Poly-ADP-ribosylation drives loss of protein homeostasis in ATM and Mre11 deficiency
Posted on: 13 November 2020 , updated on: 17 November 2020
Preprint posted on 27 October 2020
Article now published in Molecular Cell at http://dx.doi.org/10.1016/j.molcel.2021.01.019
Poly-ADP-ribosylation lies at the crossroads of oxidative stress, DNA damage, and protein homeostasis in ataxia-telangiectasia.
Selected by Sree Rama Chaitanya
Categories: biochemistry, cell biology, molecular biology, neuroscience
Context1-4
Maintaining genome stability is central to cellular homeostasis, failure of which results in several human maladies. In eukaryotes, Ataxia-Telangiectasia Mutated (ATM), a master regulator of DNA damage response, swiftly supports genome stability by orchestrating the phosphorylation of several proteins either to arrest cell cycle or repair damaged DNA. ATM facilitates DNA damage response under the key upstream regulation by the Mre11-Rad50-Nbs1 complex (MRN). ATM also thwarts oxidative stress (reactive oxygen species, ROS), that can work independently of the DNA damage response pathway. ATM loss-of-function mutations or deletion causes a plethora of neurodegenerative disorders, predisposition to cancer, and immunodeficiency in humans.
Previous studies demonstrated a causative link between protein aggregation and neurotoxicity in many neurodegenerative disorders. In fact, the host lab found that cells derived from ataxia-telangiectasia (A-T) patients manifest rampant protein aggregation exaggerated by oxidative stress. Now in the current study, they extended their earlier findings and investigate the mechanistic details of protein aggregation in ATM deficiency and how this is linked to A-T.
Key findings
- First, the authors identified aggregated proteins in ATM depleted osteosarcoma (U2OS) and glioblastoma (U87-MG) cell lines using biochemical fractionation followed by label-free mass spectrometry. They found an overlap of 84% of aggregated polypeptides between the two cell types. Furthermore, protein aggregation diminished with anti-oxidant treatment (N-acetyl cysteine, NAC).
- ATM-deficient cells accumulate poly-ADP-ribosylated (PAR) proteins5. As PARylation by poly-ADP-ribopolymerases (PARPs) coalesce intrinsically disordered proteins at the sites of DNA damage6, the authors envisaged a possibility of PARPs in mediating protein aggregation in ATM deficiency. They tested this by inhibiting PARPs using genetic manipulation (via shRNA) and pharmacological (veliparib). They found that PARP inhibition is sufficient to prevent protein aggregation of two tested proteins PSMB2 and CK2β, under ATM deficiency.
(1) PARylation sensor, (2) PBZ-CK2β PARylation system. (3) Proposed model. Taken and modified from Fig 2 and 4 of Lee JH et. al., 2020 under a CC-BY 4.0 international license. - The authors then utilized a fluorescent PAR sensor that constitutes an engineered PAR binding domain (PBZ) with a split-venus protein fluorescence detection system7 (fig.1). Using this PARylation imaging sensor, they demonstrated prominent pan-nuclear foci of PARylated proteins in cells inhibited of ATM or treated with arsenite (to induce protein aggregation) but not in NAC treated cells. They also custom engineered PBZ and CK2β domains (fig.2) and figured that PBZ-CK2β PARylated fluorescent loci pop up in the nucleus when ATM is inhibited but not in NAC treated cells. Intriguingly, they observed PBZ-CK2β PARylated foci in ATM mutant deficient in ROS-mediated activation (C2991L, CL) and kinase-dead (D2889A, DA) but not in the mutant deficient in MRN-driven pathway (2RA).
- As PARP is implicated in DNA damage response, the authors evaluated the nature of DNA breaks in ATM mutants. They found that ATM mutants deficient in ROS-pathway – CL, and R3047X (RX) – manifested higher levels of single-stranded DNA breaks that are reduced by NAC (assessed by alkaline comet assay). Note that ATM mutant RX is also found in some individuals suffering from A-T. Furthermore, DNA breaks in these mutants were reduced under transient inhibition of transcription (using 5,6-dichloro-1-β-D-ribofuranosyl benzimidazole, DRB).
- Transcription-associated DNA damage is driven by co-transcriptionally formed RNA-DNA hybrids (also called R-loops)8. So, the authors ectopically expressed RNA-DNA helicase Senataxin (SETX) that is known to resolve R-loops. They found that SETX expression reduced the DNA breaks in the ATM mutants. Moreover, NAC treatment and SETX expression reduced aberrant R-loops formed at R-loop prone gene loci (BTBD19, ACTB, EGR1) in cells expressing ATM mutants (assayed by DNA-RNA immunoprecipitation, DRIP-qPCR). Moreover, resolving R-loops (SETX) and inhibiting transcription (DRB) also reduced PSMB2 and CK2β protein aggregation and PARylation.
- Surprisingly, they also found that mutations in Mre11 (MRN complex) that cause an A-T like disorder also induced widespread protein aggregation, PARylation, and DNA breaks on par with ATM loss, albeit independent of ROS. Thus, the authors suggest that ROS-dependent ATM mutants induce transcription and R-loop associated DNA damage that triggers PARylation and protein aggregation.
