A Transcriptional Switch Governing Fibroblast Plasticity Underlies Reversibility of Chronic Heart Disease
Preprint posted on July 22, 2020 https://www.biorxiv.org/content/10.1101/2020.07.21.214874v1
Fibroblasts: an enigmatic, diverse and plastic cell type that can determine the fate of heart failure
Selected by Osvaldo Contreras, Alexander WardBackground
Why mammalian cardiac regeneration and repair do not occur in adults remains a mystery.
Heart failure (HF) is a leading cause of mortality and hospitalization thus representing a burden to the life of patients, and the societal costs are astounding. Although progress has been made to reduce mortality and improve the quality of life of patients after HF, the 5-year mortality after an initial diagnosis of HF is about 40%. No effective therapeutics are available for the treatment of HF. Remarkably, HF worsens heart degeneration and organ dysfunction leading to progressive changes in gene expression that dynamically influence HF onset and development. HF is associated with exacerbated deposition of extracellular matrix (ECM) components and proliferation of endogenous cardiac fibroblasts that later differentiate into fibrosis-causing myofibroblasts. Among the several cell types that participate in heart repair, resident fibroblasts play a critical role as progenitor cells and by synthesizing the ECM of the heart. Thus, fibroblasts are essential in modelling and remodelling the structural integrity of tissues and organs in health and disease. Cardiac resident fibroblasts are not produced de novo and they exhibit high heterogeneity, especially after injury. This fuels the need to develop novel pharmacological approaches to combat fibrosis in HF aiming to boost heart function.
Previous preclinical work led by the authors unveiled the participation of Bromodomain and extraterminal domain (BET) protein inhibitors in attenuating heart failure. BET proteins recognise acetyl-lysine on histones and regulate gene transcription and chromatin remodelling. Hence, BETs have been targeted in disease with several ongoing clinical trials seeking to inhibit BETs in diverse pathological settings like cancer and inflammation. The authors have demonstrated that the small molecule BET inhibitor JQ1 prevents the development of cardiac hypertrophy, left ventricle dysfunction and fibrosis when administered at the onset of HF. Furthermore, JQ1 reduces pathology and improves cardiac function in mouse models of HF, even if administered after the disease is established. These promising treatments of HF via BET bromodomain inhibition seem to be mediated by JQ1 blocking inflammatory and profibrotic gene programs like TGF-b signalling. However, the precise cellular and molecular dynamics of the reparative response after HF and BET inhibitor treatment remains an unresolved puzzle.
Researchers have long suspected that endogenous fibroblasts can convert to activated states, such as myofibroblast-like, a contractile and secretory cell phenotype that actively modulates tissue scarring. Hence, both the cellular plasticity and heterogeneity exhibited by fibroblasts are of particular interest given their requirements for wound healing and regeneration. However, the capacity of activated fibroblasts and myofibroblasts to revert to a naïve or basal fibroblast state after an injury is yet unclear. In this preprint, the authors combined single-cell RNA sequencing (scRNA-seq) with single-cell Assay for Transposase-Accessible Chromatin sequencing (scATAC-seq) in a clinically-relevant adult mouse model of HF to interrogate the reversibility of fibroblast-myofibroblast cell states using BET inhibition.
Key findings
Using a classical measure of cardiac function, the Ejection Fraction, the authors first showed that the beneficial effects of BET inhibition, with JQ1 and another small molecule inhibitor, CPI-456, were reversible in the context of two independent cardiac Heart Failure (HF) models, Myocardial Infarction (MI) and Transverse Aortic Constriction (TAC). The implications of a therapeutic reversal of heart failure at later stages are enormous, given that many patients often progress to pre-HF without recognised signs or symptoms. This reversibility effect led them to ponder whether cardiac cell-states were changing with BET inhibition. Typically, during injury-induced remodelling, cell-state changes are common and can be attributed to dynamic regulatory changes of a multitude of chromatin interactors and up- and down-stream effectors. In order to assess cell-states and sub-populations of the heart, the authors separated different cellular compartments through an elegant method of ex vivo Langendorff (rig-based) perfusion, followed by size and mass separation of tissue- and cell-compartments.
