Close
Close

preLights is supported by…

Related posts

Related preLists

Structural basis of respiratory complexes adaptation to cold temperatures

Young-Cheul Shin, Pedro Latorre-Muro, Amina Djurabekova, Oleksii Zdorevskyi, Christopher F. Bennett, Nils Burger, Kangkang Song, Chen Xu, Vivek Sharma, Maofu Liao, Pere Puigserver

Posted on: 10 April 2024

Preprint posted on 17 January 2024

Discover how organisms adapt to cold weather! Shin and Latorre-Muro et al. unveil a mitochondrial response to low temperatures in their comprehensive study of respiration and heat generation.

Selected by Pamela Ornelas

Categories: biochemistry, biophysics, cell biology

Background

When exposed to cold environments, mammals activate brown adipocyte cells (BAs) to generate heat and regulate body temperature (1). BAs, dense with mitochondrial cristae, undergo deep protein and lipid remodeling to sustain heightened oxidation rates of glucose and fatty acids in response to low temperatures (2). This adaptation mechanism relies on the electron transport chain (ETC) within mitochondria, which generates a proton motive force that can be used to release energy in the form of heat (3,4). However, the structural basis of cold-induced effects on respiratory complexes is not well understood.

In their preprint, Shin, Latorre-Muro, and colleagues combined thermoregulatory physiology and cryo-electron microscopy (cryoEM) to investigate respiratory supercomplexes exposed to different temperatures. They identified a cold-induced conformation within the CI:III2 supercomplex, elucidating the unique lipid-protein arrangements that stabilize it.

Key findings

Exposure to cold temperatures

Shin, Latorre-Muro, and colleagues compared brown adipose tissue samples from wild-type and model mice exposed to varying temperatures over 8 days. After mitochondrial solubilization, respiratory supercomplexes were purified through size exclusion chromatography, with subsequent activity determination. Peak fractions were analyzed through single particle cryoEM, resulting in high-resolution maps of the CI:III2 supercomplex across different temperatures. This revealed a conformational change of the respiratory complex as a cold adaptation response.

Respiratory complex remodeling

The study resolved two conformations of the CI:III2 supercomplex. Thermoneutral mouse samples presented only a canonical (type 1) conformation, while a structurally different second conformation (type 2) was also observed in samples of cold-acclimated mice. This suggests that in response to cold temperatures, type 1 respiratory complexes transit to the type 2 conformation. The difference between these conformations lies in the 25˚ rearrangement of the CIII2 axis, perpendicular to the CI membrane domain, resulting in an open angle between the longitudinal CI membrane domain and the CIII2 transversal axes.

Lipids play an important role

Given that lipid remodeling in mitochondrial membranes increases ETC activity (5), Shin, Latorre-Muro, and colleagues conducted lipidomics analyses on isolated mitochondria. They found a distinct enrichment in unsaturated phosphatidylethanolamine (PE) and phosphatidylcholine (PC) in tissues exposed to cold and neutral temperatures. Molecular dynamics simulations revealed increased lipid density at the CI/CIII2 interface in the type 2 supercomplex compared to type 1. On this basis, the conformational transition from type 1 to type 2 under cold conditions is proposed to occur by lipid remodeling (PC/PE), increasing membrane flexibility and allowing the accommodation of type 2 complexes to straightened cristae. This results in a lower energy barrier compared to thermoneutral conditions.

In summary, mammalian survival in cold temperatures involves remodeling of brown fat mitochondrial proteins. The authors propose that upon rotation of CIII2, type 2 assemblies become highly active and increase respiration, supporting heat production and maintaining body temperature.

Why is this important?

This preprint provides a great insight into the dynamics of respiratory adaptation under low temperatures. The work presented by Shin, Latorre-Muro, and their colleagues is comprehensive, as they study adipose tissue under different genetic and environmental conditions using cryo-EM, molecular dynamic simulations, and biochemical analyses. This research offers a fresh perspective on cellular temperature regulation and metabolic response to external factors.

