Close
Close

preLights is supported by…

Related posts

Related preLists

A Window into Mammalian Basement Membrane Development: Insights from the mTurq2-Col4a1 Mouse Model

Rebecca A. Jones, Brandon Trejo, Parijat Sil, Katherine A. Little,, H. Amalia Pasolli, Bradley Joyce, Eszter Posfai, Danelle Devenport

Preprint posted on 27 September 2023 https://www.biorxiv.org/content/10.1101/2023.09.27.559396v1

Illuminating Insights into basement membrane dynamics.

Selected by Uwe Töpfer

Categories: cell biology, developmental biology, genetics

Background

Basement membranes are thin, specialized extracellular matrixes surrounding most tissues and underlying the basal side of epithelia (1). These protein networks provide cells a substrate to attach as well as important biophysical and biochemical information (2). During morphogenesis, basement membranes are remodeled to allow cells and tissues to grow and change shape (3). These basement membrane dynamics are poorly understood in mammals. This is mainly due to the lack of models in which the core basement membrane components can be endogenously labeled.

The mouse skin is a suitable tissue for studying cellular and tissue behaviors. Skin explants allow easy ex vivo culturing and the use of high-resolution microscopy. The periodically invaginating hair follicles can be used to analyze basement membrane dynamics in the context of the budding of a tissue. The epidermis consists of five different layers: stratum basale, stratum spinosum, stratum granulosum, stratum lucidum, and stratum corneum. The basement membrane underlies the basal epithelium layer and faces the dermis. It contains the major basement membrane component, Collagen IV. Collagen IV is expressed exclusively in the basement membrane and is encoded by six different genes in mammals (Collagen4A1-6).

The preprint discussed here offers an in-depth exploration of basement membrane development using a novel transgenic mouse model.

Key Findings:

A novel mTurq2-Col4a1 transgenic mouse line

In contrast to invertebrate genetic model organisms, there are only a few genetic mouse lines that enable visualization of basement membrane components. This is a gap that needs to be filled in order to understand the role of the basement membrane in mammalian development. The authors introduced the mTurq2-Col4a1 mouse model which allows real-time visualization of basement membrane development. Specifically, the authors used CRISPR/Cas9 to generate a fluorescent fusion protein of Col4a1, the ubiquitously expressed collagen IV subunit. Considering the brightness and chemical properties of the mTurquoise2 fluorophore that was used, this model offers a novel way to study the dynamic changes that occur during basement membrane formation.

Validation of mTurq2-Col4a1 functionality

mTurq2-Col4a1 transgenic mice are not homozygously viable. Nevertheless, the authors provide a series of experimental results they obtained confirming the proper localization and functionality of this protein: the mTurq2-Col4a1 protein is incorporated into the basement membrane in the correct spatial distribution, colocalizes with Collagen IV and Perlecan antibody staining, and heterozygous mice show the same basement membrane thickness as control animals in transmission electron microscopy (TEM) images. Taken together, the mTurq2-Col4a1 mouse model is a suitable tool for studying basement membrane dynamics.

Figure 1: (A) Schematic illustration with mTurquoise2-tagged Collagen4a1 and Collagen 4 heterotrimer. (B) Collagen4a1 locus with insertion site of mTurquoise2. (C-E’’) Fluorescence signal of mTurq2-Col4a1 colocalizes with anti-Collagen IV and anti-Perlecan antibody staining in the adult skin.

Basement membrane dynamics

To study the dynamics of the basement membrane during development at the highest possible resolution, the authors developed a planar-sagittal multiview imaging approach. Skin explants were folded over an agarose gel to image in XY and XZ planes at the same time. This allowed the authors to perform long-term live imaging of basement membrane dynamics during the morphogenesis of hair follicles.

Unequal Collagen IV density

Previous studies have shown perforated and thinned basement membranes during budding and branching morphogenesis (4,5). Surprisingly, in this study the mTurq2-Col4a1 intensity was higher at the actively growing base of developing hair follicles than in the neighboring region.

