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Single Cell Analysis Reveals Immune Cell-Adipocyte Crosstalk Regulating the Transcription of Thermogenic Adipocytes

Prashant Rajbhandari, Douglas Arneson, An-Chieh Feng, In Sook Ahn, Graciel Diamante, Nima Zaghari, Brandon J. Thomas, Laurent Vergnes, Stephen D. Lee, Karen Reue, Stephen T. Smale, Xia Yang, Peter Tontonoz

Preprint posted on June 13, 2019 https://www.biorxiv.org/content/10.1101/669853v1

Article now published in eLife at http://dx.doi.org/10.7554/eLife.49501

It’s getting hot in here: single cell sequencing reveals new insights into the role of the immune system in white adipocyte beiging & thermogenesis

Selected by Matthew Sinton

Context & Background

Adipose tissue is the major site of lipid storage and is broadly divided into three distinct groups: white adipose tissue (WAT), brown adipose tissue (BAT), and bone marrow adipose tissue (BMAT). Whereas WAT functions predominantly as an energy storage depot, BAT generates heat through uncoupling of the electron transport chain, via UCP1, and futile creatine cycling1. A subpopulation of adipocytes, in particular WAT depots, also have the ability to acquire a brown-like, or beige, phenotype following cold exposure2.

With the global increase in obesity levels, there is increased interest in the mechanisms by which WAT can acquire a beige phenotype, as this may open an avenue to the development of new therapeutics for weight loss. It appears that the beiging process is tightly controlled by the immune system3–5. In particular, it is regulated by interleukin 10 (IL-10), which blocks b-adrenergic signalling and beiging, through antagonism of IL-10 receptor subunit alpha (IL10RA)6. However, it was not clear whether IL-10 is specifically targeting adipocytes or other cell types.

In this preprint, the authors demonstrate, through a combination of adipocyte-specific IL10RA knockouts and single cell sequencing, that IL-10 acts directly on adipocytes to block beiging. Furthermore, through their single cell sequencing they identify a subpopulation of thermogenic adipocytes.

 

Key findings

Loss of adipocyte IL10RA protects against obesity and promotes thermogenesis

The authors show that adipocyte-specific deletion of IL10RA decreases adipose mass and concurrently increase lean mass in mice. This was not an effect of increased activity or decreased food intake. When placed on a high fat diet (HFD), the knockout mice were resistant to development of obesity. Furthermore, upon cold exposure, or administration of a b-adrenergic agonist, IL10RA knockout mice were able to upregulate genes associated with thermogenesis.

 

Inguinal WAT contains sub-populations of thermogenic adipocytes

To identify populations of thermogenic adipocytes in inguinal WAT (iWAT), the authors developed a single nucleus adipocyte RNA sequencing (SNAP-seq) protocol to overcome the difficulties of using lipid-laden cells. Following 24 hours of cold exposure, the authors were able to identify 14 distinct clusters in iWAT, all of which contained mature adipocyte markers (including Fabp4 and Cd36). Of these clusters, one, termed Type 9, was enriched for numerous genes related to fatty acid metabolism and thermogenesis, suggesting that they may display a beige phenotype.

 

Type 9 adipocytes become thermogenic following cold exposure

Mice were cold exposed for 24, 48 and 96 hours, or received a b-adrenergic agonist administration for 4 days. The authors then used SNAP-seq to analyse the iWAT depot and, performed pathway enrichment analysis. In the Type 9 cluster, they identified pathways associated with fatty acid metabolic processes and PPAR signalling, which were not enriched in other clusters. They also identified upregulation of a number of genes, including Ucp1, Ppargc1a, and Cidea, which are signatures of bona-fide thermogenic adipocytes.

 

SNAP-seq shows that subcutaneous WAT is comprised of heterogeneous adipocyte populations, with distinct biological functions

The wide-ranging transcriptional gene signatures and pathway enrichments of the multiple clusters identified in SNAP-seq shows, in unprecedented detail, how varied the WAT adipocyte population is. It also demonstrates that IL-10 targets a distinct subpopulation of adipocytes, raising the possibility that distinct adipocyte populations have specialised functions.

 

Lymphocytes are a major source of IL-10 in the iWAT depot

The authors performed scRNA-seq of the iWAT stromal vascular fraction and found that b-adrenergic or cold stimulation resulted in upregulation of IL-10 transcripts in both B and T cell populations. This strongly suggests that these lymphocytes provide IL-10 to stimulate adipocyte IL10RA.

 

Importance of this preprint & why I chose it

This paper was of interest because it further shows how a tissue that was previously believed to be homogeneous and relatively inert is, in fact, highly dynamic and plastic. I chose it because the authors made great use of single cell sequencing technology to tease apart the cell populations residing in the iWAT depot, and to demonstrate the thermogenic response of a subset of cells.

 

Open questions

How do other WAT depots respond to IL10RA knockout, cold exposure, or adrenergic stimulation? Considering that it is predominantly the Type 9 subset of iWAT adipocytes responding to thermogenic stimulation, do you think that this might be observed in other WAT depots?

 

The pathway enrichment analyses determined that mitochondrial and oxidative phosphorylation pathways are enriched. Do you think that this translates to a change in mitochondrial respiration in these adipocytes, or even a change in mitochondrial number?

 

Do you have any indications of the biological roles of the other 13 adipocytes in iWAT?

 

References

  1. Bertholet, A. M. et al. Mitochondrial Patch Clamp of Beige Adipocytes Reveals UCP1-Positive and UCP1-Negative Cells Both Exhibiting Futile Creatine Cycling. Cell Metab. 25, 811-822.e4 (2017).
  2. Chechi, K., van Marken Lichtenbelt, W. & Richard, D. Brown and beige adipose tissues: phenotype and metabolic potential in mice and men. J. Appl. Physiol. 124, 482–496 (2017).
  3. Brestoff, J. R. et al. Group 2 innate lymphoid cells promote beiging of white adipose tissue and limit obesity. Nature 519, 242–246 (2015).
  4. Lee, M. W. et al. Activated type 2 innate lymphoid cells regulate beige fat biogenesis. Cell 160, 74–87 (2015).
  5. Fabbiano, S. et al. Caloric Restriction Leads to Browning of White Adipose Tissue through Type 2 Immune Signaling. Cell Metab. 24, 434–446 (2016).
  6. Rajbhandari, P. et al. IL-10 Signaling Remodels Adipose Chromatin Architecture to Limit Thermogenesis and Energy Expenditure. Cell 172, 218-233.e17 (2018).

Tags: adipocytes, immunometabolism, metabolism, snapseq, thermogenesis

Posted on: 5th July 2019

doi: https://doi.org/10.1242/prelights.11733

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