I was particularly interested in this work for personal reasons. Every winter, my structural biology friends and I challenge ourselves to swim a nearby lake on Sunday mornings. Our short submersions in near 0˚ C water often prompt discussions about our body’s amazing capacity to adapt to the cold and have sparked our curiosity about metabolism in extreme weather conditions.

 

Questions to the authors

    1. Do you expect an increase in mitochondrial protein and lipid import under cold-induced stress to make up for the cristae rearrangement? Are there specific regulatory factors involved in this process?
    2. Based on your molecular dynamics simulations, can you predict the speed of the conformational change between type 1 and 2 assemblies?
    3. Would you expect similar reorganization in other respiratory supercomplexes to maximize electron transfer?
    4. Are there shared mechanisms used by prokaryotes to maintain energy production under cold temperatures?

 

References:

    1. Cannon, J. Nedergaard, Brown adipose tissue: function and physiological significance. Physiol Rev 84, 277–359 (2004).
    2. Orava, P. Nuutila, M. E. Lidell, V. Oikonen, T. Noponen, T. Viljanen, M. Scheinin, M. Taittonen, T. Niemi, S. Enerbäck, K. A. Virtanen, Different Metabolic Responses of Human Brown Adipose Tissue to Activation by Cold and Insulin. Cell Metab 14, 272–279 (2011).
    3. Enerbäck, A. Jacobsson, E. M. Simpson, C. Guerra, H. Yamashita, M.-E. Harper, L. P. Kozak, Mice lacking mitochondrial uncoupling protein are cold-sensitive but not obese. Nature 387, 90–94 (1997).
    4. Kazak, B. M. Spiegelman, Mechanism of futile creatine cycling in thermogenesis. Am J Physiol Endocrinol Metab 319, E947–E949 (2020).
    5. G. Sustarsic, T. Ma, M. D. Lynes, M. Larsen, I. Karavaeva, J. F. Havelund, C. H. Nielsen, M. P. Jedrychowski, M. Moreno-Torres, M. Lundh, K. Plucinska, N. Z. Jespersen, T. J. Grevengoed, B. Kramar, J. Peics, J. B. Hansen, F. Shamsi, I. Forss, D. Neess, S. Keipert, J. Wang, K. Stohlmann, I. Brandslund, C. Christensen, M. E. Jørgensen, A. Linneberg, O. Pedersen, M. A. Kiebish, K. Qvortrup, X. Han, B. K. Pedersen, M. Jastroch, S. Mandrup, A. Kjær, S. P. Gygi, T. Hansen, M. P. Gillum, N. Grarup, B. Emanuelli, S. Nielsen, C. Scheele, Y. H. Tseng, N. J. Færgeman, Z. Gerhart-Hines, Cardiolipin Synthesis in Brown and Beige Fat Mitochondria Is Essential for Systemic Energy Homeostasis. Cell Metab 28, 159-174.e11 (2018).

Tags: cryoem, mitochondria, respiration, respiratory complex

doi: https://doi.org/10.1242/prelights.37071

Read preprint (No Ratings Yet)

Author's response

The author team shared

  1. Do you expect an increase in mitochondrial protein and lipid import under cold-induced stress to make up for the cristae rearrangement? Are there specific regulatory factors involved in this process?

R: There is a growing interest in the understanding of organelle remodeling in response to environmental factors. We have previously reported that, during cold acclimation, the activity of mitochondrial import machineries, and more specifically, the receptor TOM70, regulates the mitochondrial protein import of at least the cristae component MIC19(1). These results show how cristae remodeling impacts the capability of brown adipocytes to adapt to cold environments. This process is regulated by the PERK branch of the Integrated Stress Response (ISR) through a non-canonical pathway and indicates that protein remodeling is necessary for successful cold acclimation. In addition, we have seen that MIC19 overexpression in livers can protect against diet-induced obesity, further supporting the connection between mitochondria cristae and metabolic adaptation(2). Based on these findings, it seems feasible that protein and lipid remodeling converge together.  Previous literature and our results suggest that lipid remodeling during cold acclimation enriches the presence of unsaturated PC and PE species(3), which aid the stabilization of respiratory supercomplexes(4). Yet, further research is necessary to investigate how these lipids are generated and delivered to mitochondria.

