shock experiments? Is there any possibility that shha and shhb have evolutionarily diverged in their patterning roles?
We’ve shown that a pulse of hyperphysiological levels of shha can cause this truncate fin phenotype, but importantly, this doesn’t necessarily imply that shha is essential for normal patterning during these same stages. Indeed, when we transiently block Shh signaling during wild-type larva development, it doesn’t cause much of a phenotype. So to address your second question: it is entirely possible that early overexpression of shhb (or even of other pathways!) might result in similar sorts of phenotypic disruptions by altering the timing of different imprinting processes during fin fold formation.

Have your say

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

Sign up to customise the site to your preferences and to receive alerts

Register here

Also in the cell biology category:

Leukocytes use endothelial membrane tunnels to extravasate the vasculature

Werner J. van der Meer, Abraham C.I. van Steen, Eike Mahlandt, et al.

Selected by 08 December 2024

Felipe Del Valle Batalla

Cell Biology

Platelet-derived LPA16:0 inhibits adult neurogenesis and stress resilience in anxiety disorder

Thomas Larrieu, Charline Carron, Fabio Grieco, et al.

Selected by 04 December 2024

Harvey Roweth

Neuroscience

Investigating Mechanically Activated Currents from Trigeminal Neurons of Non-Human Primates

Karen A Lindquist, Jennifer Mecklenburg, Anahit H. Hovhannisyan, et al.

Selected by 04 December 2024

Vanessa Ehlers

Neuroscience

Also in the developmental biology category:

Cellular signalling protrusions enable dynamic distant contacts in spinal cord neurogenesis

Joshua Hawley, Robert Lea, Veronica Biga, et al.

Selected by 15 November 2024

Ankita Walvekar

Developmental Biology

Actin-based deformations of the nucleus control multiciliated ependymal cell differentiation

Marianne Basso, Alexia Mahuzier, Syed Kaabir Ali, et al.

Selected by 30 October 2024

Ryan Harrison

Developmental Biology

HIF1A contributes to the survival of aneuploid and mosaic pre-implantation embryos

Estefania Sanchez-Vasquez, Marianne E. Bronner, Magdalena Zernicka-Goetz

Selected by 11 October 2024

Anchel De Jaime Soguero

Developmental Biology

Also in the molecular biology category:

Platelet-derived LPA16:0 inhibits adult neurogenesis and stress resilience in anxiety disorder

Thomas Larrieu, Charline Carron, Fabio Grieco, et al.

Selected by 04 December 2024

Harvey Roweth

Neuroscience

Green synthesized silver nanoparticles from Moringa: Potential for preventative treatment of SARS-CoV-2 contaminated water

Adebayo J. Bello, Omorilewa B. Ebunoluwa, Rukayat O. Ayorinde, et al.

Selected by 14 November 2024

Safieh Shah, Benjamin Dominik Maier

Epidemiology

Non-disruptive inducible labeling of ER-membrane contact sites using the Lamin B Receptor

Laura Downie, Nuria Ferrandiz, Megan Jones, et al.

Selected by 15 October 2024

Jonathan Townson

Cell Biology

preLists in the cell biology category:

November in preprints – the CellBio edition

This is the first community-driven preList! A group of preLighters, with expertise in different areas of cell biology, have worked together to create this preprint reading lists for researchers with an interest in cell biology. Categories include: 1) cancer cell biology 2) cell cycle and division 3) cell migration and cytoskeleton 4) cell organelles and organisation 5) cell signalling and mechanosensing 6) genetics/gene expression

 



List by Felipe Del Valle Batalla et al.

BSCB-Biochemical Society 2024 Cell Migration meeting

This preList features preprints that were discussed and presented during the BSCB-Biochemical Society 2024 Cell Migration meeting in Birmingham, UK in April 2024. Kindly put together by Sara Morais da Silva, Reviews Editor at Journal of Cell Science.

 



List by Reinier Prosee

‘In preprints’ from Development 2022-2023

A list of the preprints featured in Development's 'In preprints' articles between 2022-2023

 



List by Alex Eve, Katherine Brown

preLights peer support – preprints of interest

This is a preprint repository to organise the preprints and preLights covered through the 'preLights peer support' initiative.

 



List by preLights peer support

The Society for Developmental Biology 82nd Annual Meeting

This preList is made up of the preprints discussed during the Society for Developmental Biology 82nd Annual Meeting that took place in Chicago in July 2023.

 



List by Joyce Yu, Katherine Brown

CSHL 87th Symposium: Stem Cells

Preprints mentioned by speakers at the #CSHLsymp23

 



List by Alex Eve

Journal of Cell Science meeting ‘Imaging Cell Dynamics’

This preList highlights the preprints discussed at the JCS meeting 'Imaging Cell Dynamics'. The meeting was held from 14 - 17 May 2023 in Lisbon, Portugal and was organised by Erika Holzbaur, Jennifer Lippincott-Schwartz, Rob Parton and Michael Way.