The basement membrane is highly pliable

When epidermal cells undergo cell division, they do not detach from the basement membrane. Moreover, the basement membrane becomes deformed by the dividing rounding cells, indicating a high pliability of the basement membrane during hair follicle development.

Figure 2: (B) Schematic illustration of planar-sagittal multiview imaging. (C) Long-term live imaging of developing hair follicles (cells in red, Col4a1 in cyan). (D, E) Protein density of Col4a1 is higher in the invaginating region of developing hair follicles than in the interfollicular epidermis. (F, G) Dividing cells stay attached and deform the basement membrane.

High stability of Collagen IV

Finally, the authors could show high stability of Collagen IV using a Fluorescence Recovery After Photobleaching (FRAP) approach.

 

Importance

This preprint is a significant contribution to the field of basement membrane biology. The novel mouse model and imaging techniques used in this study provide a new perspective on basement membrane development. A functional collagen IV fusion protein will likely advance the study of basement membranes in mice to the same extent that it did in Drosophila for invertebrates. This preprint yields surprising new results. The key findings that Collagen IV protein density is increased during hair follicle invagination, that the basement membrane is highly pliable and that cells maintain adhesion even during cell division are important new insights for the community.

 

Question for the authors

  1. mTurq2-Col4a1 mice are unviable when homozygous, suggesting that it is a hypomorphic allele. Do you expect defects in specific tissues that are more likely to require the mechanical properties of Collagen IV?
  2. As you pointed out in your manuscript, studies in Drosophila and C. elegans show rapid turnover with a half-life of about 7 hours (6,7). Your FRAP experiment includes data until 560 seconds after photobleaching, although you also show long-term live-imaging data in Figure 5 with a time span of 350 minutes. Would it be possible to analyze the recovery of Collagen IV over a longer period of time?

 

References

  1. Jayadev R, Sherwood DR. Basement membranes. Curr Biol. 2017 Mar 20;27(6):R207-R211. https://doi: 10.1016/j.cub.2017.02.006
  2. Yurchenco PD. Basement membranes: cell scaffoldings and signaling platforms. Cold Spring Harb Perspect Biol. 2011 Feb 1;3(2):a004911. https://doi: 10.1101/cshperspect.a004911
  3. Töpfer U. Basement membrane dynamics and mechanics in tissue morphogenesis. Biol Open. 2023 Aug 15;12(8):bio059980. https://doi: 10.1242/bio.059980
  4. Spurlin JW, Siedlik MJ, Nerger BA, Pang MF, Jayaraman S, Zhang R, Nelson CM. Mesenchymal proteases and tissue fluidity remodel the extracellular matrix during airway epithelial branching in the embryonic avian lung. Development. 2019 Aug 19;146(16):dev175257. https://doi: 10.1242/dev.175257
  5. Harunaga JS, Doyle AD, Yamada KM. Local and global dynamics of the basement membrane during branching morphogenesis require protease activity and actomyosin contractility. Dev Biol. 2014 Oct 15;394(2):197-205. https://doi: 10.1016/j.ydbio.2014.08.014
  6. Keeley DP, Hastie E, Jayadev R, Kelley LC, Chi Q, Payne SG, Jeger JL, Hoffman BD, Sherwood DR. Comprehensive Endogenous Tagging of Basement Membrane Components Reveals Dynamic Movement within the Matrix Scaffolding. Dev Cell. 2020 Jul 6;54(1):60-74.e7. https://doi: 10.1016/j.devcel.2020.05.022
  7. Matsubayashi Y, Sánchez-Sánchez BJ, Marcotti S, Serna-Morales E, Dragu A, Díaz-de-la-Loza MD, Vizcay-Barrena G, Fleck RA, Stramer BM. Rapid Homeostatic Turnover of Embryonic ECM during Tissue Morphogenesis. Dev Cell. 2020 Jul 6;54(1):33-42.e9. https://doi: 10.1016/j.devcel.2020.06.005