 

  1. Based on your molecular dynamics simulations, can you predict the speed of the conformational change between type 1 and 2 assemblies?

R: This is a very good question, and we would indeed like to know about the energy barriers that separate the two states. This may tell us about the rates of conversion between two states. However, this is a complex process, and this scenario involves many players – protein and lipid dynamics, lipid composition, and several other factors involving mitochondrial membrane. Treating such aspects in a coupled fashion through modeling and simulation methods alone is a current challenge.

 

  1. Would you expect similar reorganization in other respiratory supercomplexes to maximize electron transfer?

R: The initial description of type 2 complexes is from 2016 using samples from ovine hearts(5). A second paper in 2022 described a rotation of CIII2:IV relative to CI in swine hearts(6). Yet, EM maps were obtained at 8-10Å, likely due to the low stoichiometry of these particles. These works indicate that in tissues other than brown adipose tissue, type 2 complexes exist. Our work addresses the question of the existence of these rotated complexes by employing mouse models under very controlled environmental conditions, thermoneutrality and cold-acclimation. These conditions represent the minimum and maximum metabolic level of activity in brown adipocytes, respectively. By clearly segregating the metabolic state, we were able to maximize the number of particles with a rotated conformation in our samples and obtain high-resolution maps. The structural information allowed us to calculate the behavior of these assemblies by molecular dynamics simulations that suggest, along with the biochemical data, that type 2 complexes maintain the system in a high-energy state. Therefore, the rearrangement of respiratory complexes from type 1 to highly active type 2 is a general process that can reign across tissues in response to environmental cues to satisfy cellular energetic demands.

 

  1. Are there shared mechanisms used by prokaryotes to maintain energy production under cold temperatures?

R: That is a very interesting question. Prokaryotes slow down their metabolism under cold conditions. Some bacteria show optimal growth rates at 10-20°C instead of the standard 37°C. However, during recent years, several works have observed correlation between cristae morphology and respiratory complex structure across kingdoms(7–10). This implies that the different forms of life adapt their respiratory machineries to satisfy their energetic needs in response to environmental cues.

 