 



List by Helen Zenner

9th International Symposium on the Biology of Vertebrate Sex Determination

This preList contains preprints discussed during the 9th International Symposium on the Biology of Vertebrate Sex Determination. This conference was held in Kona, Hawaii from April 17th to 21st 2023.

 



List by Martin Estermann

Alumni picks – preLights 5th Birthday

This preList contains preprints that were picked and highlighted by preLights Alumni - an initiative that was set up to mark preLights 5th birthday. More entries will follow throughout February and March 2023.

 



List by Sergio Menchero et al.

CellBio 2022 – An ASCB/EMBO Meeting

This preLists features preprints that were discussed and presented during the CellBio 2022 meeting in Washington, DC in December 2022.

 



List by Nadja Hümpfer et al.

Fibroblasts

The advances in fibroblast biology preList explores the recent discoveries and preprints of the fibroblast world. Get ready to immerse yourself with this list created for fibroblasts aficionados and lovers, and beyond. Here, my goal is to include preprints of fibroblast biology, heterogeneity, fate, extracellular matrix, behavior, topography, single-cell atlases, spatial transcriptomics, and their matrix!

 



List by Osvaldo Contreras

EMBL Synthetic Morphogenesis: From Gene Circuits to Tissue Architecture (2021)

A list of preprints mentioned at the #EESmorphoG virtual meeting in 2021.

 



List by Alex Eve

FENS 2020

A collection of preprints presented during the virtual meeting of the Federation of European Neuroscience Societies (FENS) in 2020

 



List by Ana Dorrego-Rivas

Planar Cell Polarity – PCP

This preList contains preprints about the latest findings on Planar Cell Polarity (PCP) in various model organisms at the molecular, cellular and tissue levels.

 



List by Ana Dorrego-Rivas

BioMalPar XVI: Biology and Pathology of the Malaria Parasite

[under construction] Preprints presented at the (fully virtual) EMBL BioMalPar XVI, 17-18 May 2020 #emblmalaria

 



List by Dey Lab, Samantha Seah

1

Cell Polarity

Recent research from the field of cell polarity is summarized in this list of preprints. It comprises of studies focusing on various forms of cell polarity ranging from epithelial polarity, planar cell polarity to front-to-rear polarity.

 



List by Yamini Ravichandran

TAGC 2020

Preprints recently presented at the virtual Allied Genetics Conference, April 22-26, 2020. #TAGC20

 



List by Maiko Kitaoka et al.

3D Gastruloids

A curated list of preprints related to Gastruloids (in vitro models of early development obtained by 3D aggregation of embryonic cells). Updated until July 2021.

 



List by Paul Gerald L. Sanchez and Stefano Vianello

ECFG15 – Fungal biology

Preprints presented at 15th European Conference on Fungal Genetics 17-20 February 2020 Rome

 



List by Hiral Shah

ASCB EMBO Annual Meeting 2019

A collection of preprints presented at the 2019 ASCB EMBO Meeting in Washington, DC (December 7-11)

 



List by Madhuja Samaddar et al.

EMBL Seeing is Believing – Imaging the Molecular Processes of Life

Preprints discussed at the 2019 edition of Seeing is Believing, at EMBL Heidelberg from the 9th-12th October 2019

 



List by Dey Lab

Autophagy

Preprints on autophagy and lysosomal degradation and its role in neurodegeneration and disease. Includes molecular mechanisms, upstream signalling and regulation as well as studies on pharmaceutical interventions to upregulate the process.

 



List by Sandra Malmgren Hill

Lung Disease and Regeneration

This preprint list compiles highlights from the field of lung biology.

 



List by Rob Hynds

Cellular metabolism

A curated list of preprints related to cellular metabolism at Biorxiv by Pablo Ranea Robles from the Prelights community. Special interest on lipid metabolism, peroxisomes and mitochondria.

 



List by Pablo Ranea Robles

BSCB/BSDB Annual Meeting 2019

Preprints presented at the BSCB/BSDB Annual Meeting 2019

 



List by Dey Lab

MitoList

This list of preprints is focused on work expanding our knowledge on mitochondria in any organism, tissue or cell type, from the normal biology to the pathology.

 



List by Sandra Franco Iborra

Biophysical Society Annual Meeting 2019

Few of the preprints that were discussed in the recent BPS annual meeting at Baltimore, USA

 



List by Joseph Jose Thottacherry

ASCB/EMBO Annual Meeting 2018

This list relates to preprints that were discussed at the recent ASCB conference.