Tags: basement membrane, collagen, extracellular matrix, live-imaging, morphogenesis, mouse hair follicle, tissue deformation

Posted on: 30 October 2023 , updated on: 3 November 2023

doi: https://doi.org/10.1242/prelights.35875

Read preprint (No Ratings Yet)

Author's response

Danelle Devenport shared

Q1:mTurq2-Col4a1 mice are homozygously lethal, suggesting that it is a hypomorphic allele. Do you expect defects in specific tissues that are more likely to require the mechanical properties of Collagen IV?

This is a good point. Although we have carefully examined the structure of the dermal-epidermal junctional basement membrane in mTurq2-Col4a1 heterozygotes and do not detect any changes, we have not analyzed the structure of every BM in the animal. It is possible other BMs may be more sensitive to the presence of the tag and develop defects, but if present, such defects do not result in major developmental or health issues in heterozygous mice as they are both viable and fertile and appear similar to their wild type littermates.

Q2:As you pointed out in your manuscript, studies in Drosophila and C. elegans show rapid turnover with a half-life of about 7 hours (6,7). Your FRAP experiment includes data until 560 seconds after photobleaching, although you show long-term live-imaging data in Figure 5 with a time span of 350 minutes. Wouldn’t it make more sense to analyze the recovery of Collagen IV over a longer period of time?

We hypothesized that type IV collagen within an embryonic BM might turn over much faster than what has been reported in other systems due to the rapid growth of the embryo and expansion of the epidermal surface area during this time. Because of the way we performed the FRAP experiment – bleaching very small (>5µm) regions of interest and constantly monitoring over the imaging period – it was not possible to image for much longer than 10 minutes due to drift of the sample over time. If we were to bleach larger areas of the BM and follow recovery at much less frequent intervals, we could possibly monitor for hours rather than minutes and better determine the turnover rate.

 

Have your say

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

Sign up to customise the site to your preferences and to receive alerts

Register here

Also in the cell biology category:

Fetal brain response to maternal inflammation requires microglia

Bridget Elaine LaMonica Ostrem, Nuria Dominguez Iturza, Jeffrey Stogsdill, et al.



Selected by Manuel Lessi

Alteration of long and short-term hematopoietic stem cell ratio causes myeloid-biased hematopoiesis

Katsuyuki Nishi, Taro Sakamaki, Akiomi Nagasaka, et al.



Selected by Manuel Vicente, Anaisa Ferreira

Clusters of lineage-specific genes are anchored by ZNF274 in repressive perinucleolar compartments

Martina Begnis, Julien Duc, Sandra Offner, et al.



Selected by Silvia Carvalho

Also in the developmental biology category:

Alteration of long and short-term hematopoietic stem cell ratio causes myeloid-biased hematopoiesis

Katsuyuki Nishi, Taro Sakamaki, Akiomi Nagasaka, et al.



Selected by Manuel Vicente, Anaisa Ferreira

Temporal constraints on enhancer usage shape the regulation of limb gene transcription

Raquel Rouco, Antonella Rauseo, Guillaume Sapin, et al.



Selected by María Mariner-Faulí

OGT prevents DNA demethylation and suppresses the expression of transposable elements in heterochromatin by restraining TET activity genome-wide

Hugo Sepulveda, Xiang Li, Xiaojing Yue, et al.



Selected by Mansi

Also in the genetics category:

Temporal constraints on enhancer usage shape the regulation of limb gene transcription

Raquel Rouco, Antonella Rauseo, Guillaume Sapin, et al.



Selected by María Mariner-Faulí

A long non-coding RNA at the cortex locus controls adaptive colouration in butterflies

Luca Livraghi, Joseph J. Hanly, Elizabeth Evans, et al.