  1. P. Latorre-Muro, K. E. O’Malley, C. F. Bennett, E. A. Perry, E. Balsa, C. D. J. Tavares, M. Jedrychowski, S. P. Gygi, P. Puigserver, A cold-stress-inducible PERK/OGT axis controls TOM70-assisted mitochondrial protein import and cristae formation. Cell Metab 33, 598-614.e7 (2021).
  2. J. H. Sohn, B. Mutlu, P. Latorre-Muro, J. Liang, C. F. Bennett, K. Sharabi, N. Kantorovich, M. Jedrychowski, S. P. Gygi, A. S. Banks, P. Puigserver, Liver mitochondrial cristae organizing protein MIC19 promotes energy expenditure and pedestrian locomotion by altering nucleotide metabolism. Cell Metab 35, 1356–1372 (2023).
  3. E. G. Sustarsic, T. Ma, M. D. Lynes, M. Larsen, I. Karavaeva, J. F. Havelund, C. H. Nielsen, M. P. Jedrychowski, M. Moreno-Torres, M. Lundh, K. Plucinska, N. Z. Jespersen, T. J. Grevengoed, B. Kramar, J. Peics, J. B. Hansen, F. Shamsi, I. Forss, D. Neess, S. Keipert, J. Wang, K. Stohlmann, I. Brandslund, C. Christensen, M. E. Jørgensen, A. Linneberg, O. Pedersen, M. A. Kiebish, K. Qvortrup, X. Han, B. K. Pedersen, M. Jastroch, S. Mandrup, A. Kjær, S. P. Gygi, T. Hansen, M. P. Gillum, N. Grarup, B. Emanuelli, S. Nielsen, C. Scheele, Y. H. Tseng, N. J. Færgeman, Z. Gerhart-Hines, Cardiolipin Synthesis in Brown and Beige Fat Mitochondria Is Essential for Systemic Energy Homeostasis. Cell Metab 28, 159-174.e11 (2018).
  4. C. F. Bennett, K. E. O’Malley, E. A. Perry, E. Balsa, P. Latorre-Muro, C. L. Riley, C. Luo, M. Jedrychowski, S. P. Gygi, P. Puigserver, Peroxisomal-derived ether phospholipids link nucleotides to respirasome assembly. Nat Chem Biol 17, 703–710 (2021).
  5. J. S. Sousa, D. J. Mills, J. Vonck, W. Kühlbrandt, Functional asymmetry and electron flow in the bovine respirasome. Elife 5, e21290 (2016).
  6. T. J. Jeon, S.-G. Lee, S. H. Yoo, M. Kim, D. Song, J. Ryu, H. Park, D.-S. Kim, J. Hyun, H. M. Kim, S. E. Ryu, A Dynamic Substrate Pool Revealed by cryo-EM of a Lipid-Preserved Respiratory Supercomplex. Antioxid Redox Signal 36, 1101–1118 (2022).
  7. L. Zhou, M. Maldonado, A. Padavannil, F. Guo, J. A. Letts, Structures of Tetrahymena’s respiratory chain reveal the diversity of eukaryotic core metabolism. Science 376, 831–839 (2022).
  8. N. Klusch, M. Dreimann, J. Senkler, N. Rugen, W. Kühlbrandt, H.-P. Braun, Cryo-EM structure of the respiratory I + III2 supercomplex from Arabidopsis thaliana at 2 Å resolution. Nat Plants 9, 142–156 (2022).
  9. M. Maldonado, Z. Fan, K. M. Abe, J. A. Letts, Plant-specific features of respiratory supercomplex I + III2 from Vigna radiata. Nat Plants 9, 157–168 (2022).
  10. A. Mühleip, R. K. Flygaard, R. Baradaran, O. Haapanen, T. Gruhl, V. Tobiasson, A. Maréchal, V. Sharma, A. Amunts, Structural basis of mitochondrial membrane bending by the I–II–III2–IV2 supercomplex. Nature 615, 934–938 (2023).

 

Have your say

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

Sign up to customise the site to your preferences and to receive alerts

Register here

Also in the biochemistry category:

Triglyceride metabolism controls inflammation and APOE4-associated disease states in microglia

Roxan A. Stephenson, Kory R. Johnson, Linling Cheng, et al.

Selected by 22 August 2024

Gustavo Stelzer, Marcus Oliveira

Biochemistry

Impaired 26S proteasome causes learning and memory deficiency and induces neuroinflammation mediated by NF-κB in mice

Christa C. Huber, Eduardo Callegari, Maria Paez, et al.

Selected by 22 August 2024

Gustavo Stelzer, Marcus Oliveira

Biochemistry

Notch3 is a genetic modifier of NODAL signalling for patterning asymmetry during mouse heart looping

Tobias Holm Bønnelykke, Marie-Amandine Chabry, Emeline Perthame, et al.

Selected by 06 June 2024

Bhaval Parmar

Developmental Biology

Also in the biophysics category:

Motor Clustering Enhances Kinesin-driven Vesicle Transport

Rui Jiang, Qingzhou Feng, Daguan Nong, et al.

Selected by 16 November 2024

Sharvari Pitke

Biophysics

Global coordination of protrusive forces in migrating immune cells

Patricia Reis-Rodrigues, Nikola Canigova, Mario J. Avellaneda, et al.

Selected by 10 October 2024

yohalie kalukula

Biophysics

Engineered Nanotopographies Induce Transient Openings in the Nuclear Membrane

Einollah Sarikhani, Vrund Patel, Zhi Li, et al.