- The authors then extended the above findings to individuals suffering from A-T disorder. For this purpose, they collected fresh-frozen cerebellum tissue from 21 individuals battling with A-T and 21 healthy individuals that acted as control matched to age, sex, and ethnicity. They report a core set of 189 aggregation-prone proteins when comparing the clinical and cellular data (by mass-spectrometry). The aggregation-prone protein list includes heat-shock proteins, metabolic enzymes, oxidoreductases, ion-channel transporters, ataxin-10, and spinocerebellar ataxia type 10 that support neuronal health. Notably, the A-T derived cerebellum tissues revealed prominent anti-PAR signal (assayed by immunohistochemistry).
Conclusion and perspective
In the current preprint, the authors demonstrate that ATM leads to aberrant R-loop-mediated DNA damage in a ROS-dependent pathway (fig.3). They suggest that R-loops and DNA damage can instigate widespread PARylation and protein aggregation. Loss of protein homeostasis was observed in many neurodegenerative diseases; however, the authors report dysregulated protein homeostasis for the first time in A-T (and A-T like disorder).
Aberrant R-loops and R-loop mediated DNA damage is implicated in the etiology of many neurological diseases, cause of mutations in proteins that maintain R-loop homeostasis9,10. Here the authors propose a model where ROS-mediated R-loops and DNA damage could coalesce PARylated protein aggregates that can cause neurotoxicity (at least in A-T).
Acknowledgments
Thanks to all the authors for their support, especially Tanya Paull, for taking the time to comment on the highlight and replying promptly.
References
- https://doi.org/10.1186/s13023-016-0543-7
- https://doi.org/10.1146/annurev-biochem-060614-034335
- https://doi.org/10.1126/science.1192912
- https://doi.org/10.1126/scisignal.aan5598
- https://doi.org/10.1016/j.cmet.2016.09.004
- https://doi.org/10.1038/ncomms9088
- https://doi.org/10.1038/s41467-018-04466-4
- https://doi.org/10.1016/j.cell.2019.08.055
- https://doi.org/10.1007/s12035-018-1246-y
- https://dx.doi.org/10.1016%2Fj.jmb.2016.08.031
- https://doi.org/10.1073/pnas.1611673113
- https://doi.org/10.1242/jcs.244129
doi: https://doi.org/10.1242/prelights.25680
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| List by | Sandra Franco Iborra |
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May in preprints – the CellBio edition
A group of preLighters, with expertise in different areas of cell biology, have worked together to create this preprint reading lists for researchers with an interest in cell biology. This month, categories include: 1) Biochemistry/metabolism 2) Cancer cell Biology 3) Cell adhesion, migration and cytoskeleton 4) Cell organelles and organisation 5) Cell signalling and 6) Genetics
| List by | Barbora Knotkova et al. |
April in preprints – the CellBio edition
A group of preLighters, with expertise in different areas of cell biology, have worked together to create this preprint reading lists for researchers with an interest in cell biology. This month, categories include: 1) biochemistry/metabolism 2) cell cycle and division 3) cell organelles and organisation 4) cell signalling and mechanosensing 5) (epi)genetics
| List by | Vibha SINGH et al. |
Biologists @ 100 conference preList
This preList aims to capture all preprints being discussed at the Biologists @100 conference in Liverpool, UK, either as part of the poster sessions or the (flash/short/full-length) talks.
| List by | Reinier Prosee, Jonathan Townson |
2024 Hypothalamus GRC
This 2024 Hypothalamus GRC (Gordon Research Conference) preList offers an overview of cutting-edge research focused on the hypothalamus, a critical brain region involved in regulating homeostasis, behavior, and neuroendocrine functions. The studies included cover a range of topics, including neural circuits, molecular mechanisms, and the role of the hypothalamus in health and disease. This collection highlights some of the latest advances in understanding hypothalamic function, with potential implications for treating disorders such as obesity, stress, and metabolic diseases.
| List by | Nathalie Krauth |
‘In preprints’ from Development 2022-2023
A list of the preprints featured in Development's 'In preprints' articles between 2022-2023
| List by | Alex Eve, Katherine Brown |
CSHL 87th Symposium: Stem Cells
Preprints mentioned by speakers at the #CSHLsymp23
| List by | Alex Eve |
Journal of Cell Science meeting ‘Imaging Cell Dynamics’
This preList highlights the preprints discussed at the JCS meeting 'Imaging Cell Dynamics'. The meeting was held from 14 - 17 May 2023 in Lisbon, Portugal and was organised by Erika Holzbaur, Jennifer Lippincott-Schwartz, Rob Parton and Michael Way.
| List by | Helen Zenner |
FENS 2020
A collection of preprints presented during the virtual meeting of the Federation of European Neuroscience Societies (FENS) in 2020
| List by | Ana Dorrego-Rivas |
ASCB EMBO Annual Meeting 2019
A collection of preprints presented at the 2019 ASCB EMBO Meeting in Washington, DC (December 7-11)
| List by | Madhuja Samaddar et al. |
SDB 78th Annual Meeting 2019
A curation of the preprints presented at the SDB meeting in Boston, July 26-30 2019. The preList will be updated throughout the duration of the meeting.
| List by | Alex Eve |
Autophagy
Preprints on autophagy and lysosomal degradation and its role in neurodegeneration and disease. Includes molecular mechanisms, upstream signalling and regulation as well as studies on pharmaceutical interventions to upregulate the process.
| List by | Sandra Malmgren Hill |
Young Embryologist Network Conference 2019
Preprints presented at the Young Embryologist Network 2019 conference, 13 May, The Francis Crick Institute, London
| List by | Alex Eve |