AND

The ivory lncRNA regulates seasonal color patterns in buckeye butterflies

Richard A. Fandino, Noah K. Brady, Martik Chatterjee, et al.

AND

A micro-RNA drives a 100-million-year adaptive evolution of melanic patterns in butterflies and moths

Shen Tian, Tirtha Das Banerjee, Jocelyn Liang Qi Wee, et al.



Selected by Isabella Cisneros

A revised single-cell transcriptomic atlas of Xenopus embryo reveals new differentiation dynamics

Kseniya Petrova, Maksym Tretiakov, Aleksandr Kotov, et al.



Selected by Rachel Mckeown

preLists in the cell biology category:

BSCB-Biochemical Society 2024 Cell Migration meeting

This preList features preprints that were discussed and presented during the BSCB-Biochemical Society 2024 Cell Migration meeting in Birmingham, UK in April 2024. Kindly put together by Sara Morais da Silva, Reviews Editor at Journal of Cell Science.

 



List by Reinier Prosee

‘In preprints’ from Development 2022-2023

A list of the preprints featured in Development's 'In preprints' articles between 2022-2023

 



List by Alex Eve, Katherine Brown

preLights peer support – preprints of interest

This is a preprint repository to organise the preprints and preLights covered through the 'preLights peer support' initiative.

 



List by preLights peer support

The Society for Developmental Biology 82nd Annual Meeting

This preList is made up of the preprints discussed during the Society for Developmental Biology 82nd Annual Meeting that took place in Chicago in July 2023.

 



List by Joyce Yu, Katherine Brown

CSHL 87th Symposium: Stem Cells

Preprints mentioned by speakers at the #CSHLsymp23

 



List by Alex Eve

Journal of Cell Science meeting ‘Imaging Cell Dynamics’

This preList highlights the preprints discussed at the JCS meeting 'Imaging Cell Dynamics'. The meeting was held from 14 - 17 May 2023 in Lisbon, Portugal and was organised by Erika Holzbaur, Jennifer Lippincott-Schwartz, Rob Parton and Michael Way.

 



List by Helen Zenner

9th International Symposium on the Biology of Vertebrate Sex Determination

This preList contains preprints discussed during the 9th International Symposium on the Biology of Vertebrate Sex Determination. This conference was held in Kona, Hawaii from April 17th to 21st 2023.

 



List by Martin Estermann

Alumni picks – preLights 5th Birthday

This preList contains preprints that were picked and highlighted by preLights Alumni - an initiative that was set up to mark preLights 5th birthday. More entries will follow throughout February and March 2023.

 



List by Sergio Menchero et al.

CellBio 2022 – An ASCB/EMBO Meeting

This preLists features preprints that were discussed and presented during the CellBio 2022 meeting in Washington, DC in December 2022.

 



List by Nadja Hümpfer et al.

Fibroblasts

The advances in fibroblast biology preList explores the recent discoveries and preprints of the fibroblast world. Get ready to immerse yourself with this list created for fibroblasts aficionados and lovers, and beyond. Here, my goal is to include preprints of fibroblast biology, heterogeneity, fate, extracellular matrix, behavior, topography, single-cell atlases, spatial transcriptomics, and their matrix!

 



List by Osvaldo Contreras

EMBL Synthetic Morphogenesis: From Gene Circuits to Tissue Architecture (2021)

A list of preprints mentioned at the #EESmorphoG virtual meeting in 2021.

 



List by Alex Eve

FENS 2020

A collection of preprints presented during the virtual meeting of the Federation of European Neuroscience Societies (FENS) in 2020

 



List by Ana Dorrego-Rivas

Planar Cell Polarity – PCP

This preList contains preprints about the latest findings on Planar Cell Polarity (PCP) in various model organisms at the molecular, cellular and tissue levels.