Selected by 23 September 2024

Sristilekha Nath

Bioengineering

Also in the cell biology category:

Motor Clustering Enhances Kinesin-driven Vesicle Transport

Rui Jiang, Qingzhou Feng, Daguan Nong, et al.

Selected by 16 November 2024

Sharvari Pitke

Biophysics

Cellular signalling protrusions enable dynamic distant contacts in spinal cord neurogenesis

Joshua Hawley, Robert Lea, Veronica Biga, et al.

Selected by 15 November 2024

Ankita Walvekar

Developmental Biology

Green synthesized silver nanoparticles from Moringa: Potential for preventative treatment of SARS-CoV-2 contaminated water

Adebayo J. Bello, Omorilewa B. Ebunoluwa, Rukayat O. Ayorinde, et al.

Selected by 14 November 2024

Safieh Shah, Benjamin Dominik Maier

Epidemiology

preLists in the biochemistry category:

BSCB-Biochemical Society 2024 Cell Migration meeting

This preList features preprints that were discussed and presented during the BSCB-Biochemical Society 2024 Cell Migration meeting in Birmingham, UK in April 2024. Kindly put together by Sara Morais da Silva, Reviews Editor at Journal of Cell Science.

 



List by Reinier Prosee

Peer Review in Biomedical Sciences

Communication of scientific knowledge has changed dramatically in recent decades and the public perception of scientific discoveries depends on the peer review process of articles published in scientific journals. Preprints are key vehicles for the dissemination of scientific discoveries, but they are still not properly recognized by the scientific community since peer review is very limited. On the other hand, peer review is very heterogeneous and a fundamental aspect to improve it is to train young scientists on how to think critically and how to evaluate scientific knowledge in a professional way. Thus, this course aims to: i) train students on how to perform peer review of scientific manuscripts in a professional manner; ii) develop students' critical thinking; iii) contribute to the appreciation of preprints as important vehicles for the dissemination of scientific knowledge without restrictions; iv) contribute to the development of students' curricula, as their opinions will be published and indexed on the preLights platform. The evaluations will be based on qualitative analyses of the oral presentations of preprints in the field of biomedical sciences deposited in the bioRxiv server, of the critical reports written by the students, as well as of the participation of the students during the preprints discussions.

 



List by Marcus Oliveira et al.

CellBio 2022 – An ASCB/EMBO Meeting

This preLists features preprints that were discussed and presented during the CellBio 2022 meeting in Washington, DC in December 2022.

 



List by Nadja Hümpfer et al.

20th “Genetics Workshops in Hungary”, Szeged (25th, September)

In this annual conference, Hungarian geneticists, biochemists and biotechnologists presented their works. Link: http://group.szbk.u-szeged.hu/minikonf/archive/prg2021.pdf

 



List by Nándor Lipták

Fibroblasts

The advances in fibroblast biology preList explores the recent discoveries and preprints of the fibroblast world. Get ready to immerse yourself with this list created for fibroblasts aficionados and lovers, and beyond. Here, my goal is to include preprints of fibroblast biology, heterogeneity, fate, extracellular matrix, behavior, topography, single-cell atlases, spatial transcriptomics, and their matrix!

 



List by Osvaldo Contreras

ASCB EMBO Annual Meeting 2019

A collection of preprints presented at the 2019 ASCB EMBO Meeting in Washington, DC (December 7-11)

 



List by Madhuja Samaddar et al.

EMBL Seeing is Believing – Imaging the Molecular Processes of Life

Preprints discussed at the 2019 edition of Seeing is Believing, at EMBL Heidelberg from the 9th-12th October 2019

 



List by Dey Lab

Cellular metabolism

A curated list of preprints related to cellular metabolism at Biorxiv by Pablo Ranea Robles from the Prelights community. Special interest on lipid metabolism, peroxisomes and mitochondria.