Owing to the improved functional outcome, they first evaluated bulk transcriptomic changes in isolated Cardiomyocytes (CMs), the functional units of the beating heart, which surprisingly showed only very minor changes, suggesting that the functional benefit of JQ1 was affecting the non-CM compartment. In this interstitial compartment, fibroblasts and endothelial cells make up the majority of cell-types, with myeloid, endo- and epi-cardial cells making up smaller subpopulations. Using scRNA-seq, the authors profiled these non-CM subpopulations isolated from 4 separate conditions: Sham control (state 1), TAC untreated (2), TAC with constant JQ1 treatment (3) and TAC with JQ1 treatment withdrawn (4), which represented a disease regression-type model. The effect of constant JQ1 was apparent in most non-CM cell-types, with these subpopulations showing distinct clustering from other conditions. However, the pattern of the clustering within fibroblasts revealed a truly dynamic pattern of cell-state shifts, which was specific only to fibroblasts. This dynamic pattern showed that BET inhibition in fibroblasts after injury (state 3) led to the reversion back to a more quiescent and basal state, seen in the control condition (state 1). On the other hand, the withdrawal of treatment resulted in the deterioration of fibroblasts back to a TAC-like stressed state (state 2 and 4), similar to an activated, pathogenic myofibroblast. This bi-directional plasticity of cell-state correlated highly with the reversibility of cardiac function with BET inhibition, which the authors suggest is indicative of a direct influence of fibroblast state on heart failure pathogenesis.
Finally, having identified the sub-population of cardiac cells that were in part responsible for this remarkable therapeutic reversibility, the authors then sought to identify the key switch regulating fibroblast plasticity during BET inhibition. To do this at the individual cell level, authors profiled accessible chromatin and enhancer activation marks through the integration of their scRNA-seq analysis with scATAC-seq, from the same hearts. In doing this, they were able to uncover several key features of BET-dependent cell fate-switching in HF. Firstly, the transcription factor, MEOX1, was critical to the control of fibroblast plasticity during HF-induced remodelling, by directing these cells towards a pathogenic myofibroblast state. Secondly, that specific enhancer accessibility in fibroblasts could be directly correlated with positive or negative functional outcomes. And lastly, when a handful of these key enhancer regions were silenced, fibroblasts were prevented from developing characteristics typical of myofibroblasts upon challenge. All in all, this preprint represents a leap forward in our understanding of both cell-fate plasticity during the remodelling process and identifies a critical mechanism by which BET inhibition may be positively influencing heart function after injury or stress.
“To be, or not to be a myofibroblast, that is the question”.

What we liked of this preprint
This paper deserves praise by its technical flair and elegant, detailed analyses, which makes a simple, but powerful message from a complex set of data. The authors push the limits of what can be achieved from just a handful of hearts and, in doing so, reveal fundamental concepts about regeneration and cell behaviour. At a technological level, this study exemplifies how single-cell approaches can be intricately used to profile disease states and pinpoint therapeutic mechanisms. In providing a specific mechanistic link between transcriptional regulatory proteins and cell-fate-switching in disease, they may have also opened up the possibility for more targeted therapeutic approaches in the future. The implications of reversal of cell fate from a stressed/diseased-state, back to a normal/basal state, are profound, as this has long been considered the “holy grail” for regenerative biologists (or at least a part of the grail!) and this type of study certainly represents a step in the right direction in the quest of achieving true heart regeneration in adult mammals.
Future directions and questions to the authors
- Do you measure other parameters indicative of heart function during JQ1 reversibility treatment rather than ejection fraction of the left ventricle?
- We are curious about the single-cell imaging experiments concerning MEOX1 expression in the fibroblast population. Is there any evidence about the protein expression of MEOX1 in myofibroblasts after heart failure? Do MEOX1+ cells localise around interstitial fibrotic- or perivascular fibrotic-regions, or both?
- Do you expect to have similar single-cell results using the BET inhibitor CPI-456 compared to JQ1, which seems more therapeutically promising due to its high potency and better pharmacokinetic properties than JQ1?
- The work is lacking lineage tracing strategies which are helpful to understand clonality and lineage determination of cells. From what population of endogenous fibroblasts are MEOX1 expressing myofibroblasts descending from?
- Do you expect the reversibility of the dynamic myofibroblast transcriptome under stress after JQ1 treatment to be mirrored by that of the proteome and metabolome within myofibroblasts?
- Does MEOX1 play a role as a chromatin modifier in response to injury-induced signalling (e.g. after TGF-b exposure) and do you have any direct evidence of target gene changes upon challenge?