 



List by Dey Lab, Amanda Haage

Also in the developmental biology category:

BSDB/GenSoc Spring Meeting 2024

A list of preprints highlighted at the British Society for Developmental Biology and Genetics Society joint Spring meeting 2024 at Warwick, UK.

 



List by Joyce Yu, Katherine Brown

GfE/ DSDB meeting 2024

This preList highlights the preprints discussed at the 2024 joint German and Dutch developmental biology societies meeting that took place in March 2024 in Osnabrück, Germany.

 



List by Joyce Yu

‘In preprints’ from Development 2022-2023

A list of the preprints featured in Development's 'In preprints' articles between 2022-2023

 



List by Alex Eve, Katherine Brown

preLights peer support – preprints of interest

This is a preprint repository to organise the preprints and preLights covered through the 'preLights peer support' initiative.

 



List by preLights peer support

The Society for Developmental Biology 82nd Annual Meeting

This preList is made up of the preprints discussed during the Society for Developmental Biology 82nd Annual Meeting that took place in Chicago in July 2023.

 



List by Joyce Yu, Katherine Brown

CSHL 87th Symposium: Stem Cells

Preprints mentioned by speakers at the #CSHLsymp23

 



List by Alex Eve

Journal of Cell Science meeting ‘Imaging Cell Dynamics’

This preList highlights the preprints discussed at the JCS meeting 'Imaging Cell Dynamics'. The meeting was held from 14 - 17 May 2023 in Lisbon, Portugal and was organised by Erika Holzbaur, Jennifer Lippincott-Schwartz, Rob Parton and Michael Way.

 



List by Helen Zenner

9th International Symposium on the Biology of Vertebrate Sex Determination

This preList contains preprints discussed during the 9th International Symposium on the Biology of Vertebrate Sex Determination. This conference was held in Kona, Hawaii from April 17th to 21st 2023.

 



List by Martin Estermann

Alumni picks – preLights 5th Birthday

This preList contains preprints that were picked and highlighted by preLights Alumni - an initiative that was set up to mark preLights 5th birthday. More entries will follow throughout February and March 2023.

 



List by Sergio Menchero et al.

CellBio 2022 – An ASCB/EMBO Meeting

This preLists features preprints that were discussed and presented during the CellBio 2022 meeting in Washington, DC in December 2022.

 



List by Nadja Hümpfer et al.

2nd Conference of the Visegrád Group Society for Developmental Biology

Preprints from the 2nd Conference of the Visegrád Group Society for Developmental Biology (2-5 September, 2021, Szeged, Hungary)

 



List by Nándor Lipták

Fibroblasts

The advances in fibroblast biology preList explores the recent discoveries and preprints of the fibroblast world. Get ready to immerse yourself with this list created for fibroblasts aficionados and lovers, and beyond. Here, my goal is to include preprints of fibroblast biology, heterogeneity, fate, extracellular matrix, behavior, topography, single-cell atlases, spatial transcriptomics, and their matrix!

 



List by Osvaldo Contreras

EMBL Synthetic Morphogenesis: From Gene Circuits to Tissue Architecture (2021)

A list of preprints mentioned at the #EESmorphoG virtual meeting in 2021.

 



List by Alex Eve

EMBL Conference: From functional genomics to systems biology

Preprints presented at the virtual EMBL conference "from functional genomics and systems biology", 16-19 November 2020

 



List by Jesus Victorino

Single Cell Biology 2020

A list of preprints mentioned at the Wellcome Genome Campus Single Cell Biology 2020 meeting.

 



List by Alex Eve

Society for Developmental Biology 79th Annual Meeting

Preprints at SDB 2020

 



List by Irepan Salvador-Martinez, Martin Estermann

FENS 2020

A collection of preprints presented during the virtual meeting of the Federation of European Neuroscience Societies (FENS) in 2020

 



List by Ana Dorrego-Rivas

Planar Cell Polarity – PCP

This preList contains preprints about the latest findings on Planar Cell Polarity (PCP) in various model organisms at the molecular, cellular and tissue levels.

 



List by Ana Dorrego-Rivas

Cell Polarity

Recent research from the field of cell polarity is summarized in this list of preprints. It comprises of studies focusing on various forms of cell polarity ranging from epithelial polarity, planar cell polarity to front-to-rear polarity.

 



List by Yamini Ravichandran

TAGC 2020

Preprints recently presented at the virtual Allied Genetics Conference, April 22-26, 2020. #TAGC20

 



List by Maiko Kitaoka et al.

3D Gastruloids

A curated list of preprints related to Gastruloids (in vitro models of early development obtained by 3D aggregation of embryonic cells). Updated until July 2021.