 



List by Ana Dorrego-Rivas

BioMalPar XVI: Biology and Pathology of the Malaria Parasite

[under construction] Preprints presented at the (fully virtual) EMBL BioMalPar XVI, 17-18 May 2020 #emblmalaria

 



List by Dey Lab, Samantha Seah

1

Cell Polarity

Recent research from the field of cell polarity is summarized in this list of preprints. It comprises of studies focusing on various forms of cell polarity ranging from epithelial polarity, planar cell polarity to front-to-rear polarity.

 



List by Yamini Ravichandran

TAGC 2020

Preprints recently presented at the virtual Allied Genetics Conference, April 22-26, 2020. #TAGC20

 



List by Maiko Kitaoka et al.

3D Gastruloids

A curated list of preprints related to Gastruloids (in vitro models of early development obtained by 3D aggregation of embryonic cells). Updated until July 2021.

 



List by Paul Gerald L. Sanchez and Stefano Vianello

ECFG15 – Fungal biology

Preprints presented at 15th European Conference on Fungal Genetics 17-20 February 2020 Rome

 



List by Hiral Shah

ASCB EMBO Annual Meeting 2019

A collection of preprints presented at the 2019 ASCB EMBO Meeting in Washington, DC (December 7-11)

 



List by Madhuja Samaddar et al.

EMBL Seeing is Believing – Imaging the Molecular Processes of Life

Preprints discussed at the 2019 edition of Seeing is Believing, at EMBL Heidelberg from the 9th-12th October 2019

 



List by Dey Lab

Autophagy

Preprints on autophagy and lysosomal degradation and its role in neurodegeneration and disease. Includes molecular mechanisms, upstream signalling and regulation as well as studies on pharmaceutical interventions to upregulate the process.

 



List by Sandra Malmgren Hill

Lung Disease and Regeneration

This preprint list compiles highlights from the field of lung biology.

 



List by Rob Hynds

Cellular metabolism

A curated list of preprints related to cellular metabolism at Biorxiv by Pablo Ranea Robles from the Prelights community. Special interest on lipid metabolism, peroxisomes and mitochondria.

 



List by Pablo Ranea Robles

BSCB/BSDB Annual Meeting 2019

Preprints presented at the BSCB/BSDB Annual Meeting 2019

 



List by Dey Lab

MitoList

This list of preprints is focused on work expanding our knowledge on mitochondria in any organism, tissue or cell type, from the normal biology to the pathology.

 



List by Sandra Franco Iborra

Biophysical Society Annual Meeting 2019

Few of the preprints that were discussed in the recent BPS annual meeting at Baltimore, USA

 



List by Joseph Jose Thottacherry

ASCB/EMBO Annual Meeting 2018

This list relates to preprints that were discussed at the recent ASCB conference.

 



List by Dey Lab, Amanda Haage

Also in the developmental biology category:

BSDB/GenSoc Spring Meeting 2024

A list of preprints highlighted at the British Society for Developmental Biology and Genetics Society joint Spring meeting 2024 at Warwick, UK.

 



List by Joyce Yu, Katherine Brown

GfE/ DSDB meeting 2024

This preList highlights the preprints discussed at the 2024 joint German and Dutch developmental biology societies meeting that took place in March 2024 in Osnabrück, Germany.

 



List by Joyce Yu

‘In preprints’ from Development 2022-2023

A list of the preprints featured in Development's 'In preprints' articles between 2022-2023

 



List by Alex Eve, Katherine Brown

preLights peer support – preprints of interest

This is a preprint repository to organise the preprints and preLights covered through the 'preLights peer support' initiative.

 



List by preLights peer support

The Society for Developmental Biology 82nd Annual Meeting

This preList is made up of the preprints discussed during the Society for Developmental Biology 82nd Annual Meeting that took place in Chicago in July 2023.