References
Role of BET protein inhibition in heart failure:
- Anand, P., Brown, J. D., Lin, C. Y., Qi, J., Zhang, R., Artero, P. C., … Haldar, S. M. (2013). BET Bromodomains Mediate Transcriptional Pause Release in Heart Failure. Cell, 154(3), 569–582. https://doi.org/10.1016/j.cell.2013.07.013
- Spiltoir, J. I., Stratton, M. S., Cavasin, M. A., Demos-Davies, K., Reid, B. G., Qi, J., … McKinsey, T. A. (2013). BET acetyl-lysine binding proteins control pathological cardiac hypertrophy. Journal of Molecular and Cellular Cardiology, 63, 175–179. https://doi.org/https://doi.org/10.1016/j.yjmcc.2013.07.017
- Duan, Q., McMahon, S., Anand, P., Shah, H., Thomas, S., Salunga, H. T., … Haldar, S. M. (2017). BET bromodomain inhibition suppresses innate inflammatory and profibrotic transcriptional networks in heart failure. Science Translational Medicine, 9(390), eaah5084. https://doi.org/10.1126/scitranslmed.aah5084
- Stratton, M. S., Bagchi, R. A., Felisbino, M. B., Hirsch, R. A., Smith, H. E., Riching, S. A., … McKinsey, T. A. (2019). Dynamic Chromatin Targeting of BRD4 Stimulates Cardiac Fibroblast Activation. Circulation Research, 125(7), 662–677. https://doi.org/10.1161/CIRCRESAHA.119.315125
- Alexanian, M., Przytycki, P. F., Micheletti, R., Padmanabhan, A., Ye, L., Travers, J. G., … Srivastava, D. (2020). A Transcriptional Switch Governing Fibroblast Plasticity Underlies Reversibility of Chronic Heart Disease. BioRxiv, 2020.07.21.214874. https://doi.org/10.1101/2020.07.21.214874
- Wang, C. Y., & Filippakopoulos, P. (2015). Beating the odds: BETs in disease. Trends in biochemical sciences, 40(8), 468–479. https://doi.org/10.1016/j.tibs.2015.06.002
Cardiac fibroblast heterogeneity and plasticity under stress and disease:
- Farbehi, N., Patrick, R., Dorison, A., Xaymardan, M., Janbandhu, V., Wystub- Lis, K., Ho, J. W. K., Nordon, R. E. and Harvey, R. P. (2019). Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury. eLife 8, e43882. doi:10.7554/eLife.43882
- Soliman, H., Paylor, B., Scott, R. W., Lemos, D. R., Chang, C., Arostegui, M., Low, M., Lee, C., Fiore, D., Braghetta, P. et al. (2020). Pathogenic potential of Hic1-expressing cardiac stromal progenitors. Cell Stem Cell 26, 205-220.e8. doi:10.1016/j.stem.2019.12.008
- Fu, X., Khalil, H., Kanisicak, O., Boyer, J. G., Vagnozzi, R. J., Maliken, B. D., Sargent, M. A., Prasad, V., Valiente-Alandi, I., Blaxall, B. C. et al. (2018). Specialized fibroblast differentiated states underlie scar formation in the infarcted mouse heart. Clin. Invest. 128, 2127-2143. doi:10.1172/JCI98215
- Contreras, O., Cruz-Soca, M., Theret, M., Soliman, H., Tung, L. W., Groppa, E., Rossi, F. M. and Brandan, E. (2019). Cross-talk between TGF-β and PDGFRα signaling pathways regulates the fate of stromal fibro–adipogenic progenitors. J. Cell Sci. 132, 232157. doi:10.1242/jcs.232157
- Forte, E., Skelly, D. A., Chen, M., Daigle, S., Morelli, K. A., Hon, O., … Furtado, M. B. (2020). Dynamic Interstitial Cell Response during Myocardial Infarction Predicts Resilience to Rupture in Genetically Diverse Mice. Cell Reports, 30(9), 3149-3163.e6. https://doi.org/https://doi.org/10.1016/j.celrep.2020.02.008
- Kanisicak, O., Khalil, H., Ivey, M. J., Karch, J., Maliken, B. D., Correll, R. N., Brody, M. J., Lin, S.-C., Aronow, B. J., Tallquist, M. D. et al. (2016). Genetic lineage tracing defines myofibroblast origin and function in the injured heart. Nat. Commun. 7, 12260. doi:10.1038/ncomms12260
- McLellan, M. A., Skelly, D. A., Dona, M. S.I., Squiers, G. T., Farrugia, G. E., Gaynor, T. L., Cohen, C. D., Pandey, R., Diep, H., Vinh, A., Rosenthal, N. A., and Pinto, A. R. (2020). High-Resolution Transcriptomic Profiling of the Heart During Chronic Stress Reveals Cellular Drivers of Cardiac Fibrosis and Hypertrophy. Circulation, 0(0). https://doi.org/10.1161/CIRCULATIONAHA.119.045115
Posted on: 11th August 2020 , updated on: 12th August 2020
doi: https://doi.org/10.1242/prelights.23879
Read preprintSign up to customise the site to your preferences and to receive alerts
Register hereAlso in the biochemistry category:
