Rab11b-mediated integrin recycling promotes brain metastatic adaptation and outgrowth
Posted on: 19 July 2019
Preprint posted on 11 June 2019
Article now published in Nature Communications at http://dx.doi.org/10.1038/s41467-020-16832-2
How do breast cancer cells adapt to the brain microenvironment during metastasis? This recent preprint provides new insights into the role of Rab11b-mediated endosomal recycling.
Selected by Beverley Wilson
Categories: biochemistry, cancer biology, cell biology
Background:
Incidence of breast cancer brain metastases is increasing (Kotecki et al. 2018) and is seen as an urgent clinical problem. Brain metastases are hard to treat using standard chemotherapeutic agents, as many of these are unable to cross the blood-brain barrier, leading to an urgent need for therapeutic advance.
Disseminated tumour cells from the original primary breast tumour are known to persist in the brain for long periods before metastatic disease becomes apparent (Harper et al. 2016; Hosseini et al. 2016). Adaptation to the brain by these disseminated cells requires their engagement with the brain microenvironment, which differs vastly from that of the primary tumour.
The interaction of tumour cells with the brain microenvironment is mainly mediated by proteins on the cell surface, including a range of cell matrix adhesion receptors such as integrins. It is increasingly being shown that control of the cell surface proteome through vesicular trafficking can play a key role in regulating cancer progression, including cancer cell migration and metastasis (Caswell et al. 2008; Ye et al. 2016). Members of the RabGTPase family are key regulators of vesicular trafficking and therefore of the cell surface proteome.
The role of endosomal trafficking in regulating the cell surface proteome of disseminated breast cancer cells in the brain metastatic environment and the impact of this on their adaptation to this environment is poorly understood. Howe et al. investigate this in detail and draw conclusions which could impact the clinical approaches to treating breast cancer brain metastases.
Key findings:
Initially, the authors observed temporal transcriptional changes during breast cancer brain metastases development by RNA sequencing of both early colonising cells in the brain and late-stage brain metastases. They found extensive transcriptional reprogramming upon metastatic adaptation, with 108 genes significantly upregulated in overt brain metastases. In order to identify which of these were able to functionally drive brain metastasis the authors used a Drosophila melanogaster tumour model, in which tumours develop in the eye disc and progressively invade the adjacent brain tissue. This led to the identification of a variety of genes which promote tumour growth and spread.
One of these, Rab11b, was also shown to be increased 15-fold in brain metastases when compared to matched breast cancer cell lines, while no change in expression was observed for the other Rab11-family members Rab11a or Rab25 (Rab11c). This suggests that the brain microenvironment specifically influences Rab11b expression, indicating that Rab11b could facilitate brain metastasis.
The authors demonstrated that Rab11b plays a causal role in breast cancer brain metastasis as depletion of Rab11b is sufficient to decrease brain metastasis in a mouse model. From this the authors hypothesised that the upregulation of Rab11b in disseminated cancer cells during early adaptation to the brain microenvironment could provide a mechanism for disseminated tumour cells to regulate their cell surface proteome allowing survival.
To identify the subset of surface proteins which are dependent on Rab11b for recycling, the authors carried out mass spectrometry analysis of the cell surface proteome of Rab11b-depleted cells. Rab11b-depleted cells showed a dramatic reduction in the level of adhesion proteins present on their surface. One of these, integrin β1, was shown to be strongly expressed in brain metastases and the authors showed that Rab11b-mediated control of integrin β1 cell surface levels enables successful interaction with the brain extracellular matrix and facilitates tumour cell survival.
Finally, the authors sought a therapeutic strategy to target Rab11b. They did this by using the statins, pitavastatin and simvastatin, which inhibit the mevalonate pathway, thereby preventing the geranylgeranylation of RabGTPases leaving them unable to be localised to membranes and therefore non-functional. The use of these statins inhibited the proliferation of breast cancer cells on decellularized brain matrix and Rab11b-mediated recycling of integrin β1. The statins also dramatically reduced brain metastasis incidence in in vivo mouse models, and led to an increase in survival.
Together these experiments showed the importance of endosomal recycling by a less well studied RabGTPase, Rab11b, in the adaptation of breast cancer cells to the challenging brain metastatic microenvironment, allowing eventual proliferation (Figure 1). The authors also provide a strong rationale for the repurposing of statins to prevent breast cancer brain metastasis, indicating that this could be a clinically practical strategy.
Figure 1: Abstract figure from the preprint. Rab11b up-regulation in the brain microenvironment promotes the recycling of cargo proteins required for breast cancer brain metastasis. Inhibition of the mevalonate pathway with statins prevents geranylgeranylation of Rab11b, decreasing cargo recycling, and inhibiting brain metastasis.
Why I think this work is important:
Regulation of the cell surface proteome represents an important mechanism by which cancer progression is driven. It is known that the dependence on different RabGTPase recycling pathways varies greatly in different environments, including between 2D and 3D environments. Therefore, I like how the authors of this study made use of a variety of applicable models, from soft agar, to decellularized brain matrix and in vivo mouse models.
I’m particularly impressed that this work contains the development of the authors’ hypothesis from the initial identification of the mechanism all the way through to potential therapeutic strategies. The in vivo work using statins to reduce brain metastatic incidence shows the potential for using these in the chemoprevention of breast cancer brain metastasis, giving opportunity for driving much needed therapeutic advance.
Questions for the authors:
- As other cancer types which metastasise to the brain will be adapting to the same niche, is it likely that this mechanism of adaption of breast cancer cells to the brain microenvironment could be common to other cancer types?
- Rab11b is known to be expressed in non-epithelial tissues, including brain tissue. Could there be a link between this and why breast cancer cells specifically upregulate Rab11b and not other Rab11 family subtypes?
Bibliography:
Caswell, P.T., Chan, M., Lindsay, A.J., McCaffrey, M.W., Boettiger, D., and Norman, J.C. (2008). Rab- coupling protein coordinates recycling of α5β1 integrin and EGFR1 to promote cell migration in 3D microenvironments. J. Cell Biol. 183(1):143-155.
Harper, K.L., Sosa, M.S., Entenberg, D., Hosseini, H., Cheung, J.F., Nobre, R., et al. (2016). Mechanism of early dissemination and metastasis in Her2+ mammary cancer. Nature 540(7634):588-592
Hosseini, H., Obradović, M.M.S., Hoffmann, M., Harper, K.L., Sosa, M.S., Werner-Klein, M., et al. (2016). Early dissemination seeds metastasis in breast cancer. Nature 540(7634):552-558
Kotecki, N., Lefranc, F., Devriendt, D., and Awada, A. (2018). Therapy of breast cancer brain metastas es: challenges, emerging treatments and perspectives. Ther Adv Med Oncol. 10:1758835918780312
Ye, Q.H., Zhu, W.W., Zhang, J.B., Qin, Y., Lu, M., Lin, G.L., et al. (2016). GOLM1 modulates EGFR/RTK cellsurface recycling to drive hepatocellular carcinoma metastasis. Cancer Cell 30(3):444-458
doi: https://doi.org/10.1242/prelights.12279
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