 



List by Paul Gerald L. Sanchez and Stefano Vianello

ASCB EMBO Annual Meeting 2019

A collection of preprints presented at the 2019 ASCB EMBO Meeting in Washington, DC (December 7-11)

 



List by Madhuja Samaddar et al.

EDBC Alicante 2019

Preprints presented at the European Developmental Biology Congress (EDBC) in Alicante, October 23-26 2019.

 



List by Sergio Menchero et al.

EMBL Seeing is Believing – Imaging the Molecular Processes of Life

Preprints discussed at the 2019 edition of Seeing is Believing, at EMBL Heidelberg from the 9th-12th October 2019

 



List by Dey Lab

SDB 78th Annual Meeting 2019

A curation of the preprints presented at the SDB meeting in Boston, July 26-30 2019. The preList will be updated throughout the duration of the meeting.

 



List by Alex Eve

Lung Disease and Regeneration

This preprint list compiles highlights from the field of lung biology.

 



List by Rob Hynds

Young Embryologist Network Conference 2019

Preprints presented at the Young Embryologist Network 2019 conference, 13 May, The Francis Crick Institute, London

 



List by Alex Eve

Pattern formation during development

The aim of this preList is to integrate results about the mechanisms that govern patterning during development, from genes implicated in the processes to theoritical models of pattern formation in nature.

 



List by Alexa Sadier

BSCB/BSDB Annual Meeting 2019

Preprints presented at the BSCB/BSDB Annual Meeting 2019

 



List by Dey Lab

Zebrafish immunology

A compilation of cutting-edge research that uses the zebrafish as a model system to elucidate novel immunological mechanisms in health and disease.

 



List by Shikha Nayar

Also in the molecular biology category:

2024 Hypothalamus GRC

This 2024 Hypothalamus GRC (Gordon Research Conference) preList offers an overview of cutting-edge research focused on the hypothalamus, a critical brain region involved in regulating homeostasis, behavior, and neuroendocrine functions. The studies included cover a range of topics, including neural circuits, molecular mechanisms, and the role of the hypothalamus in health and disease. This collection highlights some of the latest advances in understanding hypothalamic function, with potential implications for treating disorders such as obesity, stress, and metabolic diseases.

 



List by Nathalie Krauth

BSCB-Biochemical Society 2024 Cell Migration meeting

This preList features preprints that were discussed and presented during the BSCB-Biochemical Society 2024 Cell Migration meeting in Birmingham, UK in April 2024. Kindly put together by Sara Morais da Silva, Reviews Editor at Journal of Cell Science.

 



List by Reinier Prosee

‘In preprints’ from Development 2022-2023

A list of the preprints featured in Development's 'In preprints' articles between 2022-2023

 



List by Alex Eve, Katherine Brown

CSHL 87th Symposium: Stem Cells

Preprints mentioned by speakers at the #CSHLsymp23

 



List by Alex Eve

9th International Symposium on the Biology of Vertebrate Sex Determination

This preList contains preprints discussed during the 9th International Symposium on the Biology of Vertebrate Sex Determination. This conference was held in Kona, Hawaii from April 17th to 21st 2023.

 



List by Martin Estermann

Alumni picks – preLights 5th Birthday

This preList contains preprints that were picked and highlighted by preLights Alumni - an initiative that was set up to mark preLights 5th birthday. More entries will follow throughout February and March 2023.

 



List by Sergio Menchero et al.

CellBio 2022 – An ASCB/EMBO Meeting

This preLists features preprints that were discussed and presented during the CellBio 2022 meeting in Washington, DC in December 2022.

 



List by Nadja Hümpfer et al.

EMBL Synthetic Morphogenesis: From Gene Circuits to Tissue Architecture (2021)

A list of preprints mentioned at the #EESmorphoG virtual meeting in 2021.

 



List by Alex Eve

FENS 2020

A collection of preprints presented during the virtual meeting of the Federation of European Neuroscience Societies (FENS) in 2020

 



List by Ana Dorrego-Rivas

ECFG15 – Fungal biology

Preprints presented at 15th European Conference on Fungal Genetics 17-20 February 2020 Rome

 



List by Hiral Shah

ASCB EMBO Annual Meeting 2019

A collection of preprints presented at the 2019 ASCB EMBO Meeting in Washington, DC (December 7-11)

 



List by Madhuja Samaddar et al.

Lung Disease and Regeneration

This preprint list compiles highlights from the field of lung biology.

 



List by Rob Hynds

MitoList

This list of preprints is focused on work expanding our knowledge on mitochondria in any organism, tissue or cell type, from the normal biology to the pathology.

 



List by Sandra Franco Iborra
Close