Also in the bioinformatics category:
Dissecting Mammalian Spermatogenesis Using Spatial Transcriptomics
Selected by | Martin Estermann |
The hourglass model of evolutionary conservation during embryogenesis extends to developmental enhancers with signatures of positive selection
Selected by | Irepan Salvador-Martinez |
1
The mutational landscape of human somatic and germline cells
Selected by | Kerryn Elliott |
Also in the cell biology category:
Also in the clinical trials category:
Bromodomain Inhibition Blocks Inflammation-Induced Cardiac Dysfunction and SARS-CoV2 Infection in Pre-Clinical Models
Selected by | Alexander Ward, Osvaldo Contreras |
1
Decoding distinctive features of plasma extracellular vesicles in amyotrophic lateral sclerosis
Selected by | Kristina Kuhbandner |
1
A Transcriptional Switch Governing Fibroblast Plasticity Underlies Reversibility of Chronic Heart Disease
Selected by | Osvaldo Contreras, Alexander Ward |
Also in the developmental biology category:
Cell patterning by secretion-induced plasma membrane flows
Selected by | Helen Zenner |
1
Epigenetic control of coordinated hematopoietic and cardiovascular development by Rnf2 in zebrafish
Selected by | Tim Petzold |
1
Dissecting Mammalian Spermatogenesis Using Spatial Transcriptomics
Selected by | Martin Estermann |
Also in the genomics category:
EccDNA formation is dependent on MMEJ, repressed by c-NHEJ pathway, and stimulated by DNA double-strand break
Selected by | Ram |
1
Association analysis of repetitive elements and R-loop formation across species
Selected by | Ram |
1
The impact of biological sex on alternative splicing
Selected by | Jennifer Ann Black |
Also in the immunology category:
Dissecting Mammalian Spermatogenesis Using Spatial Transcriptomics
Selected by | Martin Estermann |
Integrative Brain Transcriptome Analysis Links Complement Component 4 and HSPA2 to the APOE ε2 Protective Effect in Alzheimer Disease
Selected by | Theresa Pohlkamp |
Sick Bats Stay Home Alone: Social distancing during the acute phase response in Egyptian fruit bats (Rousettus aegyptiacus)
Selected by | Baheerathan Murugavel |
Also in the pathology category:
Bacteriological and histopathological findings in cetaceans that stranded in the Philippines from 2017 to 2018
Selected by | Paul Gerald L. Sanchez and Stefano Vianello |
1
Adaptive cell invasion maintains organ homeostasis
Selected by | Mariana De Niz |
1
Endothelial cell invasiveness is controlled by myosin IIA-dependent inhibition of Arp2/3 activity
Selected by | Mariana De Niz |
1
Also in the physiology category:
Intraspecific variation in thermal tolerance differs between tropical and temperate fishes
Selected by | Charlotte Nelson |
Dissecting Mammalian Spermatogenesis Using Spatial Transcriptomics
Selected by | Martin Estermann |
Sick Bats Stay Home Alone: Social distancing during the acute phase response in Egyptian fruit bats (Rousettus aegyptiacus)
Selected by | Baheerathan Murugavel |
Also in the systems biology category:
Downstream of gasdermin D cleavage, a Ragulator-Rag-mTORC1 pathway promotes pore formation and pyroptosis
Selected by | Connor Rosen |
Bromodomain Inhibition Blocks Inflammation-Induced Cardiac Dysfunction and SARS-CoV2 Infection in Pre-Clinical Models
Selected by | Alexander Ward, Osvaldo Contreras |
1
Generative modeling of single-cell population time series for inferring cell differentiation landscapes
Selected by | Yen-Chung Chen |
preListsbiochemistry category:
in theASCB EMBO Annual Meeting 2019
A collection of preprints presented at the 2019 ASCB EMBO Meeting in Washington, DC (December 7-11)
List by | Madhuja Samaddar, Ramona Jühlen, Amanda Haage, Laura McCormick, Maiko Kitaoka |
EMBL Seeing is Believing – Imaging the Molecular Processes of Life
Preprints discussed at the 2019 edition of Seeing is Believing, at EMBL Heidelberg from the 9th-12th October 2019
List by | Dey Lab |
Cellular metabolism
A curated list of preprints related to cellular metabolism at Biorxiv by Pablo Ranea Robles from the Prelights community. Special interest on lipid metabolism, peroxisomes and mitochondria.