 



List by Joyce Yu, Katherine Brown

CSHL 87th Symposium: Stem Cells

Preprints mentioned by speakers at the #CSHLsymp23

 



List by Alex Eve

Journal of Cell Science meeting ‘Imaging Cell Dynamics’

This preList highlights the preprints discussed at the JCS meeting 'Imaging Cell Dynamics'. The meeting was held from 14 - 17 May 2023 in Lisbon, Portugal and was organised by Erika Holzbaur, Jennifer Lippincott-Schwartz, Rob Parton and Michael Way.

 



List by Helen Zenner

9th International Symposium on the Biology of Vertebrate Sex Determination

This preList contains preprints discussed during the 9th International Symposium on the Biology of Vertebrate Sex Determination. This conference was held in Kona, Hawaii from April 17th to 21st 2023.

 



List by Martin Estermann

Alumni picks – preLights 5th Birthday

This preList contains preprints that were picked and highlighted by preLights Alumni - an initiative that was set up to mark preLights 5th birthday. More entries will follow throughout February and March 2023.

 



List by Sergio Menchero et al.

CellBio 2022 – An ASCB/EMBO Meeting

This preLists features preprints that were discussed and presented during the CellBio 2022 meeting in Washington, DC in December 2022.

 



List by Nadja Hümpfer et al.

2nd Conference of the Visegrád Group Society for Developmental Biology

Preprints from the 2nd Conference of the Visegrád Group Society for Developmental Biology (2-5 September, 2021, Szeged, Hungary)

 



List by Nándor Lipták

Fibroblasts

The advances in fibroblast biology preList explores the recent discoveries and preprints of the fibroblast world. Get ready to immerse yourself with this list created for fibroblasts aficionados and lovers, and beyond. Here, my goal is to include preprints of fibroblast biology, heterogeneity, fate, extracellular matrix, behavior, topography, single-cell atlases, spatial transcriptomics, and their matrix!

 



List by Osvaldo Contreras

EMBL Synthetic Morphogenesis: From Gene Circuits to Tissue Architecture (2021)

A list of preprints mentioned at the #EESmorphoG virtual meeting in 2021.

 



List by Alex Eve

EMBL Conference: From functional genomics to systems biology

Preprints presented at the virtual EMBL conference "from functional genomics and systems biology", 16-19 November 2020

 



List by Jesus Victorino

Single Cell Biology 2020

A list of preprints mentioned at the Wellcome Genome Campus Single Cell Biology 2020 meeting.

 



List by Alex Eve

Society for Developmental Biology 79th Annual Meeting

Preprints at SDB 2020

 



List by Irepan Salvador-Martinez, Martin Estermann

FENS 2020

A collection of preprints presented during the virtual meeting of the Federation of European Neuroscience Societies (FENS) in 2020

 



List by Ana Dorrego-Rivas

Planar Cell Polarity – PCP

This preList contains preprints about the latest findings on Planar Cell Polarity (PCP) in various model organisms at the molecular, cellular and tissue levels.

 



List by Ana Dorrego-Rivas

Cell Polarity

Recent research from the field of cell polarity is summarized in this list of preprints. It comprises of studies focusing on various forms of cell polarity ranging from epithelial polarity, planar cell polarity to front-to-rear polarity.

 



List by Yamini Ravichandran

TAGC 2020

Preprints recently presented at the virtual Allied Genetics Conference, April 22-26, 2020. #TAGC20

 



List by Maiko Kitaoka et al.

3D Gastruloids

A curated list of preprints related to Gastruloids (in vitro models of early development obtained by 3D aggregation of embryonic cells). Updated until July 2021.

 



List by Paul Gerald L. Sanchez and Stefano Vianello

ASCB EMBO Annual Meeting 2019

A collection of preprints presented at the 2019 ASCB EMBO Meeting in Washington, DC (December 7-11)

 



List by Madhuja Samaddar et al.

EDBC Alicante 2019

Preprints presented at the European Developmental Biology Congress (EDBC) in Alicante, October 23-26 2019.

 



List by Sergio Menchero et al.