 



List by Pablo Ranea Robles

MitoList

This list of preprints is focused on work expanding our knowledge on mitochondria in any organism, tissue or cell type, from the normal biology to the pathology.

 



List by Sandra Franco Iborra

Also in the biophysics category:

preLights peer support – preprints of interest

This is a preprint repository to organise the preprints and preLights covered through the 'preLights peer support' initiative.

 



List by preLights peer support

66th Biophysical Society Annual Meeting, 2022

Preprints presented at the 66th BPS Annual Meeting, Feb 19 - 23, 2022 (The below list is not exhaustive and the preprints are listed in no particular order.)

 



List by Soni Mohapatra

EMBL Synthetic Morphogenesis: From Gene Circuits to Tissue Architecture (2021)

A list of preprints mentioned at the #EESmorphoG virtual meeting in 2021.

 



List by Alex Eve

Biophysical Society Meeting 2020

Some preprints presented at the Biophysical Society Meeting 2020 in San Diego, USA.

 



List by Tessa Sinnige

ASCB EMBO Annual Meeting 2019

A collection of preprints presented at the 2019 ASCB EMBO Meeting in Washington, DC (December 7-11)

 



List by Madhuja Samaddar et al.

EMBL Seeing is Believing – Imaging the Molecular Processes of Life

Preprints discussed at the 2019 edition of Seeing is Believing, at EMBL Heidelberg from the 9th-12th October 2019

 



List by Dey Lab

Biomolecular NMR

Preprints related to the application and development of biomolecular NMR spectroscopy

 



List by Reid Alderson

Biophysical Society Annual Meeting 2019

Few of the preprints that were discussed in the recent BPS annual meeting at Baltimore, USA

 



List by Joseph Jose Thottacherry

Also in the cell biology category:

November in preprints – the CellBio edition

This is the first community-driven preList! A group of preLighters, with expertise in different areas of cell biology, have worked together to create this preprint reading lists for researchers with an interest in cell biology. Categories include: 1) cancer cell biology 2) cell cycle and division 3) cell migration and cytoskeleton 4) cell organelles and organisation 5) cell signalling and mechanosensing 6) genetics/gene expression

 



List by Felipe Del Valle Batalla et al.

BSCB-Biochemical Society 2024 Cell Migration meeting

This preList features preprints that were discussed and presented during the BSCB-Biochemical Society 2024 Cell Migration meeting in Birmingham, UK in April 2024. Kindly put together by Sara Morais da Silva, Reviews Editor at Journal of Cell Science.

 



List by Reinier Prosee

‘In preprints’ from Development 2022-2023

A list of the preprints featured in Development's 'In preprints' articles between 2022-2023

 



List by Alex Eve, Katherine Brown

preLights peer support – preprints of interest

This is a preprint repository to organise the preprints and preLights covered through the 'preLights peer support' initiative.

 



List by preLights peer support

The Society for Developmental Biology 82nd Annual Meeting

This preList is made up of the preprints discussed during the Society for Developmental Biology 82nd Annual Meeting that took place in Chicago in July 2023.

 



List by Joyce Yu, Katherine Brown

CSHL 87th Symposium: Stem Cells

Preprints mentioned by speakers at the #CSHLsymp23

 



List by Alex Eve

Journal of Cell Science meeting ‘Imaging Cell Dynamics’

This preList highlights the preprints discussed at the JCS meeting 'Imaging Cell Dynamics'. The meeting was held from 14 - 17 May 2023 in Lisbon, Portugal and was organised by Erika Holzbaur, Jennifer Lippincott-Schwartz, Rob Parton and Michael Way.

 



List by Helen Zenner

9th International Symposium on the Biology of Vertebrate Sex Determination

This preList contains preprints discussed during the 9th International Symposium on the Biology of Vertebrate Sex Determination. This conference was held in Kona, Hawaii from April 17th to 21st 2023.

 



List by Martin Estermann

Alumni picks – preLights 5th Birthday

This preList contains preprints that were picked and highlighted by preLights Alumni - an initiative that was set up to mark preLights 5th birthday. More entries will follow throughout February and March 2023.