List by | Pablo Ranea Robles |
MitoList
This list of preprints is focused on work expanding our knowledge on mitochondria in any organism, tissue or cell type, from the normal biology to the pathology.
List by | Sandra Franco Iborra |
Also in the bioinformatics category:
Single Cell Biology 2020
A list of preprints mentioned at the Wellcome Genome Campus Single Cell Biology 2020 meeting.
List by | Alex Eve |
Antimicrobials: Discovery, clinical use, and development of resistance
Preprints that describe the discovery of new antimicrobials and any improvements made regarding their clinical use. Includes preprints that detail the factors affecting antimicrobial selection and the development of antimicrobial resistance.
List by | Zhang-He Goh |
Also in the cell biology category:
FENS 2020
A collection of preprints presented during the virtual meeting of the Federation of European Neuroscience Societies (FENS) in 2020
List by | Ana Dorrego-Rivas |
Planar Cell Polarity – PCP
This preList contains preprints about the latest findings on Planar Cell Polarity (PCP) in various model organisms at the molecular, cellular and tissue levels.
List by | Ana Dorrego-Rivas |
BioMalPar XVI: Biology and Pathology of the Malaria Parasite
[under construction] Preprints presented at the (fully virtual) EMBL BioMalPar XVI, 17-18 May 2020 #emblmalaria
List by | Dey Lab, Samantha Seah |
1
Cell Polarity
Recent research from the field of cell polarity is summarized in this list of preprints. It comprises of studies focusing on various forms of cell polarity ranging from epithelial polarity, planar cell polarity to front-to-rear polarity.
List by | Yamini Ravichandran |
TAGC 2020
Preprints recently presented at the virtual Allied Genetics Conference, April 22-26, 2020. #TAGC20
List by | Maiko Kitaoka, Madhuja Samaddar, Miguel V. Almeida, Sejal Davla, Jennifer Ann Black, Dey Lab |
3D Gastruloids
A curated list of preprints related to Gastruloids (in vitro models of early development obtained by 3D aggregation of embryonic cells). Preprint missing? Don't hesitate to let us know.
List by | Paul Gerald L. Sanchez and Stefano Vianello |
ECFG15 – Fungal biology
Preprints presented at 15th European Conference on Fungal Genetics 17-20 February 2020 Rome
List by | Hiral Shah |
ASCB EMBO Annual Meeting 2019
A collection of preprints presented at the 2019 ASCB EMBO Meeting in Washington, DC (December 7-11)
List by | Madhuja Samaddar, Ramona Jühlen, Amanda Haage, Laura McCormick, Maiko Kitaoka |
EMBL Seeing is Believing – Imaging the Molecular Processes of Life
Preprints discussed at the 2019 edition of Seeing is Believing, at EMBL Heidelberg from the 9th-12th October 2019
List by | Dey Lab |
Autophagy
Preprints on autophagy and lysosomal degradation and its role in neurodegeneration and disease. Includes molecular mechanisms, upstream signalling and regulation as well as studies on pharmaceutical interventions to upregulate the process.
List by | Sandra Malmgren Hill |
Lung Disease and Regeneration
This preprint list compiles highlights from the field of lung biology.
List by | Rob Hynds |
Cellular metabolism
A curated list of preprints related to cellular metabolism at Biorxiv by Pablo Ranea Robles from the Prelights community. Special interest on lipid metabolism, peroxisomes and mitochondria.