EMBL Seeing is Believing – Imaging the Molecular Processes of Life

Preprints discussed at the 2019 edition of Seeing is Believing, at EMBL Heidelberg from the 9th-12th October 2019

 



List by Dey Lab

SDB 78th Annual Meeting 2019

A curation of the preprints presented at the SDB meeting in Boston, July 26-30 2019. The preList will be updated throughout the duration of the meeting.

 



List by Alex Eve

Lung Disease and Regeneration

This preprint list compiles highlights from the field of lung biology.

 



List by Rob Hynds

Young Embryologist Network Conference 2019

Preprints presented at the Young Embryologist Network 2019 conference, 13 May, The Francis Crick Institute, London

 



List by Alex Eve

Pattern formation during development

The aim of this preList is to integrate results about the mechanisms that govern patterning during development, from genes implicated in the processes to theoritical models of pattern formation in nature.

 



List by Alexa Sadier

BSCB/BSDB Annual Meeting 2019

Preprints presented at the BSCB/BSDB Annual Meeting 2019

 



List by Dey Lab

Zebrafish immunology

A compilation of cutting-edge research that uses the zebrafish as a model system to elucidate novel immunological mechanisms in health and disease.

 



List by Shikha Nayar

Also in the genetics category:

BSDB/GenSoc Spring Meeting 2024

A list of preprints highlighted at the British Society for Developmental Biology and Genetics Society joint Spring meeting 2024 at Warwick, UK.

 



List by Joyce Yu, Katherine Brown

BSCB-Biochemical Society 2024 Cell Migration meeting

This preList features preprints that were discussed and presented during the BSCB-Biochemical Society 2024 Cell Migration meeting in Birmingham, UK in April 2024. Kindly put together by Sara Morais da Silva, Reviews Editor at Journal of Cell Science.

 



List by Reinier Prosee

9th International Symposium on the Biology of Vertebrate Sex Determination

This preList contains preprints discussed during the 9th International Symposium on the Biology of Vertebrate Sex Determination. This conference was held in Kona, Hawaii from April 17th to 21st 2023.

 



List by Martin Estermann

Alumni picks – preLights 5th Birthday

This preList contains preprints that were picked and highlighted by preLights Alumni - an initiative that was set up to mark preLights 5th birthday. More entries will follow throughout February and March 2023.

 



List by Sergio Menchero et al.

Semmelweis Symposium 2022: 40th anniversary of international medical education at Semmelweis University

This preList contains preprints discussed during the 'Semmelweis Symposium 2022' (7-9 November), organised around the 40th anniversary of international medical education at Semmelweis University covering a wide range of topics.

 



List by Nándor Lipták

20th “Genetics Workshops in Hungary”, Szeged (25th, September)

In this annual conference, Hungarian geneticists, biochemists and biotechnologists presented their works. Link: http://group.szbk.u-szeged.hu/minikonf/archive/prg2021.pdf

 



List by Nándor Lipták

2nd Conference of the Visegrád Group Society for Developmental Biology

Preprints from the 2nd Conference of the Visegrád Group Society for Developmental Biology (2-5 September, 2021, Szeged, Hungary)

 



List by Nándor Lipták

EMBL Conference: From functional genomics to systems biology

Preprints presented at the virtual EMBL conference "from functional genomics and systems biology", 16-19 November 2020

 



List by Jesus Victorino

TAGC 2020

Preprints recently presented at the virtual Allied Genetics Conference, April 22-26, 2020. #TAGC20

 



List by Maiko Kitaoka et al.

ECFG15 – Fungal biology

Preprints presented at 15th European Conference on Fungal Genetics 17-20 February 2020 Rome

 



List by Hiral Shah

Autophagy

Preprints on autophagy and lysosomal degradation and its role in neurodegeneration and disease. Includes molecular mechanisms, upstream signalling and regulation as well as studies on pharmaceutical interventions to upregulate the process.

 



List by Sandra Malmgren Hill
Close