 



List by Sergio Menchero et al.

CellBio 2022 – An ASCB/EMBO Meeting

This preLists features preprints that were discussed and presented during the CellBio 2022 meeting in Washington, DC in December 2022.

 



List by Nadja Hümpfer et al.

Fibroblasts

The advances in fibroblast biology preList explores the recent discoveries and preprints of the fibroblast world. Get ready to immerse yourself with this list created for fibroblasts aficionados and lovers, and beyond. Here, my goal is to include preprints of fibroblast biology, heterogeneity, fate, extracellular matrix, behavior, topography, single-cell atlases, spatial transcriptomics, and their matrix!

 



List by Osvaldo Contreras

EMBL Synthetic Morphogenesis: From Gene Circuits to Tissue Architecture (2021)

A list of preprints mentioned at the #EESmorphoG virtual meeting in 2021.

 



List by Alex Eve

FENS 2020

A collection of preprints presented during the virtual meeting of the Federation of European Neuroscience Societies (FENS) in 2020

 



List by Ana Dorrego-Rivas

Planar Cell Polarity – PCP

This preList contains preprints about the latest findings on Planar Cell Polarity (PCP) in various model organisms at the molecular, cellular and tissue levels.

 



List by Ana Dorrego-Rivas

BioMalPar XVI: Biology and Pathology of the Malaria Parasite

[under construction] Preprints presented at the (fully virtual) EMBL BioMalPar XVI, 17-18 May 2020 #emblmalaria

 



List by Dey Lab, Samantha Seah

1

Cell Polarity

Recent research from the field of cell polarity is summarized in this list of preprints. It comprises of studies focusing on various forms of cell polarity ranging from epithelial polarity, planar cell polarity to front-to-rear polarity.

 



List by Yamini Ravichandran

TAGC 2020

Preprints recently presented at the virtual Allied Genetics Conference, April 22-26, 2020. #TAGC20

 



List by Maiko Kitaoka et al.

3D Gastruloids

A curated list of preprints related to Gastruloids (in vitro models of early development obtained by 3D aggregation of embryonic cells). Updated until July 2021.

 



List by Paul Gerald L. Sanchez and Stefano Vianello

ECFG15 – Fungal biology

Preprints presented at 15th European Conference on Fungal Genetics 17-20 February 2020 Rome

 



List by Hiral Shah

ASCB EMBO Annual Meeting 2019

A collection of preprints presented at the 2019 ASCB EMBO Meeting in Washington, DC (December 7-11)

 



List by Madhuja Samaddar et al.

EMBL Seeing is Believing – Imaging the Molecular Processes of Life

Preprints discussed at the 2019 edition of Seeing is Believing, at EMBL Heidelberg from the 9th-12th October 2019

 



List by Dey Lab

Autophagy

Preprints on autophagy and lysosomal degradation and its role in neurodegeneration and disease. Includes molecular mechanisms, upstream signalling and regulation as well as studies on pharmaceutical interventions to upregulate the process.

 



List by Sandra Malmgren Hill

Lung Disease and Regeneration

This preprint list compiles highlights from the field of lung biology.

 



List by Rob Hynds

Cellular metabolism

A curated list of preprints related to cellular metabolism at Biorxiv by Pablo Ranea Robles from the Prelights community. Special interest on lipid metabolism, peroxisomes and mitochondria.

 



List by Pablo Ranea Robles

BSCB/BSDB Annual Meeting 2019

Preprints presented at the BSCB/BSDB Annual Meeting 2019

 



List by Dey Lab

MitoList

This list of preprints is focused on work expanding our knowledge on mitochondria in any organism, tissue or cell type, from the normal biology to the pathology.

 



List by Sandra Franco Iborra

ASCB/EMBO Annual Meeting 2018

This list relates to preprints that were discussed at the recent ASCB conference.

 



List by Dey Lab, Amanda Haage
Close