List by | Pablo Ranea Robles |
BSCB/BSDB Annual Meeting 2019
Preprints presented at the BSCB/BSDB Annual Meeting 2019
List by | Dey Lab |
MitoList
This list of preprints is focused on work expanding our knowledge on mitochondria in any organism, tissue or cell type, from the normal biology to the pathology.
List by | Sandra Franco Iborra |
Biophysical Society Annual Meeting 2019
Few of the preprints that were discussed in the recent BPS annual meeting at Baltimore, USA
List by | Joseph Jose Thottacherry |
ASCB/EMBO Annual Meeting 2018
This list relates to preprints that were discussed at the recent ASCB conference.
List by | Dey Lab, Amanda Haage |
Also in the clinical trials category:
Autophagy
Preprints on autophagy and lysosomal degradation and its role in neurodegeneration and disease. Includes molecular mechanisms, upstream signalling and regulation as well as studies on pharmaceutical interventions to upregulate the process.
List by | Sandra Malmgren Hill |
Antimicrobials: Discovery, clinical use, and development of resistance
Preprints that describe the discovery of new antimicrobials and any improvements made regarding their clinical use. Includes preprints that detail the factors affecting antimicrobial selection and the development of antimicrobial resistance.
List by | Zhang-He Goh |
Also in the developmental biology category:
EMBL Conference: From functional genomics to systems biology
Preprints presented at the virtual EMBL conference "from functional genomics and systems biology", 16-19 November 2020
List by | Jesus Victorino |
Single Cell Biology 2020
A list of preprints mentioned at the Wellcome Genome Campus Single Cell Biology 2020 meeting.
List by | Alex Eve |
Society for Developmental Biology 79th Annual Meeting
Preprints at SDB 2020
List by | Irepan Salvador-Martinez, Martin Estermann |
FENS 2020
A collection of preprints presented during the virtual meeting of the Federation of European Neuroscience Societies (FENS) in 2020
List by | Ana Dorrego-Rivas |
Planar Cell Polarity – PCP
This preList contains preprints about the latest findings on Planar Cell Polarity (PCP) in various model organisms at the molecular, cellular and tissue levels.
List by | Ana Dorrego-Rivas |
Cell Polarity
Recent research from the field of cell polarity is summarized in this list of preprints. It comprises of studies focusing on various forms of cell polarity ranging from epithelial polarity, planar cell polarity to front-to-rear polarity.
List by | Yamini Ravichandran |
TAGC 2020
Preprints recently presented at the virtual Allied Genetics Conference, April 22-26, 2020. #TAGC20
List by | Maiko Kitaoka, Madhuja Samaddar, Miguel V. Almeida, Sejal Davla, Jennifer Ann Black, Dey Lab |
3D Gastruloids
A curated list of preprints related to Gastruloids (in vitro models of early development obtained by 3D aggregation of embryonic cells). Preprint missing? Don't hesitate to let us know.
List by | Paul Gerald L. Sanchez and Stefano Vianello |
ASCB EMBO Annual Meeting 2019
A collection of preprints presented at the 2019 ASCB EMBO Meeting in Washington, DC (December 7-11)
List by | Madhuja Samaddar, Ramona Jühlen, Amanda Haage, Laura McCormick, Maiko Kitaoka |
EDBC Alicante 2019
Preprints presented at the European Developmental Biology Congress (EDBC) in Alicante, October 23-26 2019.
List by | Sergio Menchero, Jesus Victorino, Teresa Rayon, Irepan Salvador-Martinez |
EMBL Seeing is Believing – Imaging the Molecular Processes of Life
Preprints discussed at the 2019 edition of Seeing is Believing, at EMBL Heidelberg from the 9th-12th October 2019
List by | Dey Lab |
SDB 78th Annual Meeting 2019
A curation of the preprints presented at the SDB meeting in Boston, July 26-30 2019. The preList will be updated throughout the duration of the meeting.
List by | Alex Eve |
Lung Disease and Regeneration
This preprint list compiles highlights from the field of lung biology.
List by | Rob Hynds |
Young Embryologist Network Conference 2019
Preprints presented at the Young Embryologist Network 2019 conference, 13 May, The Francis Crick Institute, London
List by | Alex Eve |
Pattern formation during development
The aim of this preList is to integrate results about the mechanisms that govern patterning during development, from genes implicated in the processes to theoritical models of pattern formation in nature.
List by | Alexa Sadier |
BSCB/BSDB Annual Meeting 2019
Preprints presented at the BSCB/BSDB Annual Meeting 2019
List by | Dey Lab |
Zebrafish immunology
A compilation of cutting-edge research that uses the zebrafish as a model system to elucidate novel immunological mechanisms in health and disease.
List by | Shikha Nayar |
Also in the genomics category:
EMBL Conference: From functional genomics to systems biology
Preprints presented at the virtual EMBL conference "from functional genomics and systems biology", 16-19 November 2020
List by | Jesus Victorino |
TAGC 2020
Preprints recently presented at the virtual Allied Genetics Conference, April 22-26, 2020. #TAGC20
List by | Maiko Kitaoka, Madhuja Samaddar, Miguel V. Almeida, Sejal Davla, Jennifer Ann Black, Dey Lab |
Also in the immunology category:
Single Cell Biology 2020
A list of preprints mentioned at the Wellcome Genome Campus Single Cell Biology 2020 meeting.
List by | Alex Eve |
Autophagy
Preprints on autophagy and lysosomal degradation and its role in neurodegeneration and disease. Includes molecular mechanisms, upstream signalling and regulation as well as studies on pharmaceutical interventions to upregulate the process.
List by | Sandra Malmgren Hill |
Antimicrobials: Discovery, clinical use, and development of resistance
Preprints that describe the discovery of new antimicrobials and any improvements made regarding their clinical use. Includes preprints that detail the factors affecting antimicrobial selection and the development of antimicrobial resistance.
List by | Zhang-He Goh |
Zebrafish immunology
A compilation of cutting-edge research that uses the zebrafish as a model system to elucidate novel immunological mechanisms in health and disease.
List by | Shikha Nayar |
Also in the pathology category:
ECFG15 – Fungal biology
Preprints presented at 15th European Conference on Fungal Genetics 17-20 February 2020 Rome
List by | Hiral Shah |
COVID-19 / SARS-CoV-2 preprints
List of important preprints dealing with the ongoing coronavirus outbreak. See http://covidpreprints.com for additional resources and timeline, and https://connect.biorxiv.org/relate/content/181 for full list of bioRxiv and medRxiv preprints on this topic
List by | Dey Lab, Zhang-He Goh |
1
Cellular metabolism
A curated list of preprints related to cellular metabolism at Biorxiv by Pablo Ranea Robles from the Prelights community. Special interest on lipid metabolism, peroxisomes and mitochondria.
List by | Pablo Ranea Robles |
Also in the physiology category:
FENS 2020
A collection of preprints presented during the virtual meeting of the Federation of European Neuroscience Societies (FENS) in 2020
List by | Ana Dorrego-Rivas |
TAGC 2020
Preprints recently presented at the virtual Allied Genetics Conference, April 22-26, 2020. #TAGC20
List by | Maiko Kitaoka, Madhuja Samaddar, Miguel V. Almeida, Sejal Davla, Jennifer Ann Black, Dey Lab |
Autophagy
Preprints on autophagy and lysosomal degradation and its role in neurodegeneration and disease. Includes molecular mechanisms, upstream signalling and regulation as well as studies on pharmaceutical interventions to upregulate the process.
List by | Sandra Malmgren Hill |
Also in the systems biology category:
Single Cell Biology 2020
A list of preprints mentioned at the Wellcome Genome Campus Single Cell Biology 2020 meeting.
List by | Alex Eve |
ASCB EMBO Annual Meeting 2019
A collection of preprints presented at the 2019 ASCB EMBO Meeting in Washington, DC (December 7-11)
List by | Madhuja Samaddar, Ramona Jühlen, Amanda Haage, Laura McCormick, Maiko Kitaoka |
EMBL Seeing is Believing – Imaging the Molecular Processes of Life
Preprints discussed at the 2019 edition of Seeing is Believing, at EMBL Heidelberg from the 9th-12th October 2019
List by | Dey Lab |
Pattern formation during development
The aim of this preList is to integrate results about the mechanisms that govern patterning during development, from genes implicated in the processes to theoritical models of pattern formation in nature.
List by | Alexa Sadier |